Potent Anti-adhesion Barrier Combined Biodegradable Hydrogel with Multifunctional Turkish Galls Extract

Abstract

Clinically, postoperative adhesions are common and serious complications, which almost always happen after abdominal or pelvic surgery. The adhesion development process is accompanied by increased inflammatory cell infiltration and oxygen-free radical production. In this study, the naturally occurring antioxidative and anti-inflammatory compounds extracted from Turkish galls by ethyl acetate (GEA) were encapsulated into an injectable and biodegradable thermosensitive hydrogel. Antiadhesion efficacy of the barrier system (GEA-NP/H) was tested on a rat peritoneum injury-cecum abrasion model. Upon injection, the mildly viscous liquid formed a potent physical barrier over the injured cecum and peritoneum without any additional cross-linkers or light sources. Once formed, GEA-NP/H acted as a durable wound dressing for more than 5 days, as well as a sustained drug depot of GEA. The polymer hydrogel can be degraded and absorbed gradually. After 14 days, severe adhesion occurred among rats treated with normal saline and GEA-loaded nanoparticles (GEA-NP). Whereas, frequency of score 1 adhesion among the blank hydrogel group is 30%, and 90% of the rats from GEA-NP/H group exhibited no adhesion. In addition, pathological sections and scanning electron microscopy assay demonstrated that operative defects treated with GEA-NP/H suffered from mild oxidative stress and inflammatory damages at early days after injury, as well as accelerated wound healing and more mature mesothelial cell deposition at the 14th day in contrast to the blank hydrogel treatment. Therefore, the study provided an available biodegradable hydrogel barrier to effectively prevent postsurgical adhesion.

Comparison of Honey versus Polylactide Anti-Adhesion Barrier on Peritoneal Adhesion and Healing of Colon Anastomosis in Rabbits

“…The main effect of barriers is to separate injured serosal surfaces so that the mesothelial regeneration can be completed smoothly [19]. Beneficial anti-adhesion effects of barriers after abdominal surgeries [23], [26], [27] and gynecologic operations [28], [29] have been reported with acceptable results.…”

Recent Advances of Cell Membrane‐Coated Nanomaterials for Biomedical Applications

“…Furthermore, the anti‐adhesion strategy may have little influence on drug resistance of bacteria compared with antibiotics. [ 67 ] In one example, helicobacter pylori bacterial outer membrane was coated onto polymeric nanoparticles to obtain outer membrane‐coated nanoparticles (OM‐NPs) ( Figure A). [ 68 ] OM‐NPs inherited the surface antigens from the source bacteria and could inhibit adherence of bacteria to the host by competing with bacteria for occupying binding sites on the host.…”

Biomaterials to Prevent Post-Operative Adhesion

“…Studies have been conducted to prove the anti-adhesion effect of loading anti-inflammatory agents, e.g., NSAIDS such as ibuprofen [ 177 ], celecoxib [ 167 ], naproxen [ 178 ], and aspirin [ 179 ]. Research has also been conducted using natural anti-inflammatory agents, such as green tea extract [ 180 ] and Turkish galls extract [ 40 ].…”