Potensi Aktinomisetes Asal Tanah Perakaran Mangrove Segara Anakan Cilacap Sebagai Penghasil Antifungi Terhadap Yeast Patogen Candida albicans

Abstract

Isolation of actinomycetes has been done from mangrove soil sample of Segara Anakan Cilacap. This research aimed to know the potency of actinomycetes as an antifungal producer, and to measure antifungal activity produced by actinomycetes based on diameter zone inhibition and to know the characteristic of the bioactive compound with Thin Layer Chromatography method. The result of the study has been isolated 24 actinomycetes isolates, 15 of them had potential as an antifungal producer, which inhibited the growth of Candida albicans. Period of fermentation significantly affected to the activity of the antifungal compound. The highest inhibitory zone was formed by an antifungal extract from actinomycetes C with a range zone 19.7 mm in length at 14th days fermentation, which was extracted with ethyl acetate solvent (1: 1 v / v). The characteristic of a bioactive compound of the actinomycetes C ha s a Rf value 0.5 in the solvent n-butanol, acetic acid and water (3: 2: 1 v / v / v).

Optimization of Medium and Incubation Time in the Production of Antibacterial Compounds by Streptomyces sp. SA404

“…The main data was analyzed using ANOVA (Analysis of Variance) and then continued with the post hoc test using Tukey's HSD. [12] The isolates of Streptomyces sp. SA404 were sub cultured in SCNA medium, incubated in the room temperature for 7 days.…”

Characterization of Soil Actinomycetes From Malino Pine Forest Rhizosphere of South Sulawesi

“…Another distinctive characteristic of the 15 isolates from this study is the distinctive earthy odour that only belongs to the genus Streptomyces. Akbar et al, (2017) stated that this is because the main habitat of Streptomyces is on the ground. Streptomyces compose approximately 70 % of microorganisms in the soil and can be isolated in various environments, even from an unusual environment.…”

Effectiveness of Actinomycetes Isolates from Bogor Botanical Gardens Land as Antifungal against Candida albicans Growth in Vitro

“…Senyawa yang dihasilkan ini seperti Amfoterisin B, nistatin, dan natamisin dapat digunakan sebagai antijamur untuk mengobati kandidiasis (Oskay, 2009). (Akbar, Ryandini, & Kusharyati, 2017) menemukan 24 isolat Actinomycetes dari perakaran Mangrove Segara Anakan Cilacap dan 15 diantaranya berpotensi menghasilkan senyawa antifungi untuk menghambat pertumbuhan C. albicans. (Khoirina & Rahayu, 2014) menemukan 10 isolat Actinomycetes dari sampel pasir Gunung Merapi dan Actinomycetes tersebut dapat menghambat pertumbuhan C. albicans.…”

“…Besar kecilnya dari diameter zona hambat dapat dipengaruhi oleh berbagai hal antara lain jenis medium, jenis isolat, ketersediaan sumber karbon, pH medium, suhu inkubasi. (Akbar et al, 2017). Penelitian ini juga menggunakan media SDA untuk pertumbuhan jamur C. albicans, media ini digunakan karena dapat menumbuhkan C. albicans.…”

“…Penelitian sebelumnya yang telah dilakukan (Akbar et al, 2017) didapatkan 15 isolat yang ditemukan dari Segara Anakan Cilacap dapat berpotensi sebagai antifungi. Hasil yang diperoleh yaitu fermentasi isolat Actinomycetes berpotensi sebagai antifungi tertinggi pada hari ke-14 dengan menggunakan pelarut asetil asetat.…”