Potency of Stichopus hermanii extract as oral candidiasis treatment on epithelial rat tongue

Abstract

Background: Oral candidiasis is the most prominent oral fungal infection with Candida albicans (C. albicans) as 75% of ethiologic factor. Golden sea cucumbers (Stichopus hermanii) have been consumed by Asian community as folk medicine. It has been known to have antifungal and immunomodulator agent thus potential to be explored as treatment in oral candidiasis. Purpose: The aim of this study was to examine the potency of Stichopus hermanii extract as oral candidiasis treatment. Method: The study was an experimental laboratories research with post test only control group design. Thirty male wistar rats were divided into 5 groups i.e negative control, positive control and 3 treatment groups. Oral candidiasis condition were induced by spraying C. albicans suspension on dorsal tongue of wistar rats, once in 2 days for 14 days. The treatment groups were given Stichopus hermanii extract on the dose of 4.25 ml/kgBW, 8.5 ml/kgBW, 17 ml/kgBW once daily for 14 days. The expression of anti C. albicans antibody and TNF-α were examined by immunohistochemistry on ephitelial tongue. Data was analyzed by Manova and LSD test. Result: Anti C. albicans antibody expression were higher in positive control group than in negative control group while TNF-α expression were lower in positive control group than in negative control group (p<0.05). Treatment with Stichopus hermanii extract on all doses decreased the expression of anti C. albicans antibody and increased the expression of TNF-α (p<0.05). Conclusion: Stichopus hermanii extract decreased the expression of anti C. albicans antibody and increased the expression of TNF-α in epithelial rat tongue.

Golden sea cucumber extract revives glucose transporter-4 and interleukin-6 protein level in diabetic mouse muscle

“…Muscle secreted IL-6 in response to pro-inflammatory stimulation of macrophage tumor necrosis factor (TNF-α) [19]. Muscle IL-6 inhibited TNF-α signal transduction on inflammation, muscle wasting, protein degradation, and apoptosis [19,20]. We found a significant improvement of muscle IL-6 protein level in DM+extract mice group compared to DM.…”

“…The treatment of golden sea cucumber extract also improved muscle ability to control surrounding inflammation. Muscle secreted IL-6 in response to pro-inflammatory stimulation of macrophage tumor necrosis factor (TNF-α) [19]. Muscle IL-6 inhibited TNF-α signal transduction on inflammation, muscle wasting, protein degradation, and apoptosis [19,20].…”

STUDI EKSPERIMENTAL: PENURUNAN PROFIL NETROFIL DAN TNF-α SETELAH PEMBERIAN EKSTRAK TERIPANG EMAS (STICHOPUS HERMANNI)

“…Teripang, terutama Stichopus hermanni diketahui memiliki berbagai manfaat sebagai antijamur, antibakteri, antiinflamasi dan antioksidan (11,12). Manfaat sebagai anti jamur telah banyak diteliti terutama dalam pengobatan oral candidiasis (13). Kemampuan dari ekstrak teripang emas dalam hambatan anti jamur diperkirakan dengan adanya kandungan saponin (glikosida triterpen) (14).…”

“…Langkah akhir adalah pemisahan filtrat dari pelarutnya untuk mendapatkan ekstrak, proses ini dilakukan dengan alat rotary evaporator (13).…”

<p>Daya antibakteri asam palmitat bawang putih (Allium sativum) terhadap Streptococcus mutans ATCC 25175</p><p>Antibacterial activity of garlic (Allium sativum) palmitic acid towards Streptococcus mutans ATCC 25175</p>

“…12 Asam lemak mudah dilarutkan dengan pelarut non-polar. 13 Kloroform yang digunakan sebagai pelarut asam palmitat dalam penelitian ini merupakan pelarut non-polar yang memiliki jenis kepolaran yang sama dengan asam palmitat (zat terlarutnya), oleh karena itu asam palmitat terlarut dalam kloroform. Aktivitas antibakteri asam lemak tidak jenuh rantai panjang telah diketahui selama bertahun-tahun, seperti asam linoleat dan oleat yang bersifat bakterisidal terhadap mikroorganisme patogen, termasuk Methicillin-Resistant, Staphylococcus aureus, Helicobacter pylori, dan Candida albicans.…”