Potency of Black Soybean (Glycine max (L.) Merr) Extract and Daidzein as Antioxidant and Antihyaluronidase

Abstract

Black soybean (Glycine max (L.) Merr.) is a plant that is widely planted and consumed in Indonesia. In addition, black soybean has unique content of isoflavones, such as daidzein, which is one of the active compounds that have the effect of fighting free radicals and can inhibit the aging process. The purpose of this study is to analyze the antioxidant potency possessed by black soybean extract (BSE) and daidzeinin inhibiting aging of the skin. The method used is a colorimetric test. The type of antioxidant test used is H2O2 scavenging and inhibiting the activity of the hyaluronidase enzyme for antiaging. BSE has better effectiveness of H2O2 scavenging (IC50: 286.24±11.16 (µg/mL)) than daidzein compound (IC50: 366.16±2.54 (µg/mL)). In the inhibition of hyaluronidase enzyme, the daidzein has more effective activities (IC50: 95.80±3.98 (µg/mL)) compared to BSE (IC50: 152.56±13.98 (µg/mL)). The antioxidant and anti-aging activities possessed by BSE make it possible to be used as a cosmetic ingredient for skin aging therapy.

Potentials of rose (Rosa damascena) petals and receptacles extract as antioxidant and antihyaluronidase

“…RPE and RRE yields were 88.56 g and 2.72 g, respectively. The extracts were stored at -20°C (Asan et al, 2019;Girsang et al, 2019;Liana et al, 2019;Vrianty et al, 2019;Widowati et al, 2018b).…”

“…Then it was incubated again in the same condition for 10 mins. The absorbances were determined at 510 nm (Asan et al, 2019;Jusri et al, 2019;Liana et al, 2019;Utami et al, 2017). The scavenging activities percentage of samples were calculated using this following formula : H 2 O 2 Scavenging Activity (%) = (Ac -As)/Ac × 100 Ac: control absorbance As: sample absorbance…”

Antioxidant and Elastase Inhibitor Potential of Petals and Receptacle of Rose Flower (Rosa damascena)

“…The both extract RPE and RRE were later diluted in dimethyl sulfoxide (DMSO) with following concentrations: 5000, 2500, 1250, 625, 312.50, 156.25, 1000, 500, 250, 125, 62.50, and 31.25 µg/mL. This dilution are later called working concentrations (Mahadi et al, 2019;Vrianty et al, 2019;Siregar et al, 2019;Girsang et al, 2019;Liana et al, 2019;Asan et al, 2019;Prahastuti et al, 2020).…”

“…Some examples of plants that have antioxidant and antiaging effects are roselle roses (Hisbiscus sabdarifa) (Widowati et al, 2017), jasmine (Jasminum sambac) (Widowati et al, 2018b), ripe sesoot fruit (Garcinia picrorrhiza Miq.) (Utami et al, 2018), black soybeans (Glycine max [L.] Merr) (Asan et al, 2019), dragon fruit peel (Hylocereus polyrhizus) (Liana et al, 2019), pineapple (Vrianty et al, 2019), and zalacca skin (Salacca zalacca (Gaert.) Voss) (Girsang et al, 2019).…”

“…Voss) (Girsang et al, 2019). Sources of antioxidants are mangosteen peel (Garcinia mangostana L.) (Widowati et al, 2014), black tea, green tea, oolong tea (Camelia sinensis L.) (Widowati et al, 2015), green meniran (Phyllanthus niruri) (Rusmana et al, 2017), black soybean (Glycine max L. Merr) (Asan et al, 2019), dragon fruit peel (Hylocereus polyrhizus) (Liana et al, 2019), pineapple (Vrianty et al, 2019), and zalacca bark (Salacca zalacca (Gaert.) Voss) (Girsang et al, 2019).…”