Postnatal changes in glucose transporter 3 expression in the dentate gyrus of the C57BL/6 mouse model

Abstract

In this study, we observed the ontogenetic changes in glucose transporter 3 (GLUT3) immunoreactivity, a major neuronal GLUT, in the dentate gyrus of mouse brains at various ages: postnatal day (P) 1, 7, 14, 28, and 56. At P1, cresyl violet staining showed abundant neurons in the dentate gyrus, whereas the granule cell layer was ill-defined. At P7, the granule cell layer was observed, and cresyl violet-positive cells were dispersed throughout the polymorphic layer. At P14, the granule cell layer was well-defined, and cresyl violet positive cells were detected abundantly in the polymorphic layer. At P28 and P56, cresyl violet-positive cells were observed in the granule cell layer, as well as in the polymorphic layer. At P1, GLUT3 immunoreactivity was detected in the dentate gyrus. At P7, GLUT3 immunoreactive cells were scattered in the polymorphic and molecular layer. However, at P14, GLUT3 immunoreactivity was observed in the polymorphic layer as well as subgranular zone of the dentate gyrus. At P28, GLUT3 immunoreactivity was detected in the polymorphic layer of the dentate gyrus. At P56, GLUT3 immunoreactivity was observed predominantly in the subgranular zone of the dentate gyrus. GLUT3 immunoreactive cells were mainly colocalized with doublecortin, which is a marker for differentiated neuroblasts, in the polymorphic layer and subgranular zone of dentate gyrus at P14 and P56. These results suggest that the expression of GLUT3 is closely associated with postnatal development of the dentate gyrus and adult neurogenesis.

Lack of evidence in neurite growth in the gerbil hippocampal CA1 region 15 days after transient forebrain ischemia

“…In contrast, GLUT3 immunoreactive structures were co-localized with DCXimmunoreactive neuroblasts in the dentate gyrus. This result is consistent with our previous study that GLUT3 immunoreactivity is detected in the DCX-immunoreactive neuroblasts in postnatal mouse brain (Jung et al 2016) and seven days post-ischemic gerbil brain (Yoo, Lee, et al 2016). This result suggests that the endogenous repair program was activated by the transient forebrain ischemia in order to promote the repair of the neuronal damage.…”

“…In the gerbil dentate gyrus, GLUT3 immunoreactivity is significantly increased seven days after ischemia (Yoo, Lee et al, 2016). In addition, GLUT3 immunoreactivity colocalizes with doublecortin (DCX)-immunoreactive neuroblasts in the dentate gyrus of postnatal mice dentate gyrus (Jung et al 2016) and ischemia gerbil dentate gyrus (Yoo, Lee et al 2016). Neuronal GLUT3 levels in the hippocampus of middle-aged rats are lower than the levels observed in the hippocampus of young-adult rats (Wang et al 2012).…”

Melatonin ameliorates cuprizone‐induced reduction of hippocampal neurogenesis, brain‐derived neurotrophic factor, and phosphorylation of cyclic AMP response element‐binding protein in the mouse dentate gyrus

“…In the present study, we found that GLUT3 immunoreactivity was significantly decreased in the subgranular zone of the dentate gyrus after a 6‐week cuprizone diet compared to that after a normal diet. This means that cuprizone administration affects glucose transport and utilization in the proliferating cells or neuroblasts because GLUT3 immunoreactivity increases when neurogenesis is high, similar to the situation in newborn neurons (Jung et al, ) and in brain ischemia‐induced compensatory neurogenesis (Yoo et al, ). In the cuprizone + melatonin group, immunoreactivity of GLUT3 in the hippocampal dentate gyrus significantly increased compared to that in the cuprizone group.…”

Decrease in glucose transporter 1 levels and translocation of glucose transporter 3 in the dentate gyrus of C57BL/6 mice and gerbils with aging

“…In adult dentate gyrus, GLUT3 expression was mainly found in the subgranular zone of the dentate gyrus. In the previous study, our colleagues showed that GLUT3 is expressed in DCX-immunoreactive neuroblasts during postnatal development in the mouse dentate gyrus [ 18 ] and in normal and ischemic gerbil dentate gyrus [ 31 ]. GLUT3 expression can be activated by the binding between the promoter region and the phosphorylated cyclic AMP-regulatory element-binding (pCREB) protein and the mouse Y box-binding protein-1 [ 32 ].…”

“…Morphological studies have showed that GLUT3 immunoreactivity is found in the mossy fibers of the CA3 region, stratum radiatum of the CA1 region, and the granule cell layer and hilar region of the dentate gyrus of the mouse hippocampus [ 16 ] and rat [ 17 ]. In our previous study, we showed that GLUT3 immunoreactivity is found in the subgranular zone of the dentate gyrus and GLUT3 is colocalized with doublecortin (DCX)-immunoreactive neuroblasts in the dentate gyrus of the postnatal mouse brain [ 18 ]. In this regard, GLUT3 can also modulate hippocampal neurogenesis in the subgranular zone of the dentate gyrus.…”