Decrease in glucose transporter 1 levels and translocation of glucose transporter 3 in the dentate gyrus of C57BL/6 mice and gerbils with aging

Lee, Kwon Young; Yoo, Dae Young; Jung, Hyo Young; Baek, Loktam; Lee, Hangyul; Kwon, Hyun Jung; Chung, Jin Young; Kang, Seok Hoon; Kim, Dae Won; Hwang, In Koo; Choi, Jung Hoon

doi:10.5625/lar.2018.34.2.58

Cited by 10 publications

(6 citation statements)

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“…Glucose transporter 1 (GLUT1) belong to a family of proteins managing glucose homeostasis. Astrocytes and endothelial cells are necessary for the entry of glucose into the brain via the blood-brain barrier (Lee et al, 2018). The observed reduction of GLUT1 in old brain is consistent with the findings of Byers, Howard, and Wang (2017) in ageing chicken.…”

Section: Sabrysupporting

confidence: 87%

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

Sabry

2020

Acta Zoologica

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The present research explores the ageing impact of Columba livia domestica on retina and cerebellum. It is an available and a widespread bird species. Three ageing groups (n = 5) of male Columba were collected at about three weeks, six months and five years old. Histological studies were made. Morphometric measurement of both retinal and cerebellar cortex layer thicknesses has been determined. For evaluation of the neuronal synapses, quantitative GFAP biochemically and immunohistochemically staining was performed. Also, biochemical analysis of glucose transporter 1, serotonin and dopamine was carried out to demonstrate their functional activity.Flow cytometric assessments of quantitative cell death were accomplished by using annexin V. The present findings revealed quantitative and qualitative increase of GFAP parallel with decrease of serotonin, dopamine and glucose transporter 1 as well as increase of UR and LR annexin V reflecting cell death in old groups. These were confirmed by damaging of the retina and cerebellar layers. The present findings concluded that retinal and cerebellar cells associated with ageing were determined by sharp rise in both cell death and GFAP levels. Additionally, a decrease of dopamine, serotonin and GluT1 level manifested maybe led to functional alteration of both retina and brain. K E Y W O R D Sageing, annexin V, cerebellum, Columba livia domestica, histology, retina | 395 SABRY triggered during flight by acquiring information about the optic flow (Wylie et al., 2018). In pigeons, the visual motion takes place throughout the retina via self-motion as self-rotation and self-translation is affected by information inputs in vestibulocerebellum (Wylie, 2013). Both retina and cerebellum also play an essential role for locomotion of pigeons for food collection and avoiding predators (Dhawan, 1991). Consequently, both retina and cerebellum are of great importance in managing the pigeon activity.Birds showed higher metabolic rate (2-2.5 times) (Butler, 2016) and two-to-three times longer life spans compared to mammals of the same size (Travin & Feniouk, 2016). These may result from certain adaptation for inhibiting ageing-related damage induced by reactive oxygen species and formation of glycation end products (Holmes, Fluckiger, & Austad, 2001). Also, birds characterized by their higher body temperature (3-5°C) and blood glucose (2-4 times) than mammals (Ritchie, Harrison, & Harrison, 1994) of similar body mass. Mammalian and avian species of more than one kilogram showed blood glucose concentration within the range of about 135 and 275 mg/dl, respectively (Braun & Sweazea, 2008). These manifestations supposed to accelerate ageing in birds due to the production of glycosylated proteins via Maillard reaction (Monnier, 1990;Kristal & Yu, 1992). However, they show increased longevity (Skujina, McMahon, Lenis, Gkoutos, & Hegarty, 2016). This arouses curiosity about the comparable changes of ageing among avian and mammalian species (Holmes et al., 2001) and attracts many authors such as Holme...

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Section: Sabrysupporting

confidence: 87%

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

Sabry

2020

Acta Zoologica

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“…In other organisms GLUT3 expression was shown to be detectable in multiple neuronal cell types, especially in the hippocampus of mice; this included, but was not restricted to GABAergic cells (Cembrowski et al, 2016a,b, 2018; Shah et al, 2016). Moreover, there is a debate whether GLUT3 might be expressed at low levels by astrocytes in mammalian systems, and that these cells can up-regulate their expression during certain conditions (Iwabuchi et al, 2014; Wang et al, 2016; Lee et al, 2018). The zebrafish CNS contains similar types of glia cells as the mammalian CNS, with the exception of astrocytes and radial glia (Lyons and Talbot, 2015).…”

Section: Discussionmentioning

confidence: 99%

Transcript Analysis of Zebrafish GLUT3 Genes, slc2a3a and slc2a3b, Define Overlapping as Well as Distinct Expression Domains in the Zebrafish (Danio rerio) Central Nervous System

Lechermeier

Zimmer

Lüffe

et al. 2019

Front. Mol. Neurosci.

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The transport of glucose across the cell plasma membrane is vital to most mammalian cells. The glucose transporter (GLUT; also called SLC2A) family of transmembrane solute carriers is responsible for this function in vivo . GLUT proteins encompass 14 different isoforms in humans with different cell type-specific expression patterns and activities. Central to glucose utilization and delivery in the brain is the neuronally expressed GLUT3. Recent research has shown an involvement of GLUT3 genetic variation or altered expression in several different brain disorders, including Huntington’s and Alzheimer’s diseases. Furthermore, GLUT3 was identified as a potential risk gene for multiple psychiatric disorders. To study the role of GLUT3 in brain function and disease a more detailed knowledge of its expression in model organisms is needed. Zebrafish ( Danio rerio ) has in recent years gained popularity as a model organism for brain research and is now well-established for modeling psychiatric disorders. Here, we have analyzed the sequence of GLUT3 orthologs and identified two paralogous genes in the zebrafish, slc2a3a and slc2a3b . Interestingly, the Glut3b protein sequence contains a unique stretch of amino acids, which may be important for functional regulation. The slc2a3a transcript is detectable in the central nervous system including distinct cellular populations in telencephalon, diencephalon, mesencephalon and rhombencephalon at embryonic and larval stages. Conversely, the slc2a3b transcript shows a rather diffuse expression pattern at different embryonic stages and brain regions. Expression of slc2a3a is maintained in the adult brain and is found in the telencephalon, diencephalon, mesencephalon, cerebellum and medulla oblongata. The slc2a3b transcripts are present in overlapping as well as distinct regions compared to slc2a3a . Double in situ hybridizations were used to demonstrate that slc2a3a is expressed by some GABAergic neurons at embryonic stages. This detailed description of zebrafish slc2a3a and slc2a3b expression at developmental and adult stages paves the way for further investigations of normal GLUT3 function and its role in brain disorders.

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“…This may limit nutrient, including glucose, import into the brain. (ii) Age-related reduction in the expression of glucose transporters as well as expression and/or activity of enzymes involved in glycolysis were reported (Figure 1) [15][16][17].…”

Section: Alteration In Glucose Metabolismmentioning

confidence: 95%

Impairments in Brain Bioenergetics in Aging and Tau Pathology: A Chicken and Egg Situation?

Grimm

2021

Cells

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The brain is the most energy-consuming organ of the body and impairments in brain energy metabolism will affect neuronal functionality and viability. Brain aging is marked by defects in energetic metabolism. Abnormal tau protein is a hallmark of tauopathies, including Alzheimer’s disease (AD). Pathological tau was shown to induce bioenergetic impairments by affecting mitochondrial function. Although it is now clear that mutations in the tau-coding gene lead to tau pathology, the causes of abnormal tau phosphorylation and aggregation in non-familial tauopathies, such as sporadic AD, remain elusive. Strikingly, both tau pathology and brain hypometabolism correlate with cognitive impairments in AD. The aim of this review is to discuss the link between age-related decrease in brain metabolism and tau pathology. In particular, the following points will be discussed: (i) the common bioenergetic features observed during brain aging and tauopathies; (ii) how age-related bioenergetic defects affect tau pathology; (iii) the influence of lifestyle factors known to modulate brain bioenergetics on tau pathology. The findings compiled here suggest that age-related bioenergetic defects may trigger abnormal tau phosphorylation/aggregation and cognitive impairments after passing a pathological threshold. Understanding the effects of aging on brain metabolism may therefore help to identify disease-modifying strategies against tau-induced neurodegeneration.

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Decrease in glucose transporter 1 levels and translocation of glucose transporter 3 in the dentate gyrus of C57BL/6 mice and gerbils with aging

Cited by 10 publications

References 35 publications

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

Comparative study about ageing effect on retina and cerebellum of Columba livia domestica

Transcript Analysis of Zebrafish GLUT3 Genes, slc2a3a and slc2a3b, Define Overlapping as Well as Distinct Expression Domains in the Zebrafish (Danio rerio) Central Nervous System

Impairments in Brain Bioenergetics in Aging and Tau Pathology: A Chicken and Egg Situation?

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