Postechiella marina gen. nov., sp. nov., isolated from seawater

Abstract

A Gram-staining-negative, strictly aerobic, non-motile, yellow-pigmented bacterium, designated strain M091T , was isolated from seawater at Damupo beach in Pohang, Republic of Korea, and investigated using a polyphasic taxonomic approach. The novel strain grew optimally at 25 6C, pH 7.0-8.0, and in the presence of 3 % (w/v) NaCl. In a phylogenetic analysis based on 16S rRNA gene sequences, strain M091 T formed a lineage within the family Flavobacteriaceae that was distinct from the most closely related genera of Flaviramulus (95.1 % sequence similarity), Algibacter (94.9-93.9 %), Mariniflexile (94.8-94.2 %), Winogradskyella (94.8-93.2 %), and Tamlana (94.7-92.9 %). The polar lipid profile of the novel strain comprised phosphatidylethanolamine, two unidentified aminolipids, one unidentified phospholipid and seven unidentified lipids. The predominant cellular fatty acids were iso-C 15 : 0 (20.5 %), iso-C 17 : 0 3-OH (15.4 %), iso-C 15 : 0 3-OH (12.4 %), C 15 : 0 (10.9 %) and iso-C 15 : 1 G (9.9 %). The genomic DNA G+C content of strain M091 T was 34.4 mol% and the major respiratory quinone was MK-6. Based on phenotypic and genotypic data, strain M091 T represents a new genus and novel species in the family Flavobacteriaceae, for which the name Postechiella marina gen. nov., sp. nov. is proposed. The type strain of the type species is M091 T (5KCTCThe family Flavobacteriaceae was first proposed by Jooste (1985) and subsequently described and emended by Bernardet et al. (1996Bernardet et al. ( , 2002. This family is one of the major phylogenetic lineages within the phylum Bacteroidetes (Garrity & Holt, 2001). At the time of writing, the family comprised more than 90 recognized genera (http://www. bacterio.cict.fr/). Many members of this family were isolated from various marine environments and Antarctic habitats (Bowman et al., 1997(Bowman et al., , 1998Bowman, 2000;Bowman & Nichols, 2002Cho & Giovannoni, 2004;Nedashkovskaya et al., 2004;Nichols et al., 2005;Pinhassi et al., 2006). These bacteria are known to play an important role in the uptake and degradation of complex dissolved and particulate organic matter in marine ecosystems (Kirchman, 2002). In the course of investigations of bacterial diversity in seawater, a novel yellow-pigmented bacterium, designated strain M091 T , was isolated from a seawater sample collected at Damupo beach in Pohang, Korea. On the basis of phylogenetic analysis, strain M091T formed a distinct cluster closely related to members of the genera Flaviramulus, Algibacter, Mariniflexile, Winogradskyella, Lacinutrix and Tamlana of the phylum Bacteroidetes. The taxonomic characterization of strain M091 T , using a polyphasic approach, is described in this report. Strain M091T was isolated from seawater collected at Damupo beach in Pohang, Republic of Korea (36 u 19 55.010 N 129u 349 50.980 E), by using a standard serial dilution plating technique on marine agar 2216 (MA; Difco). The strain was initially grown at 25 u C for 5 days and then routinely maintained on MA, with subculture every 3 ...

Identification and characterization of 3,6-anhydro-L-galactose dehydrogenases belonging to the aldehyde dehydrogenase superfamily from marine and soil microorganisms

“…Strains and culture conditions P. marina M091 (KCTC 23537) was cultivated using previously described methods [16]. Pseudoalteromonas atlantica T6c (ATCC BAA-1087) was cultivated at 26°C in marine broth 2216 (Difco, Detroit, MI, USA).…”

“…For these purposes, microorganisms that hydrolyze and metabolize agar as a carbon and energy source were screened from seawater. Recently, we reported the characterization of one novel microorganism belonging to a new genus, Postechiella marina M091, which was isolated from a seawater sample obtained at Damupo beach in Pohang, Korea [16]. The phylogenetic analysis of the 16S rRNA of P. marina M091 showed that it belongs to the class Flavobacteria in the phylum Bacteroidetes.…”

Assessing of Flexible Packaging Integrity: Using the Aerosolization Bacteria

“…It has been suggested that these bacteria could be used as one of the standards to test biosampling devices . E. Coli DH 5α is a motile, straight rod, that ranges in size from 1.1–1.5 × 2.0–6.0 µm . Motile organisms display a variety of shapes via expansion and contraction, as well as modes of locomotion, thereby allowing the organism to enter areas not accessible to nonmotile organisms.…”

“…28 E. Coli DH 5α is a motile, straight rod, that ranges in size from 1.1-1.5 × 2.0-6.0 μm. 30 Motile organisms display a variety of shapes via expansion and contraction, as well as modes of locomotion, thereby allowing the organism to enter areas not accessible to nonmotile organisms. In contrast, such characteristics may not be exhibited by nonmotile organisms.…”

Unusual metabolism of 3,6-anhydro-L-galactose in Vibrio sp. EJY3 and in E. coli containing two Vibrio sp. EJY3 genes

“…P. marina M091, which was isolated from a seawater sample obtained at Damupo beach in Pohang, Korea, is a novel agar-degrading microorganism belonging to a new genus within the family Flavobacteriaceae [9].…”