2016
DOI: 10.1002/pts.2191
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Assessing of Flexible Packaging Integrity: Using the Aerosolization Bacteria

Abstract: Package integrity is a critical factor to ensure that product sterility is maintained over its entire shelf life. An exposure chamber method was developed to determine the integrity of flexible packaging systems, i.e. flexible multilayer linear low‐density polyethylene/nylon pouches with a 100, 50, 25 or 15 µm micro‐channel with 5 mm length defect in the sealing area. Viable cells of Staphylococcus aureus and Escherichia coli have been utilized as model organisms. The aerosol was generated by a nebulizer, abou… Show more

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Cited by 11 publications
(7 citation statements)
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“…The cutoff for the microbial ingress study was 15 lm, which was identical to the cutoff for the dye penetration study for this particular type of leak. The samples with defect sizes of 50 and 25 lm took similar amounts of time to show microbial contamination inside a package, which means that a clear micro-channel for microbial ingress was 25 lm [15]. However, the pouches with 15 lm micro-channels did not show microbial growth.…”
Section: Comparison Of Bioaerosol Challenge Test and Dye Penetration Testmentioning
confidence: 94%
See 1 more Smart Citation
“…The cutoff for the microbial ingress study was 15 lm, which was identical to the cutoff for the dye penetration study for this particular type of leak. The samples with defect sizes of 50 and 25 lm took similar amounts of time to show microbial contamination inside a package, which means that a clear micro-channel for microbial ingress was 25 lm [15]. However, the pouches with 15 lm micro-channels did not show microbial growth.…”
Section: Comparison Of Bioaerosol Challenge Test and Dye Penetration Testmentioning
confidence: 94%
“…A pouch with no defect was injected with 0.2 mL of a 10 3 CFU/mL solution of the organisms to serve as a positive control. We applied this method to another type of packaging in our previous work [15].…”
Section: Microbial Ingress Proceduresmentioning
confidence: 99%
“…The sealing reliability of metal foils and plastic films is important when they are used to package foods, medicines and dangerous goods, with unexpected pinholes and cracks in these materials leading to leakages 1–17 . Two mechanisms that gases pass through these leak holes are considered 1,2 .…”
Section: Introductionmentioning
confidence: 99%
“…A great number of researches have been done on rigid sandwich structure by using honeycomb, metals and 3D woven fabrics [14]. Different from rigid sandwich structure, flexible sandwich structure has drawn much attention in individual protection field owing to the excellent user comfort and effective impact resistance over the protected body parts [5]. The flexibility of fibers and yarns endows textile material with unique advantages over impact protection; therefore, special attention has been devoted to make protective material with textile structures [6].…”
Section: Introductionmentioning
confidence: 99%