Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Polisseni, Juliana; Sá, Wanderlei Ferreira de; Guerra, Martha de Oliveira; Machado, M. A.; Serapião, R. V.; Carvalho, Bruno Campos de; Camargo, L. S. A.; Peters, Vera Maria

doi:10.1016/j.fertnstert.2008.10.023

Cited by 17 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A correlation has been found between blastocyst quality score and the proportion of embryos developing to the blastocyst stage, rate of development, metabolism, cell number in the blastocyst, and incidence of cell division and cell death (Gelber et al 2011; Kim et al 2011; Matsuura et al 2010; Polisseni et al 2010). In our study we observed a higher number of cells per embryo and larger blastocyst diameter after coculture with iMSC or iMEF when compared to controls.…”

Section: Discussionmentioning

confidence: 96%

Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos

Jasmin

Peters

Spray

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

Self Cite

View full text Add to dashboard Cite

Summary Despite advances in assisted reproduction techniques, the poor quality and failures in embryo in vitro development remain as drawbacks resulting in low pregnancy rate. Mouse embryonic fibroblasts (MEFs) have been widely used to support embryonic stem cells. Mesenchymal cells (MSCs) have also been shown to release bioactive factors. In the present study we have evaluated the ability of MSCs and MEFs to support early development of mouse embryos. The embryos were cultivated alone or in coculture with inactivated MSC or MEF for 4 days. After 4 days in culture the percentage of blastocyst formation in coculture with MSC (91.7±4.3%) or MEF (95.1±3.3%) was higher than in the control group (72.2±9.0%). We did not observe any difference in proliferation or apoptosis. However, the blastocysts cocultured with MSC or MEF presented a significantly higher number of cells within the inner cell mass per embryo when compared to controls. The MSC and MEF groups presented also a higher cell number and diameter when compared to the CTRL. In summary, our data indicate that coculture with MSC or MEF improve early embryonic development and quality in vitro.

show abstract

Section: Discussionmentioning

confidence: 96%

Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos

Jasmin

Peters

Spray

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

Self Cite

View full text Add to dashboard Cite

show abstract

“…The consistency of these results suggests that there is no inhibitory effect from components in the embryo lysate and hence a DNA purification step before the WGA is not necessary. The results presented here suggest that the pre-treatment of samples with proteinase K claimed to destroy amplification inhibitors before WGA and improve efficiency [13] also seems to be unnecessary.…”

Section: Discussionmentioning

confidence: 89%

Assessment of MDA efficiency for genotyping using cloned embryo biopsies

et al. 2013

View full text Add to dashboard Cite

The possibility to genotype embryos prior to implantation would have advantages for increasing the speed of selection of cattle. Reliable genotyping requires more DNA than can be obtained from biopsies of embryos, if they are to remain viable. Multiple displacement amplification (MDA) is a whole genome amplification technique used to increase the amount of DNA from biopsies for analysis. Reduced genome coverage resulting in Allele Drop Out (ADO) at heterozygous loci or missing genotypes are drawbacks of MDA. The present article describes the correlation between the input DNA quantity or embryo biopsy size and MDA success. Missing genotypes and ADO drastically increased when fewer than 30-40 cells or the genomic equivalents were used. However, embryo viability was found to be reduced if biopsied with more than 10 cells. Therefore, in vitro cell culture was investigated as a means to increase the number of cells available and the genotyping reliability.

show abstract

“…Polisseni et al reported on the use of DNA fingerprinting for eight chromosomes from 11 paired BF-DNA samples separated from whole embryo DNA [11]. This was used to determine the concordance of polymorphic loci between paired samples, showing that 100% of the loci successfully amplified using samples from the whole embryo, but DNA from the BF was of inferior quality and unable to provide a complete DNA fingerprinting analysis.…”

Section: Discussionmentioning

confidence: 99%

Detection and analysis of DNA material in human blastocoel fluid

Shangguan¹,

He²,

Li³

et al. 2017

Biomed Genet Genomics

View full text Add to dashboard Cite

Preimplantation genetic diagnosis (PGD) is becoming a widely-accepted technique during in vitro fertilization (IVF). However, a disadvantage of PGD is the invasive biopsy methods used to sample embryonic cells or polar bodies. Recent studies have found that genetic material can be detected in blastocoel fluid (BF) and culture medium. In our study, BF and trophectoderm (TE) cells were simultaneously collected from the same donated human blastula. To generate enough DNA for analysis, we used multiple displacement amplification (MDA) based whole genome amplification (WGA). MDA-WGA samples were probed with primers designed to identify spinal muscular atrophy (SMA) and phenylpropionate ketoneuria (PKU), plus the Y chromosome sex-determining region (SRY). This demonstrated that DNA fragments were present in each of the TE and BF samples (7/7). The positive PCR amplification rates for SMA, PKU, SRY and β-actin in BF were 42.9% (3/7), 60% (3/5), 42.9% (3/7) and 71.4% (5/7) respectively, but the positive rate of amplification with TE samples was 100% (7/7), 100% (7/7), 71.43% (5/7) and 100% (7/7). After sequencing, identical alleles were found in matched BF and TE samples. In summary, BF-DNA could be detected using MDA-WGA and PCR, and sequences in PCR positive samples were identical in matched BF-DNA and TE samples.

show abstract

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Cited by 17 publications

References 20 publications

Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos

Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos

Assessment of MDA efficiency for genotyping using cloned embryo biopsies

Detection and analysis of DNA material in human blastocoel fluid

Contact Info

Product

Resources

About