Possible Animal Origin of Human-Associated, Multidrug-Resistant, Uropathogenic Escherichia coli

Ramchandani, Meena; Manges, Amee R.; DebRoy, Chitrita; Smith, Sherry P.; Johnson, James R.; Riley, Lee W.

doi:10.1086/426819

Cited by 140 publications

(126 citation statements)

References 26 publications

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“…Although this isolate's virulence profile resembled that of the outbreak isolates, including the EAEC genes, the isolate differed from the outbreak isolates according to astA status (positive), biotype (D), and PFGE profile (data not shown). Thus, although a potential food or animal origin has been proposed for certain human-associated, multidrug-resistant uropathogenic E. coli clonal groups (26,38), and a close relationship has been demonstrated between E. coli from retail chicken meat and E. coli causing human UTI (52), no candidate animal source for the Copenhagen outbreak was identified here.…”

Section: Discussionmentioning

confidence: 92%

Enteroaggregative Escherichia coli O78:H10, the Cause of an Outbreak of Urinary Tract Infection

Olesen

Scheutz

Andersen³

et al. 2012

J Clin Microbiol

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eIn 1991, multiresistant Escherichia coli O78:H10 strains caused an outbreak of urinary tract infections in Copenhagen, Denmark. The phylogenetic origin, clonal background, and virulence characteristics of the outbreak isolates, and their relationship to nonoutbreak O78:H10 strains according to these traits and resistance profiles, are unknown. Accordingly, we extensively characterized 51 archived E. coli O78:H10 isolates (48 human isolates from seven countries, including 19 Copenhagen outbreak isolates, and 1 each of calf, avian, and unknown-source isolates), collected from 1956 through 2000. E. coli O78:H10 was clonally heterogeneous, comprising one dominant clonal group (61% of isolates, including all 19 outbreak isolates) from ST10 (phylogenetic group A) plus several minor clonal groups (phylogenetic groups A and D). All ST10 isolates, versus 25% of non-ST10 isolates, were identified by molecular methods as enteroaggregative E. coli (EAEC) (P < 0.001). Genes present in >90% of outbreak isolates included fimH (type 1 fimbriae; ubiquitous in E. coli); fyuA, traT, and iutA (associated with extraintestinal pathogenic E. coli [ExPEC]); and sat, pic, aatA, aggR, aggA, ORF61, aaiC, aap, and ORF3 (associated with EAEC). An outbreak isolate was lethal in a murine subcutaneous sepsis model and exhibited characteristic EAEC "stacked brick" adherence to cultured epithelial cells. Thus, the 1991 Copenhagen outbreak was caused by a tight, non-animal-associated subset within a broadly disseminated O78:H10 clonal group (ST10; phylogenetic group A), members of which exhibit both ExPEC and EAEC characteristics, whereas O78:H10 isolates overall are phylogenetically diverse. Whether ST10 O78:H10 EAEC strains are both uropathogenic and diarrheagenic warrants further investigation.

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Section: Discussionmentioning

confidence: 92%

Enteroaggregative Escherichia coli O78:H10, the Cause of an Outbreak of Urinary Tract Infection

Olesen

Scheutz

Andersen³

et al. 2012

J Clin Microbiol

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“…In recent years, ESBLproducing Enterobacteriaceae isolates have shifted from the hospital to the community and the environment [41]. ESBL-producing Enterobacteriaceae have been recovered from different sources in the community, including cattle, chickens, pigs, raw milk and lettuce [42][43][44]. Recent study from India reported that a substantial number of tap water samples were contaminated with carbapenemase blaNDM-1 producing organisms [45].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Badri¹,

Ibrahim²,

Mohamed³

et al. 2017

Mol Biol

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Milk play a major role in human sources of nutrition and remain as the most important prominent in the Sudanese diet. Escherichia coli and Klebsiella pneumoniae are humans and animals opportunistic pathogens, responsible for a wide range of infections. The aim of this study was to evaluate the quality of the commercial available milk and to detect ESBL producing E. coli and K. pneumoniae from raw milk samples of cow in Al Jazirah state, Sudan. Seventy fresh row cow milk samples were collected and examined using standard microbiological methods, ESBL detection was performed on all the isolates by Ceftazidime screening test, those shows positive results by screening method were subjected to ESBL confirmatory test using Double-Disk Synergy Test and Molecular base detection using conventional PCR. Out of the 70 collected samples, 58 (82.8%) showed positive isolating result, the highest prevalence of the isolates was K. pneumoniae 36 (62%) followed by E. coli 22 (38%). The most resistance antibiotics against isolates was Ampicillin (98%), ESBL production was detected among 17 out of the 22 isolated E. coli (29.3%) and 26 (44.8%) out of the 36 isolated K. pneumoniae. The ESBL gene encoding the ESBL isolates was CTX-M gene representing 61% fellows by SHV gene (23%) and TEM gene (16%). ESBL-producing bacteria may also be transferred via waste milk to calves, thus further spreading antibiotic resistance in the farm environment.

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“…Indeed, to our knowledge, E. coli O15:K52:H1 has never been isolated from either animals or meat products, although this could simply reflect limited sampling. A potential animal origin of certain human-associated multidrug-resistant uropathogenic E. coli strains has been proposed (18,25) but remains unproven. The absence of animal isolates in the present population argues against a food animal reservoir for E. coli O15:K52:H1.…”

Section: Discussionmentioning

confidence: 99%

“…The study was limited to (i) Danish isolates, regardless of previous publication status, and (ii) previously unpublished isolates from other countries, using one isolate per patient. Seven English, eight Spanish, and three American O15:K52:H1 isolates were excluded by virtue of previous publication (8,24,25), six isolates were duplicates from the same patients (all Danish), and one was subsequently lost (total number excluded, 25). Thus, 100 isolates from 100 different patients infected with E. coli O15:K52:[H1] from 1975 to 2006 were studied.…”

Section: Methodsmentioning

confidence: 99%

Three-Decade Epidemiological Analysis of Escherichia coli O15:K52:H1

et al. 2009

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The successful Escherichia coli O15:K52:H1 clonal group provides a case study for the emergence of multiresistant clonal groups of Enterobacteriaceae generally. Accordingly, we tested the hypotheses that, over time, the O15:K52:H1 clonal group has become increasingly (i) virulent and (ii) resistant to antibiotics. One hundred archived international E. coli O15:K52:[H1] clinical isolates from 100 unique patients (1975 to 2006) were characterized for diverse phenotypic and molecular traits. All 100 isolates derived from phylogenetic group D and, presumptively, sequence type ST393. They uniformly carried the F16 papA allele and papG allele II (P fimbria structural subunit and adhesin variants), iha (adhesin-siderophore), fimH (type 1 fimbriae), fyuA (yersiniabactin receptor), iutA (aerobactin receptor), and kpsM II (group 2 capsule); 85% to 89% of them contained a complete copy of the pap operon and ompT (outer membrane protease). Slight additional virulence profile variation was evident, particularly within a minor diarrhea-associated subset (biotype C). However, in contrast to the clonal group's fairly stable virulence profiles over the past 30؉ years, during the same interval the clonal group members' antimicrobial resistance profiles increased by a mean of 2.8 units per decade (P < 0.001). Moreover, the numbers of virulence genes and resistance markers were positively associated (P ‫؍‬ 0.046), providing evidence against antimicrobial resistance and virulence being mutually exclusive in these strains. Thus, the O15:K52:H1 clonal group has become increasingly resistant to antimicrobials while maintaining (or expanding) its virulence potential, a particularly concerning trend if other emerging multiresistant enterobacterial clonal groups follow a similar pattern.Escherichia coli strains of serotype O15:K52:H1 first came to clinicians' attention as significant pathogens in 1986 and 1987, during a year-long, community-wide outbreak of multiresistant infections in South London (23). The outbreak strain exhibited a 100-MDa plasmid and the signature resistance profile ACSSuTTp (resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides, tetracycline, and trimethoprim). Most patients had urinary tract infections, but some had septicemia associated with pneumonia, meningitis, or endocarditis, and three died. E. coli O15:K52:H1 was later documented as a significant extraintestinal pathogen elsewhere in Europe (19,24) and also shown to be distributed widely outside Europe, where it exhibited previously unrecognized phenotypic and genotypic diversity (8). Subsequently, rapid and specific detection of the O15:K52:H1 clonal group by single-nucleotide polymorphism (SNP)-specific PCR was described (12). In a recent Italian study, the O15:K52:H1 clonal group accounted for 16 (11%) of 148 ciprofloxacin-resistant E. coli isolates from uncomplicated urinary tract infections in eight European countries (2003 to 2006), providing evidence of continued clinical importance and a seemingly new resistance phenotype for this clon...

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Possible Animal Origin of Human-Associated, Multidrug-Resistant, Uropathogenic Escherichia coli

Abstract: These observations suggest that drug-resistant, uropathogenic human-associated E. coli strains potentially have an animal origin. The possibility that human drug-resistant UTI could be a foodborne illness has serious public health implications.

Cited by 140 publications

References 26 publications

Enteroaggregative Escherichia coli O78:H10, the Cause of an Outbreak of Urinary Tract Infection

Enteroaggregative Escherichia coli O78:H10, the Cause of an Outbreak of Urinary Tract Infection

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Three-Decade Epidemiological Analysis of Escherichia coli O15:K52:H1

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