Positively-Charged, Porous, Polysaccharide Nanoparticles Loaded with Anionic Molecules Behave as ‘Stealth’ Cationic Nanocarriers

Paillard, Archibald; Passirani, Catherine; Saulnier, Patrick; Kroubi, Maya; Garcion, Emmanuel; Benoı̂t, Jean-Pierre; Betbeder, Didier

doi:10.1007/s11095-009-9986-z

Cited by 49 publications

(53 citation statements)

References 48 publications

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“…Zeta potential decreased to −2.22 ± 0.86 mV after polysorbate 80 was applied. The surface charge of bovine serum albumin is −5.6 mV, according to Paillard et al, and the potential of NPs decreases after adsorption on the surface of NPs [34]. Therefore, in this study, the potential reduction of NPs in 0.7 mmol polysorbate 80 emulsifier may also be related to the adsorption.…”

Section: Characterization Of Chp Dzp-chp and Ps-dzp-chpsupporting

confidence: 50%

Preparation and Drug Release Study of Novel Nanopharmaceuticals with Polysorbate 80 Surface Adsorption

Tao

et al. 2018

Journal of Nanomaterials

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Most free drugs that cross the blood-brain barrier are characterized by high liposolubility, but they often have limited clinical applications because of poor dissolution and poor bioavailability. In this study, we prepared donepezil drug-loaded nanoparticles (DZP) with cholesterol-modified pullulan (CHP) as the nanocarrier (DZP-CHP) and surface modified the drugloaded nanoparticles to improve the water solubility of donepezil while enhancing its targeting and sustained release. We determined the drug loading and encapsulation efficiency of DZP-CHP nanoparticles at different feed ratios. The mean ± SD drug loading and entrapment efficiency were high: 13.52 ± 2.03 and 86.54 ± 1.31. On dynamic light-scattering measurement, mean ± SD particle size was 260.7 ± 1.76 nm, polydispersity index 0.123 ± 0.004, and zeta potential −5.75 ± 0.64 mV. DZP-CHP nanoparticles prepared with the optimal feed ratio (DZP : CHP = 1 : 5) were coated with polysorbate 80, and the adsorption process was determined by isothermal titration calorimetry. We found good affinity between polysorbate 80 and DZP-CHP, with mean ± SD coverage 2.7 ± 0.372. The mean ± SD drug loading and entrapment efficiency of polysorbate 80-emulsified DZP-CHP nanoparticles were 8.25 ± 1.80 and 91.28 ± 4.57, respectively, and the proportion of drug released by 72 h was 42.71%. Compared to DZP-CHP alone, PS-DZP-CHP can enhance the release of donepezil.

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Section: Characterization Of Chp Dzp-chp and Ps-dzp-chpsupporting

confidence: 50%

Preparation and Drug Release Study of Novel Nanopharmaceuticals with Polysorbate 80 Surface Adsorption

Tao

et al. 2018

Journal of Nanomaterials

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“…Porous maltodextrin-based with lipid core nanoparticles (DGNP) were prepared as described previously [37]. Briefly, maltodextrin were dissolved in 2 N sodium hydroxide with magnetic stirring at room temperature.…”

Section: Dgnp Synthesismentioning

confidence: 99%

“…DGNP are porous nanoparticles (NPs) prepared from maltodextrin as previously described [37] with anionic phospholipids in their core (Fig. 1).…”

Section: Dgnp Characterizationmentioning

confidence: 99%

“…BSA was loaded by mixing premade nanoparticles and proteins at room temperature [37]. To test the loading capacity of DGNP, various amounts of nanoparticles (from 2 mg to 40 mg) were mixed with 4 mg of BSA and the resulting formulations (DGNP/BSA mass ratio from 0.5 to 10) were analyzed by polyacrylamide gel electrophoresis under non-denaturing conditions (native PAGE) to allow the electrophoretic migration of unbound proteins from the formulations.…”

Section: Loading Of Dgnp With Proteinsmentioning

confidence: 99%

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Porous nanoparticles as delivery system of complex antigens for an effective vaccine against acute and chronic Toxoplasma gondii infection

et al. 2015

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“…Stealth nanoparticles are generally obtained after modifying their surface with hydrophilic polymers, such as PEG. In this study, the effect of a phospholipid (DG) or protein (BSA) inclusion in porous cationic polysaccharide (NP (+)) on their physico-chemical structure and the effect on complement activation was analyzed [27]. Unfolded protein response (UPR) has evolved to counter the stresses that occur in the endoplasmic reticulum (ER) as a result of misfolded proteins.…”

Section: Protein Structurementioning

confidence: 99%

Protein Based Drug Discovery

Joshi¹,

Boraste²,

Khairnar³

et al. 2009

Int J of Drug Disc

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Abstract-New drug target discovery is currently very popular with a great potential for advancing biomedical research and chemical genomics. Drug discovery is the process of discovering and designing drugs that includes target identification, target validation, lead identification, lead optimization and introduction of the new drugs to the public. G protein-coupled receptors are one of the most important drug targets. In the current scenario of drug research, approximately 60% of drug target molecules are located at the cell surface, and half of them are GPCRs. Fragment-based drug discovery is established as an alternative approach to high-throughput screening for generating novel small molecule drug candidates. Nanotechnology-based drug delivery systems have seen recent popularity due to their favorable physical, chemical, and biological properties, and great efforts have been made to target nanoDDSs to specific cellular receptors. Protein-protein interactions regulate a wide variety of important cellular pathways, and therefore represent a highly populated class of targets for drug discovery. An analysis of individual proteinprotein interaction systems has recently yielded success in the discovery of drug-like inhibitors.

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Positively-Charged, Porous, Polysaccharide Nanoparticles Loaded with Anionic Molecules Behave as ‘Stealth’ Cationic Nanocarriers

Abstract: These nanoparticles are able to absorb large amounts of phospholipids or proteins without change in their size or zeta potential. Complement studies showed that stealth behaviour is observed when they are loaded and saturated either with anionic phospholipid or proteins.

Cited by 49 publications

References 48 publications

Preparation and Drug Release Study of Novel Nanopharmaceuticals with Polysorbate 80 Surface Adsorption

Preparation and Drug Release Study of Novel Nanopharmaceuticals with Polysorbate 80 Surface Adsorption

Porous nanoparticles as delivery system of complex antigens for an effective vaccine against acute and chronic Toxoplasma gondii infection

Protein Based Drug Discovery

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