Positively charged bioactive Ti metal prepared by simple chemical and heat treatments

Kokubo, Tadashi; Pattanayak, Deepak K.; Yamaguchi, Seiji; Takadama, Hiroaki; Matsushita, Tomiharu; Kawai, Toshiyuki; Takemoto, Mitsuru; Fujibayashi, Shunsuke; Nakamura, Takashi

doi:10.1098/rsif.2010.0129.focus

Cited by 108 publications

(105 citation statements)

References 28 publications

(37 reference statements)

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“…The result is similar to other studies 19,20) . While for the anodized Ti samples, a new layer of nanocrystals with a fine structure grew uniformly on the TiO 2 nanotubes (Figure2b).…”

Section: Simulated Body Fluid Soaking Testsupporting

confidence: 93%

Anodic Oxidation Modification Improve Bioactivity and Biocompatibility of Titanium Implant Surface

Liu

et al. 2013

J. Hard Tissue Biology.

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Current dental implant research aims at seeking an innovative surface able to promote a more favorable biological response to the cells and tissues at the bone-implant interface and to accelerate osseointegration. Anodic oxidation is a promising method for acquiring nanotube structures on the implant surface. In this paper, we modified the titanium surface using anodic oxidation to form nanotube structures on the surface and observed the surface by scanning electron microscopy (SEM). The bioactivity of the Ti implants was evaluated by simulated body fluid soaking test. We further sought to characterize the cellular and molecular responses of murine preosteoblast MC3T3-E1 cells to anodic oxidation modified titanium surface with a nanotube-texture. Gene expression of osteoprotegrin (OPG) was also evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR. SEM showed that the inner diameter of the nanotubes was about 70nm, the wall thickness was around 20nm, and the depth was about 200nm. The simulated body fluid soaking test displayed that bone-like apatite was formed on the nanotube-textured Ti surface after immersion in simulated body fluid for two weeks, but not on the smooth surface Ti surface. The biocompatibility was investigated by an in vitro cell culture test. Cell morphology exhibited a more differentiated characteristic, and gene expression of osteogenic markers OPG was also remarkably upregulated by anodic oxidation modification. Based on these results, it can be concluded that bioactivity and osteogenic responses to the nanotube-structured Ti surface were better than to the smooth surface, and gene expression indicates that OPG activation may be responsible for this increased osteogenic differentiation.

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“…The result is similar to other studies 19,20) . While for the anodized Ti samples, a new layer of nanocrystals with a fine structure grew uniformly on the TiO 2 nanotubes (Figure2b).…”

Section: Simulated Body Fluid Soaking Testsupporting

confidence: 93%

Anodic Oxidation Modification Improve Bioactivity and Biocompatibility of Titanium Implant Surface

Liu

et al. 2013

J. Hard Tissue Biology.

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“…The positive effects of bioactive treatments have been mainly attributed to the altered surface chemistry and charge, and subsequent apatite formation on the material in vivo and in a simulated body fluid 13,43,45) . In this in vitro study, we might assume that the nanoscale pores and increased wettability of the SLM-T material might also have had beneficial effects on osteoblast differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Examples include hydroxyapatite coating 9) and peptide immobilization 10) . Of these, bioactive treatments refer to surface treatments that promote direct bone-implant bonding by simple chemical and thermal procedures [11][12][13] . Orthopedic implants treated with a bioactive treatment are already available 14) .…”

Section: Introductionmentioning

confidence: 99%

Bioactive treatment promotes osteoblast differentiation on titanium materials fabricated by selective laser melting technology

et al. 2016

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Selective laser melting (SLM) technology is useful for the fabrication of porous titanium implants with complex shapes and structures. The materials fabricated by SLM characteristically have a very rough surface (average surface roughness, Ra=24.58 µm). In this study, we evaluated morphologically and biochemically the specific effects of this very rough surface and the additional effects of a bioactive treatment on osteoblast proliferation and differentiation. Flat-rolled titanium materials (Ra=1.02 µm) were used as the controls. On the treated materials fabricated by SLM, we observed enhanced osteoblast differentiation compared with the flat-rolled materials and the untreated materials fabricated by SLM. No significant differences were observed between the flat-rolled materials and the untreated materials fabricated by SLM in their effects on osteoblast differentiation. We concluded that the very rough surface fabricated by SLM had to undergo a bioactive treatment to obtain a positive effect on osteoblast differentiation.

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“…As the phosphate ions began to accumulate, the surface became negatively charged and combined with the positively charged calcium ions to form calcium phosphate. Calcium phosphate eventually transformed into stable crystalline apatite 33) . This mechanism well explained the improved apatite formation ability on MAO surface after UV irradiation.…”

Section: Discussionmentioning

confidence: 99%

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

Huang

et al. 2015

Dent. Mater. J.

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This present study investigated the effect of ultraviolet (UV) irradiation on bioactivity of micro-arc oxidized (MAO) titanium surface in vitro by cell culture medium immersion test and interactions with rat-derived mesenchymal stem cells (MSCs). UV-irradiated MAO surface exhibited no obvious changes in surface roughness, morphology, and phase composition when compared with MAOonly surface. However, in cell culture medium immersion test, markedly more bone-like apatite was formed on UV-modified samples than on MAO sample. Rat bone marrow-and adipose tissue-derived MSCs cultured on UV-modified samples displayed accelerated attachment, significant higher levels of alkaline phosphatase (ALP) activity, and up-regulated osteogenesis-related mRNA expression than MAO sample. XPS results provided direct evidence that the amount of basic hydroxyl groups increased with UV irradiation time, which could be one of the key mechanisms underlying their improved bioactivity.

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Positively charged bioactive Ti metal prepared by simple chemical and heat treatments

Cited by 108 publications

References 28 publications

Anodic Oxidation Modification Improve Bioactivity and Biocompatibility of Titanium Implant Surface

Anodic Oxidation Modification Improve Bioactivity and Biocompatibility of Titanium Implant Surface

Bioactive treatment promotes osteoblast differentiation on titanium materials fabricated by selective laser melting technology

Ultraviolet irradiation enhanced bioactivity and biological response of mesenchymal stem cells on micro-arc oxidized titanium surfaces

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