Porphyrin functionalized graphene nanosheets-based electrochemical aptasensor for label-free ATP detection

Abstract

A facile strategy for the preparation of meso-terakis(4-methoxyl-3-sulfonatophenyl) porphyrin (T(4-Mop)PS 4 )-graphene hybrid nanosheets (TGHNs) was demonstrated for the first time, which combines the features of both graphene (high conductivity and large specific surface area) and porphyrins (photochemical electron-transfer ability and excellent electrochemical activity). The as-obtained TGHNs were characterized by UV-vis spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, atomic force microscopy, transmission electron microscopy and cyclic voltammetry, which confirmed that the porphyrin had been effectively functionalized on the surface of graphene. Combined with the high affinity and specificity of an aptamer, a simple, rapid, sensitive and label-free electrochemical aptasensor was successfully fabricated for adenosine triphosphate (ATP) detection. The proposed TGHN-based aptasensor achieved the direct electron transfer of porphyrin and showed good affinity and specificity towards ATP, which avoided interference such as the introduction of labeled-substances and the [Fe(CN) 6 ] 3À/4À probe. The preferable linear range for ATP was from 2.2 nM to 1.3 mM (R ¼ 0.9982) with a detection limit of 0.7 nM.

Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide

“…Reduced graphene oxide (RGO) was used in order to immobilize CoTCPP through strong π-π interactions [27–30], and to increase the specific surface area of GC electrodes. First of all, various quantities of RGO were drop-casted on electrodes, and the electrochemical capacitance measured by cyclic voltammetry (Figure 2A).…”

“…Porphyrins can irreversibly adsorb through π-π interactions on graphite [24], carbon nanotubes [25,26] and graphene [27–30]. Following this approach, we modified reduced graphene oxide (RGO) with CoTCPP, which we deposited on glassy carbon (GC) electrodes afterwards.…”

Investigation of the adsorption mechanism and preconcentration of sulfonamides using a porphyrin-functionalized Fe 3 O 4 -graphene oxide nanocomposite

“…In addition, Han et al [23] and Lv et al [24] also prepared porphyrin-functionalized graphene-based materials for the electrochemical detection of dopamine. Wu et al [25] and Zhang et al [26] used porphyrin-functionalized graphene coupled with an electrochemiluminescence technique and an electrochemical aptasensor for the ultrasensitive label-free detection of human telomerase activity and ATP, respectively. Additionally, Feng et al [27] developed a reusable graphene-based electrochemical aptasensor for label-free cancer cell detection.…”

Graphene for Detection of Adenosine Triphosphate, Nicotinamide Adenine Dinucleotide, Other Molecules, Gas, and Ions

“…This strategy provided some new avenues on the design of nanomaterial-based aptamer sensors to direct the development of nanoelectroanalytical chemistry. Another wisely designed electrochemical aptasensor for ATP detection was recently realized by using porphyrin-functionalized graphene nanosheets as the platform [3]. The ATP aptamer adsorption on the platform decreased the electrochemical signal of porphyrin.…”