Electrocatalytic miRNA Detection Using Cobalt  Porphyrin-Modified Reduced Graphene Oxide

Souza, Camille De; Zrig, Samia; Wang, Dengjun; Pham, Minh‐Chau; Piro, Benoı̂t

doi:10.3390/s140609984

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…In our study, the preparation of disposable CA‐GO‐PGEs was resulted only in 15 min. In this aspect, this approach was found less time‐consuming assay contrast to the earlier studies performed by glassy carbon electrodes , or screen‐printed gold electrode . Additionally, the detection of miRNA achieved in both buffer (PBS) and diluted FBS medium within 35 min respectively with a detection limit of 0.29 μg/mL (i.e, 41.2 nM), and 1.12 μg/mL (i.e., 159 nM).…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

et al. 2017

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In our study, graphene oxide (GO) modified graphite electrodes were used for sensitive and selective impedimetric detection of miRNA. After chemical activation of pencil graphite electrode (PGE) surface using covalent agents (CA), GO modification was performed at the surface of chemically activated PGE. Then, CA‐GO‐PGEs were applied for impedimetric miRNA detection. The microscopic and electrochemical characterization of CA‐GO‐PGEs was performed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The optimization of experimental conditions; such as GO concentration, DNA probe concentration and miRNA target concentration was performed by using EIS technique. After the hybridization occurred between miRNA‐34a RNA target and its complementary DNA probe, the hybrid was immobilized onto the surface of CA‐GO‐PGEs. Then, the impedimetric detection of miRNA‐DNA hybridization was performed by EIS. The selectivity of our assay was also tested under the optimum experimental conditions.

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Section: Resultsmentioning

confidence: 99%

Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

et al. 2017

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“…In comparison to the previous reports, our proposed electrochemical biosensor platform presented some advantages; such as, easy-to-handle, cost-effective, fast, disposable electrode surface modification and portable. In this aspect, our biosensor provides a cost-effective assay in contrast to the earlier studies performed using screen-printed electrodes [ 41 ], or gold electrodes [ 42 ], AuNPs-modified electrodes [ 43 ] and some optical biosensor systems [ 44 ]. Additionally, the detection of miRNA achieved in both buffer (PBS) and diluted FBS medium within detection limits of 109 nM and 117 nM, respectively.…”

Section: Discussionmentioning

confidence: 99%

An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

Kesici

Ekşin

Erdem

2018

Sensors

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In the present work, an impedimetric nucleic acid biosensor has been designed for the purpose of detection of microRNA (miRNA). Ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL))-modified chemically activated pencil graphite electrodes (PGEs) were used for the sensitive and selective detection of miRNA-34a. After covalent activation of the PGE surface using covalent agents (CAs), the ionic liquid (IL) was immobilized onto the surface of the chemically activated PGE by passive adsorption. The electrochemical and microscopic characterization of the IL/CA/PGEs was performed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and scanning electron microscopy (SEM). DNA probe concentration, miRNA target concentration, and also the hybridization time and wet adsorption time were optimized by using the EIS technique. Then, the hybridization occurred between specific DNA probes and miRNA-34a was immobilized onto the surface of the IL/CA/PGEs. The impedimetric detection of miRNA-DNA hybrid was performed by EIS. The detection limit (DL) was calculated in a linear concentration range of 2–10 µg/mL miRNA-34a target, and it was found to be 0.772 µg/mL (109 nM) in phosphate buffer solution (PBS) and 0.826 µg/mL (117 nM) in diluted fetal bovine serum (FBS). The selectivity of impedimetric biosensor for miRNA-34a was also tested against to other non-complementary miRNA sequences both in buffer media, or diluted FBS.

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“…Generally, its rapid detection relies on classical techniques such as Northern blot, real-time quantitative reverse transcription PCR (qRT-PCR), or optical DNA chips based SPR or fluorescence labeling. De Souza et al reported a carboxylic acid functionalized cobalt porphyrin (CoTCPP, cobalt tetra(4-carboxyphenyl) porphyrin)) noncovalently physisorbed (via π–π interactions) on reduced graphene oxide (Figure A) and then immobilized on a glassy carbon electrode . Further, the carboxylic groups were covalently grafted with amino-terminated oligonucleotide probes, complementary to a short microRNA target.…”

Section: Porphyrin and Graphene/carbon Nanotube Conjugatesmentioning

confidence: 99%

Organic–Inorganic Porphyrinoid Frameworks for Biomolecule Sensing

et al. 2023

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Porphyrinoids and their analogous compounds play an important role in biosensing applications on account of their unique and versatile catalytic, coordination, photophysical, and electrochemical properties. Their remarkable arrays of properties can be finely tuned by synthetically modifying the porphyrinoid ring and varying the various structural parameters such as peripheral functionalization, metal coordination, and covalent or physical conjugation with other organic or inorganic scaffolds such as nanoparticles, metal–organic frameworks, and polymers. Porphyrinoids and their organic–inorganic conjugates are not only used as responsive materials but also utilized for the immobilization and embedding of biomolecules for applications in wearable devices, fast sensing devices, and other functional materials. The present review delineates the impact of different porphyrinoid conjugates on their physicochemical properties and their specificity as biosensors in a range of applications. The newest porphyrinoid types and their synthesis, modification, and functionalization are presented along with their advantages and performance improvements.

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Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide

Cited by 10 publications

References 31 publications

Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection

Organic–Inorganic Porphyrinoid Frameworks for Biomolecule Sensing

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