Porous Poly(vinyl alcohol)-Alginate Gel Hybrid Construct for Neocartilage Formation Using Human Nasoseptal Cells

Bichara, David A.; Zhao, Xing; Hwang, Nathaniel S.; Bodugoz-Senturk, Hatice; Yaremchuk, Michael J.; Randolph, Mark A.; Muratoglu, Orhun K.

doi:10.1016/j.jss.2010.03.070

Cited by 64 publications

(47 citation statements)

References 26 publications

(24 reference statements)

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“…Mechanical evaluation has often been overlooked in AUR cartilage TE attempts. Many authors (Arevalo-Silva et al, 2000;Cao et al, 1998Cao et al, , 1997Haisch et al, 2002;Isogai et al, 2004Isogai et al, , 2008Kamil et al, 2004Kamil et al, , 2003Kusuhara et al, 2009;Liu et al, 2010;Neumeister et al, 2006;Ruszymah et al, 2011;Saim et al, 2000;Shieh et al, 2004;Xu et al, 2005) report a qualitative mechanical assessment, while a few publications report quantitative data but without comparison to human AUR cartilage (Bichara et al, 2010;Britt and Park, 1998;Chang et al, 2003;Isogai et al, 2005;Ting et al, 1998). Indentation has been shown previously to be a good and sensitive first approximation for direct comparison between native and tissue-engineered constructs (Duda et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs

Nimeskern

Pleumeekers

Pawson

et al. 2015

Journal of Biomechanics

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Section: Introductionmentioning

confidence: 99%

Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs

Nimeskern

Pleumeekers

Pawson

et al. 2015

Journal of Biomechanics

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“…In vivo results showed the sulfated glycosaminoglycans and deposition of collagen type II in hydrogels from both groups. Second group system showed higher level of DNA, glycosaminoglycans and hydroxyproline and an increase of 22% in the compressive strength[143]. Chhatriet al 2011 prepared savlon-loaded PVA/Alg macroporous hydrogels which showed clear inhibition-zones around the loaded composite hydrogels providing fair antimicrobial activity and offered extremely fair in vitro blood compatibility[144].…”

mentioning

confidence: 99%

PVA-based hydrogels for tissue engineering: A review

Kumar

Han

2016

International Journal of Polymeric Materials and Polymeric Biom

364

161

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Poly (vinyl alcohol) (PVA) is a hydrophilic polymer with excellent biocompatibility and has been applied in various biomedical areas due to its favorable properties. PVA-based hydrogels have been recognized as promising biomaterials and suitable candidate for tissue engineering applications and can be manipulated to act various critical roles.However, due to some disadvantages (i.e., lack of cell-adhesive property), it needs further modification for desired and targeted applications. This review highlights recent progress in the design and fabrication of PVA-based hydrogels, including cross-linking and processing techniques. Finally, major challenges and future perspectives in tissue engineering are briefly discussed. GRAPHICAL ABSTRACTDownloaded by [University of Sussex Library] at 08:36 28 June 2016 2

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“…13 8 injection of an alginate-chondrocyte mix in a porous poly(vinyl alcohol), PVA, implant. Six weeks after implantation in nude mice, neocartilage formation was reported with promising increases in mechanical strength (36). However, the suitability of the test for cartilage function is disputed and the comparison to native tissue is missing.…”

Section: B Auricular Cartilage Tissue-engineeringmentioning

confidence: 99%

Quantitative Evaluation of Mechanical Properties in Tissue-Engineered Auricular Cartilage

Nimeskern

Osch

Müller

et al. 2014

Tissue Engineering Part B: Reviews

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Keywords:Auricular cartilage, elastic cartilage, auricle, pinna, outer ear, mechanical testing, elastin Conflict of interest:All authors have no conflict of interest. 3 AbstractTissue-engineering (TE) efforts for ear-reconstruction often fail due to mechanical incompetency. It is therefore key for successful auricular cartilage TE to ensure functional competency, i.e. to mimic the mechanical properties of the native ear tissue. A review of past attempts to engineer auricular cartilage shows unsatisfactory functional outcomes with various cell-seeded biodegradable polymeric scaffolds in immunocompetent animal models. However, promising improvements to construct stability were reported with either mechanically-reinforced scaffolds or novel two-stage implantation techniques. Nonetheless, quantitative mechanical evaluation of the constructs is usually overlooked, and such an evaluation of TE constructs alongside a benchmark of native auricular cartilage would allow real-time monitoring and improve functional outcomes of auricular TE strategies.Although quantitative mechanical evaluation techniques are readily available for cartilage, these techniques are designed to characterize the main functional components of hyaline and fibrous cartilage such as the collagen matrix or the glycosaminoglycan (GAG) network, but they overlook the functional role of elastin, which is a major constituent of auricular cartilage. Hence for monitoring auricular cartilage TE, novel evaluation techniques need to be designed. These should include a characterization of the specific composition and architecture of auricular cartilage, as well as mechanical evaluation of all functional components. Therefore, this paper reviews the existing literature on auricular cartilage TE as well as cartilage mechanical evaluation and proposes recommendations for designing a mechanical evaluation protocol specific for auricular cartilage, and establishing a benchmark for native auricular cartilage to be used for quantitative evaluation of TE auricular cartilage.15.02.13 4

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Porous Poly(vinyl alcohol)-Alginate Gel Hybrid Construct for Neocartilage Formation Using Human Nasoseptal Cells

Cited by 64 publications

References 26 publications

Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs

Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs

PVA-based hydrogels for tissue engineering: A review

Quantitative Evaluation of Mechanical Properties in Tissue-Engineered Auricular Cartilage

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