Porcine peripheral blood CD4+/CD8+ dual expressing T-cells

Pescovitz, M.D; Sakopoulos, Andreas G.; Gaddy, Jay; Husmann, Robert J.; Zuckermann, Federico A.

doi:10.1016/0165-2427(94)90120-1

Cited by 111 publications

(60 citation statements)

References 28 publications

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“…9 The anti-CD3 mAb described here permit an unambiguous identification of porcine T cells and hence a more accurate classification of porcine lymphocytes. Thus, the 'null' cells (CD2-negative, surface immunoglobulin-negative) identified by mAb MAC320 are now shown to express CD3 antigen and hence are T cells.…”

Section: Discussionmentioning

confidence: 97%

Preparation of monoclonal anti‐porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes

et al. 1996

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SUMMARYThe CD3-T-cell receptor complex is the clonotypic surface structure by which T lymphocytes recognize foreign antigens and are subsequently activated. Because of the low immunogenicity of the CD3 molecules, anti-CD3 monoclonal antibodies (mAb) are difficult to prepare and have not been available in several species. Following isolation of porcine CD3, 14 anti-porcine CD3 mAb were prepared, which define six groups of CD3-e epitopes, coprecipitate two types of TCR and reveal considerable heterogeneity of CD3 expression amongst lymphocyte subpopulations. Thus, both CD3 positive and negative subpopulations of CD2 or CD8 positive cells were found in the blood. The density of CD3 on CD2 or CD8 cells was relatively low and heterogeneous, whereas the CD2 ÿ , CD8 ÿ or MAC320 T cells expressed CD3 at a higher and more homogeneous level. Finally, in the thymus, staining with anti-CD3 resolved large thymocytes into two subsets: one expressing a high level of CD3 and the other being negative. In contrast, small thymocytes expressed CD3 at a low and more homogeneous level. Immunohistological studies confirmed the presence of clearly detectable CD3 in thymus medulla and the T-cell regions of peripheral lymphoid tissues. Most of the mAb were mitogenic, when presented to peripheral blood mononuclear cells in immobilized form. The anti-CD3 mAb also induced redirected cytotoxicity which was shown to be Fc receptor dependent.

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Section: Discussionmentioning

confidence: 97%

Preparation of monoclonal anti‐porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes

et al. 1996

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“…1 For example, in addition to the presence in the peripheral blood of normal adult pigs of the typical CD2 T-cell subsets expressing either CD4 (characteristic of T helper cells) or CD8 (characteristic of cytotoxic/suppressor T lymphocytes), there is a sizeable population (10-60%) of CD2 lymphocytes which are CD4/CD8 double-positive (DP). [2][3][4][5] While circulating CD4/CD8 DP T lymphocytes are present only in small proportions in human peripheral blood (<3%), 6,7 this T-cell subset has been found in greater proportions (>15%) in the peripheral blood of patients suffering from neoplasia, 8 in the joint fluid of rheumatoid arthritis patients, 9 and among lymphocytes infiltrating human 10 and swine 11 kidney allografts. In addition CD4/CD8 DP cells are well represented among intestinal intraepithelial lymphocytes (iIEL) of both mice and rats.…”

Section: Introductionmentioning

confidence: 99%

Distribution of porcine CD4/CD8 double‐positive T lymphocytes in mucosa‐associated lymphoid tissues

Zuckermann¹,

Gaskins

1996

Immunology

145

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SUMMARYThe present study describes the distribution of CD4/CD8 double-positive (DP) T cells in lymph nodes and mucosa-associated lymphoid tissues of pigs at 6-7 months of age. Proportions of lymphocytes isolated from peripheral, bronchial and mesenteric lymph nodes expressing CD4 and/or CD8 molecules were similar and ranged from 10-13% CD4/CD8 DP cells, 25-27% CD4 single-positive (SP) cells, and 27-32% CD8 SP cells. Mucosa-associated lymphoid tissues had significantly smaller proportions of CD4 and/or CD8 T cells than lymph nodes and CD4/CD8 DP cells accounted for a larger proportion of the total CD4 lymphocytes than in lymph nodes. Compared to the lymph nodes, the predominant CD4and/or CD8 T-cell subset in tonsils was the CD4/CD8 DP population (18%), because of both a higher proportion of these cells and a lower proportion of CD4 SP (12%) and CD8 SP (14%) lymphocyte subsets. Jejunal Peyer's patches were comprised of 12% CD4 SP, 28% CD8 SP and 10% CD4/CD8 DP lymphocytes. In contrast, the mid-section of the continuous Peyer's patch in the ileum contained 7% CD4 SP, 8% CD8 SP and 4% CD4/CD8 DP lymphocytes. The distribution of T lymphocytes in porcine mucosal lymphoid aggregates agrees with the reported correlation between high and low rates of lymphocyte entry into these tissues and the abundance or scarcity of T cells, respectively. Defining the role of CD4/CD8 DP lymphocytes and their unique distribution in mucosa-associated lymphoid tissues in the pig may reveal novel T-cell-mediated regulatory pathways.

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“…It increased the frequency of the CD3 + CD4 + CD8 + lymphocyte subset at 4 months. In pigs, these cells are known to be a subset of memory T cells (Pescovitz et al, 1994;Zuckermann and Husmann, 1996). The increase in these memory cells unlikely results from a greater immune stimulation of the immune system of surgically castrated male pigs (see the discussion above).…”

Section: Discussionmentioning

confidence: 99%

Effects of neonatal surgical castration and immunocastration in male pigs on blood T lymphocytes and health markers

Leclercq

Prunier

Merlot

2014

Animal

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Surgical castration in pig husbandry is criticized for welfare reasons. Thus, it is necessary to evaluate alternative ways of rearing male pigs, such as entire or immunocastrated animals. Immunocastration is a vaccination directed against gonadotropin-releasing hormone (GnRH) to suppress the production of sexual hormones. This study aimed at investigating the effects of these two methods of castration in comparison with intact male pigs on blood T-lymphocyte subsets and function, the immunoglobulin (Ig) response to an influenza vaccine and health markers during sexual development. A total of 70 animals were allocated to three experimental groups: entire (E), surgically castrated at 5 to 6 days of age (SC), and immunized against GnRH at 3 and 4 months of age (IC). Blood samples were collected at 3, 4 and 5 months. At slaughter, global health status and body and spleen weights were measured. Results showed that SC male pigs had fewer blood lymphocytes than E pigs at 4 and 5 months (P < 0.05), whereas IC pigs did not differ significantly from E pigs. The percentages of CD3 + , CD3 + CD4 + and CD3 + CD8 + lymphocytes were not altered by treatment (P > 0.1). Compared with E pigs, the SC pigs had a higher percentage of CD3 + CD4 + CD8 + cells at 4 months, whereas the IC pigs had a higher percentage at 5 months (P < 0.05). Regarding γδT cells, SC pigs had a lower percentage than E pigs at 4 and 5 months (P < 0.05), whereas IC pigs did not differ significantly from E pigs at any age. However, there were no consequences on T-lymphocyte proliferation and total IgG or anti-influenza Ig. At slaughter, relative spleen weight was decreased in IC pigs, whereas pneumonia score was decreased in SC pigs relatively to E pigs. Overall, no clear functional consequences of either method on commercial pig immune abilities were demonstrated, but more investigations are required to ascertain this conclusion.

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Porcine peripheral blood CD4+/CD8+ dual expressing T-cells

Cited by 111 publications

References 28 publications

Preparation of monoclonal anti‐porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes

Preparation of monoclonal anti‐porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes

Distribution of porcine CD4/CD8 double‐positive T lymphocytes in mucosa‐associated lymphoid tissues

Effects of neonatal surgical castration and immunocastration in male pigs on blood T lymphocytes and health markers

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