Porcine Fetal Enamel Matrix Derivative Stimulates Proliferation But Not Differentiation of Pre‐Osteoblastic 2T9 Cells, Inhibits Proliferation and Stimulates Differentiation of Osteoblast‐Like MG63 Cells, and Increases Proliferation and Differentiation of Normal Human Osteoblast NHOst Cells

Abstract

EMD affects early states of osteoblastic maturation by stimulating proliferation, but as cells mature in the lineage, EMD enhances differentiation.

Periodontal regeneration in intrabony defects after application of enamel matrix proteins with guided tissue regeneration: An experimental study in dogs

“…Furthermore, they showed that only periodontal ligament cells in the early stage of wound healing react with EMP. It has been also reported that EMP stimulates cell proliferation and acts on the early stage at days 7 and 10 of osteoblast maturation (10,19). These previous studies support our results that EMP acts a signifi cant rate in the early stage of wound healing in the periodontal ligament cells.…”

“…Examination of randomly selected tissues characterized the effects of treatments with GTR (5,10,19). Within the limits of our knowledge, the effects of EMP at the early stage (at 1, 2, and 4 weeks) of periodontal tissue healing have not been previously investigated in vivo.…”

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

“…This is supported by the observation that OPG levels significantly increased when MG63 cells were treated with lower doses of Fraction C in comparison to rhAmelogenin. Our results show that the levels of both active and latent TGF-1 in the conditioned medium were increased by rhAmelogenin and Fraction C, confirming previous studies in which treatment with rhAmelogenin increased TGF-1 mRNA or protein levels [30,68]. The increase in TGF-1 may cause an indirect effect on cell proliferation, differentiation, or extracellular matrix and growth factor production [69,70].…”

“…In vitro studies indicate that EMD has a differential effect on osteoblast proliferation and differentiation depending on the maturation state of the cells, increasing cell numbers in less mature cells and increasing differentiation in more mature cells, including increased alkaline phosphatase activity, osteocalcin, bone sialoprotein, and mineralized nodule formation [30][31][32][33]. EMD treatment results in an increase in proliferation of other cell types as well [34][35][36][37].…”

Porcine enamel matrix derivative enhances trabecular bone regeneration during wound healing of injured rat femur

“…Concerning the biological effects of EMD on osteoblastic mesenchymal cells, EMD appeared to stimulate proliferation of pre-osteoblastic 2T9, OCT-1, PDL cells, and MC3T3-E1 cells and enhance differentiation of osteoblast-like osteosarcoma MG-63 cells (Lyngstadaas et al, 2001;Schwartz et al, 2000;Tokiyasu et al, 2000;Van der Pauw et al, 2000). For PDL fibroblasts, EMD stimulated their proliferation, increased the autocrine release of transforming growth factor-␤, interleukin-6 (IL-6) and platelet-derived growth factor BB, enhanced the alkaline phosphatase expression and increased rapid attachment and spreading of these cells on purified type I collagen Lyngstadaas et al, 2001; Van der Pauw et al, 2000).…”