Population structure in the endangered Blanca Cacerena bovine breed demonstrated by RAPD analyses.

Inference of admixture in the endangered Blanca Cacereña bovine breed by microsatellite analyses

“…Currently, the breed comprises just under five hundred breeding animals. Moreover, the genetic studies performed such as inbreeding and molecular analyses of genetic variability (Parejo et al, 1998(Parejo et al, , 2002, allowed us to design a coordinate Plan of Conservation for this breed in two preservation programs: 1) live animals (management programs of conservation in nucleus and in herds) and 2) biological materials (DNA, blood, semen, ovules, and frozen embryos). This conservation program was started with two priorities: (1) maintaining maximum genetic diversity, with (2) the minimum possible consanguinity increase per generation (Calero et al, 2000).…”

“…Currently, molecular monitoring (by analyses of 20 microsatellite loci) of the nucleus's offspring and some animals from the conservationist herds (1 male and 5 females each) allow us to design the mating between the most genetically distant animals according to the between-individual coancestry coefficients (f ij ) (Caballero and Toro, 2002) and the kinship distance (D k ) at individual level (Gutiérrez et al, 2005). Nevertheless, the little reproductive success existing in the breed and chromosome abnormality detected (X-fragile) in some animals (data not published) can be due to high inbreeding of this population (Parejo et al, 1998(Parejo et al, , 2002. With the aim of extending the genetic basis of the animals of the conservation nucleus, the authorities are interested in opening the recorded herd book to new animals from local farms with little or no genetic flow from the other herds.…”

Use of RAPD-PCR for genetic analyses on the native cattle breeds in Turkey

“…The RAPD technique has also been used for constructing phylogenic relationships in different animals such as; goat (1), horse (1), cattle (1,2,3,16), sheep (1,3,9,12), dog (19), buffalo (1), swine (12) and chicken (2,20,24). Although the RAPD technique has low reproducibility (24), this and earlier studies showed that it is efficient technique because of its simplicity, speed and low cost (2,20).…”

Assessment of genetic variability in Murrah, Bhadawari and Nili-Ravi buffalo breeds of India by RAPD markers

“…The RAPD-PCR technique is highly sensitive to minor alterations in the reaction conditions. The number, intensity of amplification and reproducibility of amplified DNA fragments depend on a variety of variables such as PCR cycling conditions (Yu and Pauls, 1992; Don et al, 1991) [12,3] , template and primer concentrations (Muralidharan and Wakeland, 1993) [4] , and the components of the PCR buffer (Ponce and Micol, 1992) [6] . It is important to provide optimum conditions for primers to ensure efficient and reproducible amplification from reaction to reaction.…”