Population‐specific criterion to distinguish killer cell immunoglobulin‐like receptor genotypes and haplotypes in a large Eastern Han population

Abstract

With the goal of improving the population‐specific criteria to distinguish KIR genotypes and haplotypes in the region, we examined the KIR gene and haplotype data of Eastern Han based on a large population and analyzed the component genes of centromeric (Cen) and telomeric (Tel) KIR haplotype segments, which may differ in the protection they provide in hematopoietic stem cell transplantation. Samples from 598 families and 2845 unrelated individuals of Eastern Han origin were collected between 2010 and 2017. Genotyping of 17 KIR genes was performed by PCR‐SSP and SSO methods. The results showed we obtained the KIR gene distribution of the Eastern Han population. The KIR gene frequencies (GF) in the present study are similar to those observed in other studies on Han but different from other populations. We observed a total of 56 different genotypes, including 1 AA and 55 group B genotypes. The high‐frequency KIR genotype profiles found in the present population were consistent with other studies on Han populations but different from those conducted on other populations. In the family panel, a total of 28 KIR haplotypes were identified in the segregation study. The majority of Eastern Han carried group A KIR gene motifs. Comparison of the frequencies of Cen and Tel KIR gene motifs shows that they differ from other populations. The study on the distribution of KIR genes in the population may aid in the development of a complementary population‐specific criterion to distinguish between KIR haplotypes and offer a research direction for further gene functional studies.

Expression of Killer Immunoglobulin Receptor Genes among HIV-Infected Individuals with Non-AIDS Comorbidities

“…Our results were similar to previously reported data in the region specifically among healthy Lebanese [ 48 , 57 ], Iranian [ 58 , 59 ], and Turkish adults [ 60 ]. Similar results were also reported in Southern Brazil [ 61 , 62 ] and Eastern Han populations in China [ 63 ]. Moreover, the AA genotype profile was the most frequent among our study participants similar to previously reported data in healthy unrelated individuals in Lebanon [ 57 ], Turkey [ 60 ], Iran [ 58 ], and Tunisia [ 64 ].…”

Dynamic mRNA expression of donor-derived activating KIR genes and their significant effects on clinical outcome after haematopoietic stem cell transplantation

“…Regarding the SSOP method, LABType ® SSO Kits (One Lambda) and the Luminex 200 system were used. The criteria for KIR genotype assignments have been described in a previous report [24].…”