Polyurethane scaffolds seeded with genetically engineered skeletal myoblasts: a promising tool to regenerate myocardial function

Blumenthal, Britta; Golsong, Peter; Poppe, Annika; Heilmann, Claudia; Beyersdorf, Friedhelm; Schlensak, Christian; Siepe, Matthias

doi:10.1055/s-0029-1191349

Cited by 4 publications

(14 citation statements)

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“…The plasmid was a generous gift from Kensuke Egashira (Kyushu University, Fukuoka, Japan). Effective transfection of the cells and overexpression of SDF-1 were confirmed in vitro by RT-PCR and ELISA as reported in our previous study [18]. Injected SkM-SDF-1 secreted around 18% more SDF than untransfected myoblasts.…”

Section: Transfection Of Primary Skeletal Myoblastssupporting

confidence: 82%

“…Previous studies suggested a scaffold-based application of transplanted cells to improve study outcome by avoiding cell loss due to injection, diffusion, or poor engraftment and by preventing injection-induced arrhythmias [18,24]. Unexpectedly, the scaffold-based application of SDF-1 expressing myoblasts to the infarcted heart did not further enhance the positive effects on systolic function observed in group Inj-SDF-1-SkM.…”

Section: Assessment Of Hemodynamic Parametersmentioning

confidence: 84%

“…Injected SkM-SDF-1 secreted around 18% more SDF than untransfected myoblasts. SkM-SDF-1 seeded on scaffolds secreted around 13% more SDF-1 than untransfected cells at the time of application [18].…”

Section: Transfection Of Primary Skeletal Myoblastsmentioning

confidence: 95%

“…Cell transfection was performed as described previously [18]. In brief, the plasmid pcDNA3-huSDF-1 was delivered into SkM using the liposome-based transfection reagent Metafectene TM Pro (Biontex, Munich, Germany).…”

Section: Transfection Of Primary Skeletal Myoblastsmentioning

confidence: 99%

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Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application

Blumenthal

Poppe

Golsong

et al. 2011

European Journal of Cardio-Thoracic Surgery

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Section: Transfection Of Primary Skeletal Myoblastssupporting

confidence: 82%

Section: Assessment Of Hemodynamic Parametersmentioning

confidence: 84%

Section: Transfection Of Primary Skeletal Myoblastsmentioning

confidence: 95%

Section: Transfection Of Primary Skeletal Myoblastsmentioning

confidence: 99%

See 2 more Smart Citations

Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application

Blumenthal

Poppe

Golsong

et al. 2011

European Journal of Cardio-Thoracic Surgery

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show abstract

“…8 In brief, the plasmid pcDNA3-huda-Akt (expressing a myristinylated dominant-active form of Akt1) was delivered into SkM using the liposome-based transfection reagent Metafectene Pro (Biontex, Munich, Germany). According to the manufacturer's protocol, we incubated 5 g plasmid DNA (diluted in 100 l MEM) with 12 L Metafectene Pro solution (diluted in 100 l MEM) for 25 min at room temperature.…”

Section: Transfection Of Primary Skeletal Myoblastsmentioning

confidence: 99%

Scaffold-Based Transplantation of Vascular Endothelial Growth Factor—Overexpressing Stem Cells Leads to Neovascularization in Ischemic Myocardium but Did Not Show a Functional Regenerative Effect

et al. 2012

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The transplantation of skeletal myoblasts (SkM) might improve cardiac function after myocardial infarction via paracrine action. We used scaffold-based cell transfer by using vascular endothelial growth factor (VEGF)-overexpressing myoblasts. Skeletal myoblasts were isolated and expanded from newborn Lewis rats. Cells were transfected with pCINeo-VEGF(121) and seeded on polyurethane (PU) scaffolds. The seeded scaffolds were epicardially implanted in rats 2 weeks after myocardial infarction (group: PU-VEGF-SkM). Before this intervention and 6 weeks later, pressure/volume loops were analyzed followed by histology. Additional study groups (n = 10 per group) were injected with VEGF-overexpressing myoblasts (Inj-VEGF-SkM) or unmodified myoblasts (Inj-SkM) or underwent a sham operation. Overexpression of VEGF was verified in vitro. The transplantation of growth factor producing myoblast-seeded scaffolds resulted in enhanced angiogenesis of ischemically damaged myocardium in vivo. However, the infarction size was not reduced. In group Inj-SkM, hemodynamics remained unchanged. Systolic function as measured by dP/dt(max) was not significantly altered in PU-VEGF-SkM (preinterventionally 2,156 ± 1,222 mmHg vs. postinterventionally 2,134 ± 850 mmHg). Other systolic function and diastolic function parameters as measured by dP/dt(min), tau, and pressure half-time were not restored in groups PU-VEGF-SkM and Inj-VEGF-SkM either. Transplantation of VEGF-overexpressing skeletal myoblasts leads to neovascularization in infarcted hearts. No functional myocardial recovery was observed. Scaffold-based transfer of genetically-modified cells remains a useful tool to study paracrine stem cell action.

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Bioprinting of Cardiac Tissues

Cheung

Duan

Butcher

2015

Essentials of 3D Biofabrication and Translation

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Polyurethane scaffolds seeded with genetically engineered skeletal myoblasts: a promising tool to regenerate myocardial function

Cited by 4 publications

References 0 publications

Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application

Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application

Scaffold-Based Transplantation of Vascular Endothelial Growth Factor—Overexpressing Stem Cells Leads to Neovascularization in Ischemic Myocardium but Did Not Show a Functional Regenerative Effect

Bioprinting of Cardiac Tissues

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