Polytene chromosomes of the flesh-fly parasarcophaga argyrostoma (Sarcophagidae: Diptera)

Kaul, D.; Tewari, Raghav Ram

doi:10.1007/bf00120565

Cited by 6 publications

(7 citation statements)

References 12 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the sarcophagids thus far examined have 2nϭ12, with 5 pairs of meta/submetacentric autosomes and a pair of sex chromosomes (Stevens 1908, Metz 1916, 1922, Keneuke 1924, Boyes 1953, 1963, Boyes and van Brink 1965, Kaul et al 1978, Parise-Maltempi and Avancini 2000, present study). The only variations from this theme are Pseudosarcophaga affinis with 2nϭ19/20 (Boyes and van Brink 1965) and Helicophagella melanura 2nϭ14 (Tewari and Kurahashi 1985).…”

Section: Discussionmentioning

confidence: 69%

“…All the species studied so far are characterized by the presence of 5 polytene chromosomes, corresponding to the 5 pair of autosomes. The 5 polytene elements reveal almost identical banding patterns except for inversions in a few chromosomes (Whitten 1969, Trepte 1976, Kaul and Tewari 1978. The polytene chromosome comparisons in 6 species of the genus Boettcherisca also reveal similar banding patterns, except for paracentric inversion differences in chromosome II, III and VI (Tewari et al in preparation).…”

Section: Discussionmentioning

confidence: 93%

“…attributed to the accumulation of heterochromatin on both the X and Y chromosomes (Boyes and van Brink 1965, Kaul et al 1978, 1981, Tewari 1988. It is noteworthy that all the species of Boettcherisca possess dot-like sex chromosomes.…”

Section: Discussionmentioning

confidence: 99%

“…The ganglion cells were stained with 2% lacto aceto orcein for 30 min and conventional squash preparations were made in 45% acetic acid. The chromosomal analysis was carried out following the method of Kaul et al (1978). The nomenclature of Levan et al (1964) was used to describe the morphology of chromosomes.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Cytogenetics of Flesh Flies of the Genus Boettcherisca (Sarcophagidae: Diptera)

Agrawal¹,

Bajpai

Tewari

et al. 2010

CYTOLOGIA

View full text Add to dashboard Cite

SummaryThe present study comprises a description of mitotic chromosomes and the distribution of AT-rich DNA in the complements of 9 species of flesh flies of the genus Boettcherisca, B. koimani, B. karnyi, B. nepalensis, B. invaria, B. septentrionalis, B. peregrina, B. timorensis, B. nathani and B. javanica. All the species have 12 chromosomes in their diploid complement with 5 pairs of meta/submetacentric autosomes and a pair of small dot-like sex chromosomes-XX in the females and XY in the males. Staining with fluorochrome DAPI/AMD reveals distinct fluorochrome bright areas on centromeric regions of some chromosomes of each species. However, the distributions of AT-rich heterochromatic areas do not follow any regular pattern among the 9 species.Key words Boettcherisca, Mitotic chromosomes, Heterochromatin, DAPI, Fluorescence.Flesh flies of the genus Boettcherisca are distributed in the tropical and temperate areas of Indo-Australian region. The genus includes 15 species, which have the typical appearance of flesh flies i.e. 3 black longitudinal stripes on the scutum, and black-and-white checkered pattern on the abdomen. The adults of these species can only be distinguished on the basis of their male genitalia (Kano and Shinonaga 1977, Kano and Sugiyama 1983, Pape 1996. Phenetic and phyletic analysis of 11 species from the genus, based on numerical taxonomic methods, has revealed the existence of 2 monophyletic subgroups, namely the peregrina subgroup including peregrina-nathani-javanicatimorensis and the septentrionalis subgroup consisting of septentrionalis-formosensis-cabreraiinvaria, as well as the paraphyletic karnyi subgroup of hypothetical ancestor-karnyi-nepalensiskoimani (Kurahashi and Kano 1984).Present paper describes mitotic chromosomes and distributions of AT rich heterochromatic areas in the mitotic complement of 9 species belonging to the genus Boettcherisca and its bearing on relationship of the 3 subgroups, if any. Material and methodsNine species of flesh flies belonging to the genus Boettcherisca Rohdendorf were collected from different places (Table 1). Stock cultures of all the species were started from single wild female collected on bait in the field and routinely maintained in the laboratory (Kurahashi and Ohtaki 1989).Neural ganglia from colchicine pre-treated third instar larvae were dissected out in saline (0.75%), incubated in hypotonic solution (0.45% sodium citrate in double distilled water) for 5 min, then fixed in freshly prepared aceto alcohol (1 part glacial acetic acid and 3 part ethanol). The

show abstract

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Cytogenetics of Flesh Flies of the Genus Boettcherisca (Sarcophagidae: Diptera)

Agrawal¹,

Bajpai

Tewari

et al. 2010

CYTOLOGIA

View full text Add to dashboard Cite

show abstract

“…The diploid number of chromosomes in most sarcophagid species, was previously noted as 2n=12 [11,12,13,14,15,16,17,18,19,20] . However, 2n= 19-20 and 2n=14 have been recorded for Pseudosarcophaga affinis [14] , and Helicophagella melanura [21] , respectively.…”

Section: Main Bodymentioning

confidence: 98%

The karyotype of the forensically important flesh flies (Diptera: Sarcophagidae), Mini Review

El-Bassiony¹

2022

Preprint

View full text Add to dashboard Cite

Maggots of flesh flies were known as one of the main players in the depletion of earthily decomposed remains. They colonized the carrion few minutes after death, and were known as the initial colonizers in several research papers. In consequence, they could be used in the minimum post-mortem intervals (PMImini) estimation. Also, some sarcophagid species proved capable of detecting several toxins/ drugs in carcasses in the entomotoxicological research. The difficulty to identify sarcophagid females and larvae hindered their role as entomological evidence. Cytogenetics would aid in the identification of the immatures and their sex; the differentiation between adult species; and/ or the categorization of the taxa. Sarcophagidae received little attention concerning their karyotype measurements, especially in Egypt where there is only one unpublished thesis (El-Bassiony 2001). This mini review listed the previous studies and findings on sarcophagid’s karyotypes, especially the solitary data from Egypt.

show abstract

Interspecific comparisons of polytene chromosomes in the genus Parasarcophaga (Sarcophagidae: Diptera)

Kaul

Tewari

1983

Genetica

Self Cite

View full text Add to dashboard Cite

A comparison of the polytene chromosomes of Parasarcophaga argyrostoma, P. rufi'cornis, P. misera and P. knabi, is presented. There is close resemblance in the banding patterns of the four species. Two independent paracentric inversions in chromosome arm I1R, a small paracentric inversion in chromosome arm II1R and a pericentric inversion in chromosome V were found to be fixed as interspecific differences.

show abstract

Polytene chromosomes of the flesh-fly parasarcophaga argyrostoma (Sarcophagidae: Diptera)

Cited by 6 publications

References 12 publications

Cytogenetics of Flesh Flies of the Genus Boettcherisca (Sarcophagidae: Diptera)

Cytogenetics of Flesh Flies of the Genus Boettcherisca (Sarcophagidae: Diptera)

The karyotype of the forensically important flesh flies (Diptera: Sarcophagidae), Mini Review

Interspecific comparisons of polytene chromosomes in the genus Parasarcophaga (Sarcophagidae: Diptera)

Contact Info

Product

Resources

About