The black soldier fly larvae (BSFL) may serve as a promising tool in the animals feed production industry. The input organic wastes may be contaminated by insecticides that affect both the insect’s mass rearing, and the animals feed process. Therefore, in the current study the assessment of oxidative stress parameters of the black soldier fly (BSF) were investigated to quantify the deleterious effect of malathion-contaminated kitchen waste (1:1 vegetable: fruit waste) container on the insect. The different developmental stages of insect (adult and larva) were exposed to different concentrations (0, 0.005, 0.01, 0.015, and 0.02 mg/mL) of malathion. The results showed that the mean value of the reactive oxygen species (ROS), which included hydrogen peroxide (H2O2) and superoxide anion radicals (O2•-) concentrations were lower in larval stage than in adults, in all treated groups (0, 0.005, 0.01, 0.015, and 0.02 mg/mL malathion concentration). Also, the protein carbonyls amount and lipid peroxides levels were decreased in the 0.02 mg/mL Malathion compared to the control values. However, the cluster analysis revealed slight dissimilar patterns for control insects and the highest malathion concentration (0.02 mg/ml). These stage-related differences could occur from the different growth dynamic functions of larvae and adults. The larvae were distinguished by robust growth, and significant oxygen consumption. The results verified that oxidative stress parameters, especially protein carbonyls and α, α-diphenyl-β-picrylhydrazyl (DPPH) were promising, cheap, quick and cost-effective applications for determining the macromolecules damage, and antioxidant ability of H. illucens enclosed with malathion exposure. These findings described that malathion application induces macromolecules damage mediated through oxidative stress injury.

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The present study was designed to test the hypothesis that house flies may be capable of specifically harbouring ingested Vibrio cholerae in their digestive tracts. Flies were continuously fed green fluorescent protein (GFP)-labelled, non-O1/non-O139 environmental strains of V. cholerae. Bacterial burdens were quantitatively measured using plate counts and localization was directly observed using confocal microscopy. Vibrio cholerae were present in the fly alimentary canal after just 4 h, and reached a plateau of ∼10 colony-forming units (CFU)/fly after 5 days in those flies most tolerant of the pathogen. However, individual flies were resistant to the pathogen: one or more flies were found to carry < 180 V. cholerae CFU at each time-point examined. In flies carrying V. cholerae, the pathogen was predominantly localized to the midgut rather than the rectal space or crop. The proportion of house flies carrying V. cholerae in the midgut was dose-dependent: the continuous ingestion of a concentrated, freshly prepared dose of V. cholerae increased the likelihood that fluorescent cells would be observed. However, V. cholerae may be a transient inhabitant of the house fly. This work represents the first demonstration that V. cholerae can inhabit the house fly midgut, and provides a platform for future studies of host, pathogen and environmental mediators of the successful colonization of this disease vector.

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Defense peptides and proteins constitute key factors in insect humoral immune response against invading microorganisms. In this study, biochemical approach was designed to purify and characterize two peptides which appeared in larval haemolymph of B. mori after bacterial challenge. The results showed a significant increase of total protein of the bacterial-challenged haemolymph and then declined over time. This suggested that the AMPs are upregulated and released in haemolymph as "acute phase response" of the insect. Full antimicrobial activity was observed for the immune haemolymph at 24 h p.i. Fractionation of the immune haemolymph extract on a reversed phase C-18 column allowed effective separation of 5 fractions containing mainly proteins and peptides of molecular masses below 20 kDa. After fractionation, one out of three fractions (fraction# 5) exhibited the strongest antimicrobial activity. Finally, two peptides (5.8 and 4.3 KDa) were purified and one of them (4.3 KDa) showed full antimicrobial activity and very weak hemolytic activity up to concentration of 100 µg/ ml. These results were consistent to the results of quantitative protein analysis. Conclusively, this study demonstrated that the antimicrobial activity of the immune haemplymph is related to the presence of two antimicrobial peptides.