Polyphenols content of spent coffee grounds subjected to physico-chemical pretreatments influences lignocellulolytic enzymes production by Bacillus sp. R2

Khelil, Omar; Choubane, Slimane; Cheba, Ben Amar

doi:10.1016/j.biortech.2016.03.112

Cited by 26 publications

(11 citation statements)

References 19 publications

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“…275, Bacillus sp. R2, B. velezensis 157 and B. velezensis ZY-1-1 [10,11,21,22] showed similar results as those achieved in our study. This indicated that the strain played a potential role in cellulose degradation.…”

Section: Identification and Cellulose-degrading Potential Of Bacillussupporting

confidence: 90%

“…Microbial degradation of lignocellulose is a green biological refining method with advantages over physical and chemical methods [9]. The bacterial genus Bacillus is an excellent degrader that exhibits various abilities for degrading lignocellulose biomass, including cellulose, hemicellulose and lignin [10,11]. Furthermore, genome sequencing, considered an efficient method for investigation of function, has been utilized in lignocellulose degradation research.…”

Section: Introductionmentioning

confidence: 99%

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Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

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Section: Identification and Cellulose-degrading Potential Of Bacillussupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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“…Bacillus sp. strains metabolize fiber and release phenolic compounds due to a lignocellulytic activity with a strong correlation between phenolic compounds and production of cellulases and pectinases (Khelil et al 2016). These enzymes have the ability to break down cell wall components of plant cells, thus achieving ester bond hydrolysis that bind the phenolic compounds to the cell wall after lignin degradation (Hur et al 2014).…”

Section: Effect Of Fermentation On Tpc Tf and Aamentioning

confidence: 99%

Increase of content and bioactivity of total phenolic compounds from spent coffee grounds through solid state fermentation by Bacillus clausii

et al. 2018

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Spent coffee grounds are waste material generated during coffee beverage preparation. This by-product disposal causes a negative environmental impact, in addition to the loss of a rich source of nutrients and bioactive compounds. A rotating central composition design was used to determine the optimal conditions for the bioactivity of phenolic compounds obtained after the solid state fermentation of spent coffee grounds by . To achieve this, temperature and fermentation time were varied according to the experimental design and the total phenolic and flavonoid content, antioxidant activity and antimicrobial activity were determined. Surface response methodology showed that optimum bioprocessing conditions were a temperature of 37 °C and a fermentation time of 39 h. Under these conditions, total phenolic and flavonoid contents increased by 36 and 13%, respectively, in fermented extracts as compared to non-fermented. In addition, the antioxidant activity was increased by 15% and higher antimicrobial activity was observed against Gram positive and negative bacteria. These data demonstrated that bioprocessing optimization of spent coffee grounds using the surface response methodology was an important tool to improve phenolic extraction, which could be used as an antioxidant and antimicrobial agents incorporated into different types of food products.

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“…Compared with physical and chemical methods, microbial lignocellulosic degradation is a green process [8]. Bacillus is generally an excellent degrader, with specific strains exhibiting various abilities for lignocellulose degradation, including the degradation of cellulose, hemicellulose, and lignin [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

Tang

Zheng

et al. 2019

Preprint

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Background: Compared to physical and chemical methods, microbial lignocellulosic degradation is a green process. Bacillus is an excellent organic matter degrader, and it has exhibited various abilities required for lignocellulose degradation, including the degradation of cellulose, hemicellulose and lignin. Several B. velezensis strains encode lignocellulosases. However, their usefulness based on their ability to transform biomass has not been appreciated. Genomic, comprehensive comparative genomic and secretomic analyses were used to clarify the lignocellulose-degrading potential of these bacteria, which is necessary for their future application.Results: In the present study, the complete genomes of 20 B. velezensis strains and an endophytic bacterium, B. velezensis LC1, were analysed to find common and unique genes encoding carbohydrate-active enzymes (CAZymes) and evaluate their potentials to degrade lignocellulose. By comparative whole genomic and CAZyomic analyses of all 21 strains, we identified genes of lignocellulolytic enzymes with the potential to degrade cellulose (GH5, GH30, GH4, GH1, GH16 and GH32) and hemicellulose (GH43, GH30, GH51, GH26 and GH53). Further identification of the secretome of B. velezensis LC1 by liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed that a considerable number of proteins in the culture medium are involved in lignocellulose degradation, including endoglucanase, hemicellulases, and other related proteins. Moreover, assays of the activities of several lignocellulolytic enzymes including endoglucanase, exoglucanase, β-glucosidase, xylanase, lignin peroxidase, laccase, and manganese peroxidase show that these enzymes are more active in bamboo powder compared to glucose substrates. After a 6-day treatment, the degradation efficiencies of cellulose, hemicellulose and lignin from bamboo powder were 59.90%, 75.44% and 23.41%, respectively. The hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 10.44 g/L ethanol after 96 h.Conclusions: These findings indicate that Bacillus velezensis strains have the potential to degrade a range of polysaccharides in lignocellulosic biomass. One strain, B. velezensis LC1, efficiently degrades bamboo lignocellulose components, allowing for subsequent ethanol production.

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Polyphenols content of spent coffee grounds subjected to physico-chemical pretreatments influences lignocellulolytic enzymes production by Bacillus sp. R2

Cited by 26 publications

References 19 publications

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Increase of content and bioactivity of total phenolic compounds from spent coffee grounds through solid state fermentation by Bacillus clausii

Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

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