Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Abstract

Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

“…However, lignocellulose hydrolysis and utilization remain considerable challenges in lignocellulose-derived biofuel and biochemical production [ 8 ]. At present, the main obstacles include lignocellulose pretreatment and hydrolysis, as well as the efficient fermentation of pentose derived from lignocellulosic hydrolysates into LA.…”

“…At present, the main obstacles include lignocellulose pretreatment and hydrolysis, as well as the efficient fermentation of pentose derived from lignocellulosic hydrolysates into LA. Many studies have evaluated lignocellulose degradation and biofuel and biochemical production by using physical [ 9 , 10 ], chemical, and biological [ 8 ] pre-treatments. Chemical methods are the most common pretreatment methods, including acid treatment, alkaline treatment, alkaline/oxidative treatment, wet oxidation, and ozonolysis [ 11 , 12 , 13 ].…”

Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

“…The assembled genome of B. velezensis LC1, contains 44 GHs, 38 GTs, 30 CEs, 3 PLs, 6 AAs, and 15 CBMs [23], was compared with 20 other B. velezensis strains [9][10][11][12][13][14][15][16][17][18][19][20][21][22] at whole genomic level. The results showed that B. velezensis LC1 was phylogenomically closely related to the B. velezensis DR-08 ( Fig.1), while B. velezensis LC1 strongly resemble B. velezensis S3-1 according to the CAZyome pro le (Fig.…”

“…However, their potential usefulness based on their ability to transform biomass has not been appreciated [17][18][19][20][21][22]. To prove their potential usefulness, the whole genome sequences of 21 B. velezensis strains, including B. velezensis LC1 [23], were retrieved from the NCBI website and then submitted to dbCAN database to search carbohydrate-active enzymes (CAZymes), consist of six categories, encoding genes [24]. Next, we analyzed the secretome of B. velezensis LC1, and tested the degradation e ciency and enzyme activity change of B. velezensis LC1 to the alkali pretreated bamboo powder.…”

A Genomic Perspective on the Potential of Wild-Type Rumen Bacterium Enterobacter sp. LU1 as an Industrial Platform for Bio-Based Succinate Production

“…Genome sequencing was recognized as an efficient approach for investigation of gene functions [16,17]. Due to the potential use of Enterobacter sp.…”