High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Zhang, Zhenting; Li, Yanan; Jian-guo, Zhang; Peng, Nan; Liang, Yunxiang; Zhao, Siming

doi:10.3390/microorganisms8101491

Cited by 17 publications

(17 citation statements)

References 32 publications

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“…The untreated corn stover (% w/w, on a dry basis) contained mainly cellulose (31.18 ± 0.23), hemicellulose (26.95 ± 0.14), and lignin (16.43 ± 0.27). The CS used in this study contained a lower amount of cellulose, while amounts of hemicellulose and lignin were comparable to those of reported works [5,10]. It was likely that differences in the components of CS might be due to its trait variations and growing lands.…”

Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Sosupporting

confidence: 73%

“…The inhibitors derived from the pretreatment of CS have adverse impact on sacchari cation by cellulase. The effect could be eliminated via the operation of washing, biodetoxi cation, sodium bisul te addition, etc [7,10,17]. However, washing would generate a large quantity of waste water while biodetoxi cation need a long period.…”

Section: Optimization Of Sscf Processmentioning

confidence: 99%

“…Many studies have evaluated lignocellulosic biomass degradation and LA production by using physical, chemical and biological pretreatments. Among these methods of pretreatments, dilute acid pretreatment was used to e ciently produce lactic acid [7,9,10]. A high LA titer of 101.9 g/L was obtained from sulfuric acid-pretreated and biodetoxi ed corn stover (CS).…”

Section: Introductionmentioning

confidence: 99%

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The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Sun¹,

Li²,

Wu³

et al. 2021

Preprint

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Background: Lignocellulosic feedstocks have attracted much attention as an alternative carbon source for lactic acid (LA) production with the advantages of ready availability, sustainability, and renewability. However, the production of LA from lignocellulose faces at least two major technical obstacles. The inhibitors derived from pretreatment of lignocellulose inhibit the growth of microorganism used in downstream hydrolysis and fermentation processes. In addition, most LA producers cannot to ferment pentose sugars and have carbon catabolite repression (CCR) effect. Microbial consortium with great robustness can use complex feedstocks displaying high bioconversion efficiency and has received great attention nowadays.Results: in this study, a thermophilic LA producing consortium DUT50 was enriched and employed to enhance the utilization of corn stover (CS). Enterococcus was the dominant family, accounting for 93.66% abundance in DUT50, and the abundance of Lactobacillus, Bacillus, Lactococcus and Trichococcus, accounted for 2.68% in total. This consortium was highly resistant to inhibitors up to 10.90 g/L derived from pretreatment of CS, metabolized hexose and pentose simultaneously without CCR effect. Based on consortium promising features, an efficient process of simultaneous saccharification and co-fermentation (SSCF) was developed to produce LA from acid-pretreated corn stover. The economical route avoided the operations of solid–liquid separation and detoxification. The key influential factors, including dry biomass and cellulase loading, corn steep liquor powder concentration, and the pre-hydrolysis time were investigated and optimized. The highest LA titer of 71.04 g/L with a yield of 0.49 g/g-CS was achieved at a dry biomass loading of 20% (w/v). This is the reported highest LA production from non-detoxified acid-pretreated corn stover via the SSCF process without wastewater generation. The interaction mode of Enterococcus was collaboration while the low abundance of Lactobacillus and Bacillus might metabolize xylose efficiently via the pentose phosphate pathway.Conclusions: Our results demonstrated the potential advantage of symbiosis and provided a feasible and economical route to produce LA from lignocellulosic biomass in industrial scale.

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Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Sosupporting

confidence: 73%

Section: Optimization Of Sscf Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Sun¹,

Li²,

Wu³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The values of OD 600 can represent the dynamic growth rates of the microbial cells [ 31 , 32 ]. From Figure 1 a, it was found that the values of OD 600 at day 1 during fermentation with various media were appropriately 0.14 ± 0.01.…”

Section: Resultsmentioning

confidence: 99%

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

et al. 2021

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The high operating cost is currently a limitation to industrialize microbial lipids production by the yeast Lipomyces starkeyi. To explore economic fermentation technology, the two-stage fermentation of Lipomyces starkeyi using yeast extract peptone dextrose (YPD) medium, orange peel (OP) hydrolysate medium, and their mixed medium were investigated for seven days by monitoring OD600 values, pH values, cell growth status, C/N ratios, total carbon concentration, total nitrogen concentration, residual sugar concentration, lipid content, lipid titer, and fatty acids profiles of lipids. The results showed that two-stage fermentation with YPD and 50% YPD + 50% OP medium contributed to lipid accumulation, leading to larger internal lipid droplets in the yeast cells. However, the cells in pure OP hydrolysate grew abnormally, showing skinny and angular shapes. Compared to the one-stage fermentation, the two-stage fermentation enhanced lipid contents by 18.5%, 27.1%, and 21.4% in the flasks with YPD medium, OP medium, and 50%YPD + 50%OP medium, and enhanced the lipid titer by 77.8%, 13.6%, and 63.0%, respectively. The microbial lipids obtained from both one-stage and two-stage fermentation showed no significant difference in fatty acid compositions, which were mainly dominated by palmitic acid (33.36–38.43%) and oleic acid (46.6–48.12%). Hence, a mixture of commercial medium and lignocellulosic biomass hydrolysate could be a promising option to balance the operating cost and lipid production.

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“…Many studies have been conducted to evaluate the degradation of lignocellulosic biomass and the production of LA using physical, chemical, and biological pretreatments. Dilute acid pretreatment was used among these pretreatment methods to efficiently produce lactic acid [ 7 , 9 , 10 ]. A high LA titer of 101.9 g/L was obtained from sulfuric acid pretreated and bio-detoxified corn stover (CS).…”

Section: Introductionmentioning

confidence: 99%

The advanced performance of microbial consortium for simultaneous utilization of glucose and xylose to produce lactic acid directly from dilute sulfuric acid pretreated corn stover

Sun

Wu³

et al. 2021

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Lignocellulosic feedstocks have attracted much attention as a potential carbon source for lactic acid (LA) production because of their ready availability, sustainability, and renewability. However, there are at least two major technical challenges to producing LA from lignocellulose. Inhibitors derived from lignocellulose pretreatment have a negative impact on the growth of cells producing LA. Furthermore, pentose sugars produced from the pretreatment are difficultly utilized by most LA producers, which is known as the carbon catabolite repression (CCR) effect. This complex feedstock can be utilized by a robust microbial consortium with high bioconversion efficiency. Results In this study, a thermophilic consortium DUT50 producing LA was enriched and employed to improve corn stover (CS) utilization. Enterococcus was the dominant family in the consortium DUT50, accounting for 93.66% of the total abundance, with Lactobacillus, Bacillus, Lactococcus, and Trichococcus accounted for the remaining 2.68%. This consortium could be resistant to inhibitors concentration up to 9.74 g/L (2.88 g/L acetic acid, 2.46 g/L furfural, 2.20 g/L 5-HMF, and 2.20 g/L vanillin derived from pretreatment of CS), and simultaneously metabolizes hexose and pentose without CCR effect. Based on the promising consortium features, an efficient process of simultaneous saccharification and co-fermentation (SSCF) was developed to produce LA from acid pretreated corn stover, in which solid–liquid separation and detoxification were avoided. The key influencing factors were investigated and optimized, including dry biomass and cellulase loading, corn steep liquor powder concentration, and the pre-hydrolysis time. The highest LA titer of 71.04 g/L with a yield of 0.49 g/g-CS was achieved at a dry biomass loading of 20% (w/v), which is the highest LA production from non-detoxified acid pretreated corn stover via the SSCF process without wastewater generation reported to date. The simultaneous metabolism of hexose and pentose revealed collaboration between Enterococcus in the consortium, whereas xylose may be efficiently metabolized by Lactobacillus and Bacillus with low abundance via the pentose phosphate pathway. Conclusions The experimental results demonstrated the potential advantage of symbiosis in microbial consortia used for LA production from lignocellulosic biomass.

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High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Cited by 17 publications

References 32 publications

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

The advanced performance of microbial consortium for simultaneous utilization of glucose and xylose to produce lactic acid directly from dilute sulfuric acid pretreated corn stover

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