Polyphenolic content and bioactivities of Crataegus oxyacantha L. (Rosaceae)

Abstract

Solid-Liquid Extraction (SLE) using solvent of different polarities (CHCl 3 , EtOAc, and n-BuOH) has been applied to leaves and fruits from (Crataegus oxyacantha L.), a deciduous shrub with an expected rich phytochemical profile. The total polyphenols content and the radical scavenging activity of each extract were evaluated. These extracts were analyzed by HPLC-DAD and rutin, quercetin-3-glucoside, caftaric and caffeic acid had been positively identified. The phytochemical study of the ethyl acetate extract of C. oxyacantha , led to the isolation and structural elucidation of quercetin (1); quercetin-3-O-β-glucoside (2); epicatechin (3); naringenin (4), reported for the first time from this species except caffeic acid and epicatechin. These compounds were identified by 1D and 2D NMR combined analysis as well as by MS and UV.The antimicrobial activity of these extracts has also been tested, showing strong antibacterial activity-solvent dependent-against Gram positive bacteria. Additionally, bactericidal power was demonstrated in fruit extracts.

Exploring the powerful phytoarsenal of white grape marc against bacteria and parasites causing significant diseases

“…Their employment as additives is broadly distributed in industry field to delay, prevent or eliminate damage caused by oxidation. Recently, some plant extracts have been reported to have good antioxidant and antimicrobial properties due to their polyphenolic and protein compounds (Piscopo et al 2019;Tortora et al 2019;Benabderrahmane et al 2018;Benabderrahmane et al 2019). Due to its complex composition, containing antioxidant dietary fibre, as well as extractable and non-extractable polyphenol content, grape marc extracts have a great potential to display extensive uses in agricultural, pharmaceutical and cosmetic industries among others (Bargiacchi et al 2017;Placha et al 2013;de O Ribeiro et al 2018;Tayengwa and Mapiye 2018).…”

Botanical, Phytochemical, Anti-Microbial and Pharmaceutical Characteristics of Hawthorn (Crataegus monogyna Jacq.), Rosaceae

“…Hawthorn is a semi-evergreen shrub or small tree, that usually has thorns, mostly growing up to 5–15 m [ 12 , 13 ]. Leaf morphology, seed traits, the number of seeds and the color of the fruits are highly diverse.…”

“…Good yields of polyphenols extracted from hawthorn could be obtained [ 13 ]. Specifically, various chromatographic profiles revealed that both the leaves and berries of C. monogyna are rich in isoquercitrin, vitexin, quercetin, rutin and vitexin-2- rhamnoside.…”

“…The high-performance liquid chromatography with diode-array detection (DAD-HPLC) was analyzed to identify rutin, quercetin-3-glucoside, caftaric and caffeic acid. A structural elucidation of quercetin, quercetin-3- O -β-glucoside, epicatechin, naringenin was conducted [ 13 ]. A combination of 1D, 2D NMR, MS and UV was used.…”

Hawthorn (Crataegus spp.): An Updated Overview on Its Beneficial Properties

“…The work of Nabavi et al focused on the polyphenolic composition of C. monogyna Polyphenol compounds from C. oxyacantha extracts, including epicatechin, epicatechin gallate (ECG), rutin, caffeic, and caftaric acids, were identified using HPLC-DAD and LC-MS/MS techniques [27]. In a study, UV/MS analysis coupled with 1D/2D nuclear magnetic resonance (NMR) spectroscopy was used to detect the compounds extracted from the ethyl acetate extract of C. oxyacantha, which included naringenin, epicatechin, quercetin-3-O-β-glucoside, and quercetin [28]. The presence of rutin and quercetin obtained from C. oxyacantha extracts using HPLC was also reported [29].…”