Polymorphonuclear leukocyte collagenase: Localization in granules subjected to zonal centrifugation

Abstract

Abstract. Polymorphonuclear leukocytes were obtained from rabbit peritoneal exudates. Granule preparations from these cells were subjected to zonal fractionation through a discontinuous sucrose gradient. Azurophilic and specific granules were enzymatically characterized by peroxidase and alkaline phosphatase, respectively. Collagenase was consistently associated with the alkaline phosphatase‐containing specific granules.