Hansen, T. M. Collagen development in granulation tissue as compared with collagen of skin and aorta from injured and non-injured rats. Acta path. microhiol. scand. Sect. A, 83: 721-732, 1975. Granulation tissue in rats was produced by subcutaneous implantation of viscose cellulose sponges.Granulomas, aortae, and skin samples were taken 4, 8, 14, 22, 33, and 42 days after the sponge implantation and compared with age-matched non-operated rats. 1 G p r o l i n e was given 4 hours before death to animals killed on day 0, 14, and 42. The "C-OH-proline activity in salt insoluble collagen was higher in granulation tissue and aorta than in skin. This indicates a faster formation, or an increased stability of the intermolecular cross-links in granulation tissue and aorta, than in skin. The percentage of free OH-proline was higher in granulation tissue than in skin, reflecting a relatively increased collagen degradation in granulation tissue. An increased collage:^ degradation may also, in part, explain a registered higher alpha/beta ratio in collagen from granulation tissue than from skin, as well as the increase in alphdbeta ratio in the older granulomas. The sponge implantation did not affect the collagen of aorta and skin, but caused a decrease in the dry weight of aorta and skin, and an increase in the number of granulocytes in the blood.