Polymorphisms in 5′-flanking regions of genes encoding adiponectin, leptin, and resistin are not associated with obesity of Polish children and adolescents

Cieślak, J.; Skorczyk, Anna; Stachowiak, Monika; Szydłowski, M.; Grzes, Maria; Paczyńska, Paulina; Skowrońska, Bogda; Majewska, Katarzyna Anna; Stankiewicz, Witold; Fichna, Piotr; Świtoński, M.

doi:10.1007/s11033-010-0294-9

Cited by 15 publications

(14 citation statements)

References 24 publications

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“…However, there have been reports of differences in the frequencies of the 420C/G in other ethnic groups (Mattevi et al, 2004;Onuma et al, 2010;Chen et al, 2010;Cieslak et al, 2011). For the 44G/A and 62G/A polymorphisms, the frequencies of the AA genotype were < 3% in our study; these results were also reported in other populations (Cao and Hegele, 2001;Sentinelli et al, 2002;Conneely et al, 2004;Gouni-Berthold et al, 2005;Duman et al, 2007).…”

Section: Discussionsupporting

confidence: 61%

Association Between FourResistinPolymorphisms, Obesity, and Metabolic Syndrome Parameters in Tunisian Volunteers

Boumaiza

Omezzine

Rejeb

et al. 2012

Genetic Testing and Molecular Biomarkers

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Resistin is an adipocyte-secreted cytokine recently discovered and has been proposed as a link between obesity and diabetes. Many resistin gene polymorphisms were described and their implication in obesity and metabolic syndrome (MetS) was controversial. Our aim was to study the relationship between four resistin polymorphisms (420C/G, 44G/A, 62G/A, and 394C/G), MetS parameters, and the risk of obesity in Tunisian volunteers. We recruited 169 nonobese (sex ratio=0.594; mean age=43.25±13.12 years; mean body mass index [BMI]=24.73±3.50 kg/m(2)) and 160 obese subjects (sex ratio=0.221; mean age=48.41±10.92 years; mean BMI=36.6±4.8 kg/m(2)). Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. Anthropometric parameters, lipid levels, glycemia, and insulinemia were measured. BMI was calculated and insulin resistance was evaluated with the homeostasis model assessment insulin resistance (HOMA-IR). Statistical analyses were performed by SPSS 17.0. The 420C/G seems to contribute to obesity. In fact adjusted odds ratio (OR) of obesity associated to mutated genotypes was 2.17 and 95% confidence interval was 1.28-3.68 (p=0.004). Mutated genotypes at 420C/G were associated with higher waist circumference and BMI. In addition, 44G/A polymorphism was associated with increased total cholesterol and low-density lipoprotein-cholesterol levels. The other genotypes showed no association with MetS parameters. Concerning association between single-nucleotide polymorphisms and MetS risk, only mutated genotypes at 44G/A increase the risk of MetS after adjustment to confounding parameters (OR=1.93, p=0.023). In conclusion, resistin gene polymorphisms 420C/G and 44G/A were associated with obesity and MetS parameters in Tunisian volunteers.

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Section: Discussionsupporting

confidence: 61%

Association Between FourResistinPolymorphisms, Obesity, and Metabolic Syndrome Parameters in Tunisian Volunteers

Boumaiza

Omezzine

Rejeb

et al. 2012

Genetic Testing and Molecular Biomarkers

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“…Although no studies have analyzed the association of the rs10401670 SNP with anthropometric variables, contradictory results can also be found in previous studies of other polymorphisms in the resitin gene in adults [24-26, 28, 33, 34]. The study by Cieslak et al [29], which examines the relationship of the several polymorphisms in the resistin gene with obesity in Polish children, failed to find any significant association. Unfortunately, we do not have data on body fat composition from a sufficiently large sample so as to analyze the association of these SNPs with body fat mass.…”

Section: Discussionmentioning

confidence: 93%

“…However, studies in other populations have failed to find an association with anthropometric variables [22,24,28] or lipid profile [17,19,25]. The association of the SNP rs1862513 with obesity has also been the subject of studies in children [29], but to our knowledge its association with lipid levels has not been investigated in this age group.…”

Section: Introductionmentioning

confidence: 94%

Association of resistin polymorphisms with resistin levels and lipid profile in children

et al. 2014

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Previous research has found a correlation between resistin and lipid level variations. Polymorphisms in the resistin gene (RETN) could be involved in this relationship, but the results of the different studies are contradictory. The aim of this study was to examine the association between resistin and lipid levels, and to determine whether resistin polymorphisms are associated with resistin levels and lipid profile in prepubertal children and adolescents. The single nucleotide polymorphisms (SNPs) rs1862513 and rs10401670 were analyzed in 442 randomly selected 6- to 8-year-old children and 827 children aged 12-16 years. Anthropometric data were recorded. Lipid profile was determined using standard methods. Serum resistin levels were measured using a multiplexed bead immunoassay. Resistin polymorphisms were determined by TaqMan(®) allelic discrimination assays. A relationship was found between serum levels of resistin and the SNP rs10401670 in 6- to 8-year-old boys. SNP rs10401670 was also related to TC and LDL-cholesterol in 12- to 16-year-old boys and to HDL-C in 12- to 16-year-old girls. SNP rs1862513 was not related to any of the studied variables. Serum resistin levels were significantly and negatively associated with ApoAI levels in 12- to 16-year-old girls. A SNP in the 3'UTR region of RETN (rs10401670) is associated with resistin levels and lipid profile in children, showing different associations depending on age and gender.

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“…For all the 48 patients genotypes at five polymorphic sites (three in LEP and two in ADIPOQ ) in the 5′-flanking regions of both genes were established in our earlier study [14]. The comparison of gene expression, including all the three genotypes (two alternative homozygotes and a heterozygote) was possible only for the three most common SNPs, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…There are also reports indicating an association between obesity and some polymorphisms in the 5′-flanking regions of the leptin ( LEP ) and adiponectin ( ADIPOQ ) genes; however, the conclusions are not consistent [9–13]. In our recent studies of obese children and adolescents we detected 9 polymorphic sites in the 5′-flanking region of the LEP and 2 in the 5′-flanking region of the ADIPOQ genes [14], but the frequency of these variants did not differ in a cohort of obese patients when compared with non-obese ones. The effect of polymorphism in the 5′-flanking regions of genes encoding adiponectin and leptin was reported in several studies.…”

Section: Introductionmentioning

confidence: 89%

Effect of three common SNPs in 5′-flanking region of LEP and ADIPOQ genes on their expression in Polish obese children and adolescents

et al. 2011

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Genes encoding adipokines are considered as candidates for human obesity. In this study we analyzed the expression of leptin (LEP) and adiponectin (ADIPOQ) genes in relation to common 5′-flanking or 5′UTR variants: -2548G>A (LEP), 19A>G (LEP) and -11377C>G (ADIPOQ) in Polish obese children and adolescents. Relative transcription levels in the subcutaneous adipose tissue (real time RT–PCR) and serum protein concentrations (RIA) were measured in 48 obese subjects with known genotypes at three polymorphic sites and in five non-obese controls. None of the studied polymorphisms altered significantly the expression. Significantly elevated relative transcription levels of the LEP gene (P < 0.05) and serum leptin concentrations (P < 0.01) were recorded in obese patients, when compared with the non-obese controls, but such differences were not found for the ADIPOQ gene. Interestingly, the leptin to adiponectin protein concentration ratio (L/A) was approximately sevenfold higher in obese children and adolescents when compared with the non-obese controls (P < 0.001). Taking into consideration the observed relationship between the genotypes and the gene expression level we suggest that these SNPs are not conclusive markers for predisposition to obesity in Polish children and adolescents. On the other hand, we confirmed that the leptin to adiponectin gene expression ratio (L/A) is an informative index characterizing obesity.

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Polymorphisms in 5′-flanking regions of genes encoding adiponectin, leptin, and resistin are not associated with obesity of Polish children and adolescents

Cited by 15 publications

References 24 publications

Association Between FourResistinPolymorphisms, Obesity, and Metabolic Syndrome Parameters in Tunisian Volunteers

Association Between FourResistinPolymorphisms, Obesity, and Metabolic Syndrome Parameters in Tunisian Volunteers

Association of resistin polymorphisms with resistin levels and lipid profile in children

Effect of three common SNPs in 5′-flanking region of LEP and ADIPOQ genes on their expression in Polish obese children and adolescents

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