Polymorphism of the vitamin D3 receptor in patients with psoriasis

Abstract

1,25-Dihydroxyvitamin D is the biologically active form of vitamin D for the treatment of skin eruptions in patients with psoriasis. 1,25-(OH)(2)D(3) elicits its action on skin eruptions through the vitamin D receptor (VDR). Allelic frequencies of VDR were studied in 86 normal subjects and 50 patients with psoriasis. Genomic DNA was extracted from peripheral blood leukocytes and the VDR gene was amplified using a heminested polymerase chain reaction (PCR). The products were digested with respective restriction enzymes ApaI, TaqI and BsmI. The restriction fragment length polymorphisms (RFLP) were coded as Aa, Tt or Bb. The frequencies of ApaI, BsmI and TaqI RFLP genotypes in psoriasis patients showed no significant differences compared with normal controls. The frequency of the AA genotype was significantly higher in pustulosis palmaris et plantaris patients than in psoriasis vulgaris patients ( P<0.05), and in psoriasis vulgaris patients than in psoriasis pustulosa patients ( P<0.01). In patients with psoriasis, the levels of serum alanine 2-oxoglutarate aminotransferase (ALT) were significantly higher in patients with the AA genotype (54.0+/-22.0 IU/l, n=4) than in those with the aa genotype (24.0+/-15.9 IU/l, n=27; P<0.02). The distribution of ApaI, BsmI, TaqI RFLP VDR genotypes showed no significant relationship to the PASI score, serum aspartate 2-oxoglutarate aminotransferase or triglyceride levels, or age at onset. These results show that the VDR genotype contributes to the liver dysfunction in patients with psoriasis, although no correlation was found between VDR genotype and the skin eruptions of psoriasis.

UVB and Vitamin D in Psoriasis

“…Calcitriol is involved in the regulation of antimicrobial peptides cathelicidin and human -defensin 2 (HBD2), which both participate in the pathogenesis of psoriasis (22). Vitamin D's role in psoriasis is further supported by studies that confirm the link between VDR polymorphism and psoriasis (23,24). An association between VDR genotypes (Apa1) and the mean age at onset of psoriasis were previously observed (25).…”

Vitamin D receptor polymorphisms: no association with type 1 diabetes in the Finnish population

“…The gene encodes a polypeptide that binds 1,25-dihydroxy-calciferol (activated vitamin D) and interacts with target nuclei to produce a variety of effects. VDR has been shown to be present in significant concentrations in T lymphocytes and macrophages (19), and some of its polymorphisms have recently been found to be associated with several autoimmune diseases in man, such as psoriasis (20), Grave's disease (21,22), Addison's disease (23) and T1D (24 -31). In addition, the VDRF and VDRB single nucleotide polymorphisms (SNPs) were suggested to have an effect on GAD65-autoantibody positivity in T1D (30) and on the onset pattern of the disease (31), respectively, in Japanese case-control analyses.…”

Vitamin D receptor gene polymorphism is not associated with psoriasis in the Italian Caucasian population

“…In recent years, some studies [5][6][7][8][9][10] reported an association between psoriasis and VDR polymorphisms. In a Korean population, a significant difference in VDR genotypes between normal controls and psoriatic patients, especially those with early onset, was demonstrated [5].…”