Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds

Yılmaz, Onur; Sezenler, Tamer; Ata, Nezi̇h; Yaman, Yalçın; Cemal, İ̇brahim; Karaca, Orhan

doi:10.3906/vet-1401-13

Cited by 10 publications

(6 citation statements)

References 13 publications

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“…In general, the obtained values of genetic population analysis by calpastatin gene are in agreement with the characteristics for other foreign gene funds of sheep, for instance, sheep of Turkish selection (Avanus K. 2015, Yilmaz et al, 2014).…”

Section: Castsupporting

confidence: 84%

Untitled

2020

Agric. sci. pract.

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Aim. To determine the level of polymorphism of some genes, pertaining to the quantitative traits of sheep and to study the genetic diversity and population structure of breeds of Askanian sheep and one of its hybrids. Methods. Molecular-genetic, population-statistical, biometric methods. Results. The genetic structure of populations of the Askanian Fine-Fleeced (AFF, n = 33), Askanian Meat-and-Wool (AMW, n = 22) and Askanian Karakul (AK, n = 46) breeds and a hybrid of Askanian Fine-Fleeced × Texel (AFFT, n = 40) has been studied for the fi rst time in terms of the polymorphisms of the following qualitative trait genes (loci) : meat productivity genes: ovine growth hormone (GH), calpastatin (CAST) and myostatin (MSTN); prolifi cacy genes: Booroola (fecundity gene FecB) and bone morphogenetic protein (BMP15). The sheep in all studied breeds and the hybrid mentioned above are characterized by the polymorphism of two loci, GH and CAST. The other three loci studied did not show polymorphism for the markers studied. GH is present as two genotypes (A/A, A/B), and CAST as three genotypes (A/A, A/B, B/B). The remaining genes were found to be in the monomorphic state. The frequencies of certain genotypes and alleles change in the direction from fi ne-fl eeced to meat animals. For instance, the concentration of heterozygous growth hormone (GH) genotype A/B increases from 0.0 % (AFFB) to 38.2 % (AMWB), and the concentration of allele A -from 0.083 to 0.191. The live bodyweight of fi ne-fl eeced lambs with GH genotype A/B at birth equaled 4.5 kg, and those with homozygous genotype A/A -4.9 kg (P < 0.001). Therefore, homozygous genotype А/А determined the increased level of meat productivity of sheep. A similar dependence was established at birth for the Askanian Karakul breed. Conclusions. The specifi city of genetic structure of Askanian breeds and hybrid is polymorphism of the GH and CAST and absence of polymorphism of BMP15 and FecB genes, determining the prolifi cacy (reproduction) trait. According to the distribution of polymorphic loci variants, the gene pools of Askanian Meat-and-Wool breed and the local Askanian-Texel hybrid are closely related and therefore explains the same direction of their productivity. At the same time, all populations are in a state of genetic equilibrium according to Hardy-Weinberg, which indicates a high level of their consolidation. Sheep which have the A/A homozygote of the gene growth hormone are characterized by an increased level of the body weight development.

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Section: Castsupporting

confidence: 84%

Untitled

2020

Agric. sci. pract.

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“…In the current study genotype frequency of CAST-MspI-MM genotype was found highest in Akkaraman sheep breed. CAST-MspI-MM genotype frequencies was found also highest in other Turkish sheep breeds such as Akkaraman, Kıvırcık, Karacabey Merino, Imroz, İvesi and Çine Çaparı breeds, whereas, frequency of genotype MN was highest in the Sakız and Karya sheep breeds [36,37] . Similar to our findings CAST-MspI-MM frequency was also found highest in different sheep breeds among the world [19,20,[38][39][40] .…”

Section: Discussionmentioning

confidence: 83%

“…In the native Balkhi and Kajli sheep breeds in Pakistan, it was reported that individuals with the "MN" genotype were found to have more favourable daily live birth weights compared to those with other genotypes [22] . In the native Kıvırcık sheep breed in Turkey, individuals with the NN genotype were reported to have less favorable results in terms of daily live birth weight gains than those with different genotypes [36] . In the Russian breeds of the Soviet Merino and Salks, individuals with the MN genotype were reported to have more favorable results in terms of weaning weight and mean daily weight gain compared to BAYRAM, AKYÜZ, ARSLAN ÖZDEMİR, AKSEL, ÇINAR those with other genotypes [38] .…”

Section: Discussionmentioning

confidence: 99%

Akkaraman Kuzularında DGAT1, CAST ve IGF-I Gen Polimorfizmleri ve Sütten Kesim Yaşına Kadarki Canlı Ağırlık Artışı Üzerine Etkileri

Bayram¹,

Akyüz²,

Arslan³

et al. 2018

Kafkas Univ Vet Fak Derg

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Early live weight in sheep is important for lamb survival and average weight gain until slaughter. The aim of the present study was to investigate association between CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms and early live weights between birth and weaning age in Akkaraman lambs. A total of 374 lambs were genotyped for CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms by the polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The results of PCR-RFLP analysis showed that the SNPs had three genotypes of CAST-MspI polymorphism, two genotypes of DGAT1-AluI polymorphism and one genotype of IGF-1-Bsp143II polymorphism of these, CAST-MspI-MM, DGAT1-AluI-CC and IGF-1-Bsp143II-BB were the predominant genotypes in the Akkaraman sheep breed. The result of Chi-square analysis indicated that the Akkaraman sheep breed was in Hardy-Weinberg equilibrium for the investigated polymorphic genes. At the DGAT1 locus, the CT genotype showed significantly heavier birth weight (P=0.044) compared to CC genotype. CAST gene did not show any association for the investigated traits. The results of this study demonstrate that the CT genotype had a positive effect on birth weight in Akkaraman sheep. We concluded that further investigations are needed in DGAT1-AluI polymorphism and live weight at different ages in sheep.

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“…Table 1 shows the genotype and allele frequencies of CAST/MspI polymorphism in Kıvırcık crossbred ewes. (Yılmaz et al 2014), 0.92 in Shumen sheep (Georgieva et al 2015). However, M allele frequency was similar with 0.87 in Harri sheep (Saleha 2015), 0.88 in Balkhi and 086 Kajli sheep (Riaz et al 2012), 0.88 in Makui sheep (Tohidi 2013), 0.85 in Arabic Sheep (Mohammadi et al 2008).…”

Section: Resultsmentioning

confidence: 99%