Polymorphism of the KAP1.1, KAP1.3 and K33 genes in Merino sheep

Itenge-Mweza, T.O.; Forrest, Rachel; McKenzie, Grant; Hogan, Aileen; Abbott, Johanna; Amoafo, Omega Y.; Hickford, Jonathan G. H.

doi:10.1016/j.mcp.2007.04.002

Cited by 45 publications

(61 citation statements)

References 9 publications

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“…The length of the amplimers from KAP 1.1 alleles A, B and C were 341, 311, 281 bp respectively. The length YARDİBİ, GÜRSEL, ATEŞ, AKIŞ HACIHASANOĞLU, ÖZTABAK polymorphism of the KAP 1.1 gene determined in this investigation has previously been reported in Romney sheep and in Merino sheep [18,21] . Our results showed that A and B alleles frequencies were higher in Awassi than the others and also the C allele frequency was the highest in Chios breed.…”

Section: Discussionsupporting

confidence: 64%

“…The K33 locus were designed from a published sequence, Gen Bank nos.AY835598-AY835602 to amplify a 480 bp fragment of the K33; F:5´-CACAACTCTGGCTTGGTGAACTTG-3' , R:5´-CTTAGCCATATCTCGGATTCCCTC-3´ [18] . Amplification consisted of; initial denaturation 95°C for 1 min, followed by 30 cycles of 95°C for 30 s, annealing at 65°C for 30 s and extension at 72°C for 30 s with a final extension of 72°C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

“…The Polymerase Chain Reaction (PCR) volume of 25 µl contained; 1 U Taq DNA Polymerase (Fermentas Life Sci., Canada), 2-2.5 mL 10X PCR buffer, 1.5 mM MgCl 2 , 50-100 ng genomic DNA, 100mM dNTP (TaKaRa Biotechnology Co., Ltd., Japan), and 10 pmol of each primer [18] . PCR products was visualised after electrophoresis on a 2% agarose gel with a long-wavelength UV transilluminator (Thermo Fisher Scientific, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Sakız, Kıvırcık ve İvesi Koyun Irklarında KAP 1.1, KAP 1.3 ve K33 Gen Polimorfizmlerinin Belirlenmesi

Yardibi¹,

Gürsel²,

Ateş³

et al. 2015

Kafkas Univ Vet Fak Derg

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Keratin-associated proteins are major structural components of hair and wool fibres. They also play an important role in determining the properties of the fibre. This study is designed to examine variation in the genes encoding key keratin and keratin -associated proteins in Chios, Kivircik and Awassi sheep. Variation at these loci has the potential to be developed as genetic markers deal with wool traits. Blood samples were taken into 2 ml sterilized tubes with EDTA from vena jugularis for having genomic DNA samples. Genomic DNA isolation was obtained by standard salting out method. Regions were amplified for the determination of KAP 1.1, KAP 1.3 and K33 polymorphisms in DNA samples by using PCR. Gene polymorphisms and chi-square test were used to determine whether the populations are in Hardy-Weinberg equilibrium using by POPGENE32 software. Results of the sequence analysis of the regions were evaluated by using MEGA 5. The results have shown a possibility to improve the quality of the wool traits on those local species by doing selection trials targeting on those mentioned genes. Keywords

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Section: Discussionsupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Sakız, Kıvırcık ve İvesi Koyun Irklarında KAP 1.1, KAP 1.3 ve K33 Gen Polimorfizmlerinin Belirlenmesi

Yardibi¹,

Gürsel²,

Ateş³

et al. 2015

Kafkas Univ Vet Fak Derg

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“…Almost all of the fiber (90%) is composed of the cortex, which consists of keratin intermediate filaments embedded in a matrix of keratin-associated proteins (KAPs) (Kuczek and Rogers, 1987;Itenge-Mweza et al, 2007). KAPs cross-link the keratin intermediate filaments with inter-chain disulfide-bonding, and form the wool fiber after keratinization (Gong et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

et al. 2014

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ABSTRACT. High glycine-tyrosine proteins (HGTPs), also known as keratin-associated proteins (KAPs), play a key role in the major structures and mechanical properties of wool fiber. Sheep HGTPs consist of three multigene families: KAP6, KAP7, and KAP8 genes. Polymorphisms of these three genes have been proposed to have important effects on wool fiber traits. The aim of the present study was to identify polymorphisms of the KAP6, KAP7, and KAP8 genes in four sheep breeds, including Chinese Merino superfine wool sheep, Hu sheep, a Merino x Hu crossed breed, and Romney sheep. Polymerase chain reaction (PCR) product direct sequencing, PCR-single-strand conformation polymorphism, and cloned sequencing methods were used to find genetic variation and identify polymorphisms in these genes. The Mutation Surveyor v3.97 software was used to analyze the sequences. These methods revealed six different sequences of the KAP6 gene, two different sequences of the KAP7 gene, and five different sequences of the KAP8 gene. Accordingly, 3439©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 3438-3445 (2014) Polymorphisms of sheep wool keratin-associated protein genes three (with frequencies >1%) single nucleotide polymorphisms (SNPs) of the KAP6 gene, one SNP of the KAP7 gene, and five SNPs of the KAP8 gene were detected. Interestingly, some of these sequences were present in only certain sheep breeds, thereby suggesting that these special allele sequences could be used as candidate genes of wool characteristics in further studies.

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“…Numerous studies have described variation within both the KAP and KRT genes, including the work of Rogers et al (1994a); Parsons et al (1994a;1996); McLaren et al (1997); Beh et al (2001); Itenge-Mweza et al (2007). There are some reports associating variation in the KRT and KAP genes with variation in wool traits.…”

Section: Previously Published Association Of Genetic Markers With Woomentioning

confidence: 99%

Identification of Polymorphism in the Keratin Genes (KAP3.2, KAP6.1, KAP7, KAP8) and Microsatellite BfMS in Merino Sheep Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) Analysi

Itenge¹

2012

Electrophoresis

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Polymorphism of the KAP1.1, KAP1.3 and K33 genes in Merino sheep

Cited by 45 publications

References 9 publications

Sakız, Kıvırcık ve İvesi Koyun Irklarında KAP 1.1, KAP 1.3 ve K33 Gen Polimorfizmlerinin Belirlenmesi

Sakız, Kıvırcık ve İvesi Koyun Irklarında KAP 1.1, KAP 1.3 ve K33 Gen Polimorfizmlerinin Belirlenmesi

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

Identification of Polymorphism in the Keratin Genes (KAP3.2, KAP6.1, KAP7, KAP8) and Microsatellite BfMS in Merino Sheep Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) Analysi

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