Identification of Polymorphism in the Keratin Genes (KAP3.2, KAP6.1, KAP7, KAP8) and Microsatellite BfMS in Merino Sheep Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) Analysi

Polymorphism of the KAP1.3 and KRT33A genes in the Swakara sheep of Namibia

“…Although RFLPs were the first genetic markers developed, they are losing popularity as a screening method to identify genetic markers because they have the disadvantages of not identifying all of the polymorphism within a length of DNA, are time-consuming, and restriction enzymes and consumables tend to be expensive (Itenge 2012). Since the restriction enzymes only target restriction sites, genetic variations that are not within the restriction sites, are therefore not recognizable by restriction enzymes when using this method.…”

“…Since the restriction enzymes only target restriction sites, genetic variations that are not within the restriction sites, are therefore not recognizable by restriction enzymes when using this method. Itenge (2012), stated that the PCR-SSCP technique offers a rapid, sensitive, relatively inexpensive and simple way to screen for sequence variation, and has become one of the preferred methods for screening samples to detect polymorphism in genes. Numerous studies have reported at least six alleles of the KAP1.3 gene, using PCR-SSCP typing method.…”

“…Genetic markers can either be genes or nonfunctional DNA segments such as microsatellites (Abdul-Muneer, 2014) or minisatellites. According to Itenge (2012), a genetic marker for a particular trait can be defined as a piece of DNA that directly affects a phenotype and shows polymorphism. It can also be a piece of DNA that is closely linked to another piece of DNA that affects a phenotype.…”