Polymorphism of prolactin (PRL) gene exon 5 and its association with egg production in IPB-D1 chickens

Abstract

Abstract. The prolactin (PRL) gene regulates the egg production and incubation in laying chickens. Local chickens' reproductive systems will disrupt as a result of the incubation period activity, and they will lay fewer eggs. This study aimed to determine the prolactin gene polymorphism in IPB-D1 hens and its relationship to egg production. The polymorphism of the exon 5 prolactin gene was examined on 112 samples of the IPB-D1 chicken DNA collection from the Division of Animal Genetics and Breeding, Faculty of Animal Sciences, IPB University. By performing the phenol–chloroform method, the genomic DNA was obtained. A polymerase chain reaction (PCR) product with a size of 557 bp was produced as a result of the DNA amplification. Three single-nucleotide sequences were discovered. Three single-nucleotide polymorphisms (SNPs), g.7835A > G, g.7886A > T, and g.8052T > C, were found in exon 5 of the PRL gene. Each mutation was polymorphic and in Hardy–Weinberg equilibrium. The point mutation g.8052T > C significantly impacted the egg production of IPB-D1 chickens, according to the SNP association analysis on egg production, and may serve as a marker to enhance the selection for the features of egg production in IPB-D1 chickens.

Effect of a Polymorphism in Prolactin Gene on Some Reproductive Traits in TB Crossbred Ducks in Southern Vietnam

“…Investigated from five native duck breeds (Shanma, Shaoxing, Jinyun, Jingjiang and Youxian), Wang et al (2011) reported that polymorphism at PRL/PstI in exon 5 was detected, and ducks bearing CC genotype possessed higher egg production as well as egg weight than those of the CT genotype. Similar results of polymorphism at the PRL/ PstI locus in different duck breeds and its relationship with reproductive traits such as egg production ability was also reported in other studies in ducks (Ghanem et al, 2017;Bai et al, 2019;Rohmah et al, 2022) or in chicken (Cui et al, 2006). In chicken, PRL/PstI +/+ genotype was associated with higher egg production at the age of 40 wks (Nagaraja et al, 2000) or at 48 and 57 weeks of age (Li et al, 2008) than PRL/PstI -/genotype have reported similar trend of results on egg production in this current study.…”

“…pcr aND pcr-rfLp assay A fragment of 536 bp of the PRL gene in exon 5 was successfully amplified in all duck samples and the representative electrophoresis image with the 536 bp fragment was clearly observed in the Figure 2. Similar with current study, many studies have also successfully amplified the target gene on exon 5 of the prolactin gene with various fragment sizes in ducks (Mazurowski et al, 2016;Ghanem et al, 2017;Yurnalis et al, 2019;Purwantini et al, 2020) or in chicken (Rashidi et al, 2012;Mitrofanova et al, 2017;Perdamaian and Daryono, 2020;Rohmah et al, 2022). As shown in Figure 3, polymorphism at the PRL/Pstl with two alleles (C and T) and three genotypes (CC: 536 bp; TT: 406/130 bp and TC: 536/406/130 bp) was clearly distinguished.…”

“…Restriction fragment length polymorphism based on polymerase chain reaction (PCR-RFLP) is a regularly used for polymorphism genotyping studies (Hiyama et al, 2012;Roy et al, 2020). Many candidate polymorphism genes in layer ducks have been recognized and correlated with egg production, in which the prolactin (PRL) gene has important functions in living things, specifically in egg production in duck (Reddy et al, 2002;Susanti et al, 2012;Irma et al, 2014;Mazurowski et al, 2016;Wang et al, 2011;Chuekwon and Boonlum, 2017;Ghanem et al, 2017;Astuti, 2019;Sabry et al, 2020), goose (Tang et al, 2021) and chicken (Cui et al, 2006;Rashidi et al, 2012;Roy et al, 2020;Manoharan et al, 2021;Rohmah et al, 2022). The prolactin gene was identified in ducks at 10 kb in length and comprising 4 introns and 5 exons (Kansaku et al, 2008).…”

“…Research on PRL gene polymorphism in Indonesian local ducks documented exon 2 (Susanti, 2015) and exon 4 (Indriatia et al, 2016) or in chicken (Rohmah et al, 2020). In exon 5, a single nucleotide polymorphism (SNP) showed a positive correlation with egg production in Chinese ducks (Wang et al, 2011), Indonesian local ducks (Susanti et al, 2012;Astuti, 2019;Purwantini et al, 2020;Rafian et al, 2022) and Egyptian duck breeds (Sabry et al, 2020) or in chicken (Rohmah et al, 2022). Current study investigated polymorphism of the prolactin gene in exon 5 to determine the effect of genetic polymorphism on some reproductive traits of BT crossbred ducks.…”

A 24 BP Indel Prolactin Gene Polymorphism and Its Association with Some Reproductive Traits in Color Dual-Purpose VLV Hens in Southern Vietnam

“…In poultry, several candidate genes have been identified showing polymorphism associated with egg production. The prolactin (PRL) gene has important functions in birds, specifically in egg production in chicken (Li et al, 2009;Rowshan et al, 2012;Wilkanowska et al, 2014;Brijendra et al, 2018;Roy et al, 2020;Manoharan et al, 2021;Rohmah et al, 2022), duck (Wang et al, 2011;Chuekwon and Boonlum, 2017;Ghanem et al, 2017;Astuti, 2019;Sabry et al, 2020;Nguyen et al, 2023), goose (Ma et al, 2015;Tang et al, 2021), and quail (Yousefi et al, 2012;Lotfi et al, 2013;Eichie et al, 2016). In chicken, the size of the prolactin gene is 9.536 bp and comprises three parts as (i) three promoters (Kansaku, 2000), (ii) five exons (Ohkubo et al, 1998;Miao et al, 1999;Cui et al, 2004), and (iii) four introns (Dhara and Soller, 1999;Au and Leung, 2002).…”