Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

Bhushan, Bharat; Patra, Biswanath; Das, P. J.; Dutt, Triveni; Kumar, Pushpendra; Sharma, Arjava; Umang,; Dandapat, S.; Ahlawat, Shiv Pal S.

doi:10.1590/s1415-47572007000400009

Cited by 4 publications

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References 10 publications

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“…Among identified immune-related genes, the bovine lymphocyte antigen (BoLA) caught the attention of the researcher, as it was directly involved in antigen presentation. The BoLA gene located on chromosomes BTA 23 [30], and generally known as the MHC of cattle, was reported to play an integral role in immune responsiveness and susceptibility to the diseases of the host animal [31]. MHC is a cell surface glycoprotein molecule, having the binding ability to foreign peptides, such as viral proteins, and provides context for the recognition of T-lymphocytes responsible for cell-mediated immunity [32,33].…”

Section: Discussionmentioning

confidence: 99%

RNA Sequencing (RNA-Seq) Based Transcriptome Analysis in Immune Response of Holstein Cattle to Killed Vaccine against Bovine Viral Diarrhea Virus Type I

Lopez

Santiago

Lee

et al. 2020

Animals

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Immune response of 107 vaccinated Holstein cattle was initially obtained prior to the ELISA test. Five cattle with high and low bovine viral diarrhea virus (BVDV) type I antibody were identified as the final experimental animals. Blood samples from these animals were then utilized to determine significant differentially expressed genes (DEGs) using the RNA-seq transcriptome analysis and enrichment analysis. Our analysis identified 261 DEGs in cattle identified as experimental animals. Functional enrichment analysis in gene ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed the DEGs potentially induced by the inactivated BVDV type I vaccine, and might be responsible for the host immune responses. Our findings suggested that inactivated vaccine induced upregulation of genes involved in different GO annotations, including antigen processing and presentation of peptide antigen (via MHC class I), immune response, and positive regulation of interferon-gamma production. The observed downregulation of other genes involved in immune response might be due to inhibition of toll-like receptors (TLRs) by the upregulation of the Bcl-3 gene. Meanwhile, the result of KEGG pathways revealed that the majority of DEGs were upregulated and enriched to different pathways, including cytokine-cytokine receptor interaction, platelet activation, extracellular matrix (ECM) receptor interaction, hematopoietic cell lineage, and ATP-binding cassette (ABC) transporters. These significant pathways supported our initial findings and are known to play a vital role in shaping adaptive immunity against BVDV type 1. In addition, type 1 diabetes mellitus pathways tended to be significantly enriched. Thus, further studies are needed to investigate the prevalence of type 1 diabetes mellitus in cattle vaccinated with inactivated and live BVDV vaccine.

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Section: Discussionmentioning

confidence: 99%