Association of bovine major histocompatibility complex class I (BoLA-A) alleles with immune response to Brucella abortus strain 19 in calves

Patra, Biswanath; Panigrahi, Manjit; Ahmad, Sheikh Firdous; Dandapat, S.; Kumar, Pushpendra; Bhushan, Bharat

doi:10.1016/j.vetmic.2019.108569

Cited by 3 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These overlapping regions are potentially due to repetitive sequence causing low mapping resolution, primarily in telomeric regions, and were not considered evidence for introgression (Supplementary Table 6 ). Two regions of note are immediately downstream of the bovine major histocompatibility complex (MHC) class II region, DRB3 (BoLA-DRB3), a region that has been associated with susceptibility to several infectious diseases 25 , 26 .…”

Section: Resultsmentioning

confidence: 99%

Genomic evaluation of hybridization in historic and modern North American Bison (Bison bison)

Stroupe

Forgacs

Harris

et al. 2022

Sci Rep

View full text Add to dashboard Cite

During the late nineteenth century North American bison underwent a significant population bottleneck resulting in a reduction in population size of over 99% and a species-level near-extinction event. Factors responsible for this destruction included indiscriminate killing, loss of access to suitable habitat, and diseases. At the nadir of this population crash, very few wild plains bison survived and were restricted to Yellowstone National Park, USA and a small number of wild wood bison remained in Wood Buffalo National Park, Canada. However, most surviving bison in the late 1800’s were maintained by cattle ranchers in private herds where hybridization between bison with various breeds of domestic cattle was often encouraged. Over the last 20 years, the legacy of this introgression has been identified using mitochondrial DNA and limited nuclear microsatellite analyses. However, no genome-wide assessment has been performed, and some herds were believed to be free of introgression based on current genetic testing strategies. Herein, we report detailed analyses using whole genome sequencing from nineteen modern and six historical bison, chosen to represent the major lineages of bison, to identify and quantitate signatures of nuclear introgression in their recent (within 200 years) history. Both low and high coverage genomes provided evidence for recent introgression, including animals from Yellowstone, Wind Cave, and Elk Island National Parks which were previously thought to be free from hybridization with domestic cattle. We employed multiple approaches, including one developed for this work, to identify putative cattle haplotypes in each bison genome. These regions vary greatly in size and frequency by sample and herd, though we detected domestic cattle introgression in all bison genomes tested. Since our sampling strategy spanned across the diversity of modern bison populations, these finding are best explained by multiple historical hybridization events between these two species with significant genetic recombination over the last 200 years. Our results demonstrate that whole genome sequencing approaches are required to accurately quantitate cattle introgression in bison.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic evaluation of hybridization in historic and modern North American Bison (Bison bison)

Stroupe

Forgacs

Harris

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“… 46 It was reported that the variation of BoLA-A host (exons 2–3 and 4–5) was related to the host immune response to B. abortus. 47 After BALB/c mice were infected by B. abortus , the induction of mucosal and systemic immunity showed that the total Ag-specific IgG significantly increased in infected groups. In addition, the spleen cells in the infected group produced IFN-γ and IL-4, indicating induction of both Th1 and Th2 responses.…”

Section: Discussionmentioning

confidence: 97%

Immunological pathways of macrophage response to Brucella ovis infection

Zhou

Luo

et al. 2020

Innate Immun

View full text Add to dashboard Cite

As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis. The DEGs related to immune pathway were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) functional enrichment analysis. Quantitative real-time PCR (qRT-PCR) was performed to validate the transcriptome sequencing data. In total, we identified 337 up-regulated and 264 down-regulated DEGs in B. ovis-infected group versus mock group. Top 20 pathways were enriched by KEGG analysis and 20 GO by functional enrichment analysis in DEGs involved in the molecular function, cellular component, and biological process and so on, which revealed multiple immunological pathways in RAW264.7 macrophage cells in response to B. ovis infection, including inflammatory response, immune system process, immune response, cytokine activity, chemotaxis, chemokine-mediated signaling pathway, chemokine activity, and CCR chemokine receptor binding. qRT-PCR results showed Ccl2 (ENSMUST00000000193), Ccl2 (ENSMUST00000124479), Ccl3 (ENSMUST00000001008), Hmox1 (ENSMUST00000005548), Hmox1 (ENSMUST00000159631), Cxcl2 (ENSMUST00000075433), Cxcl2 (ENSMUST00000200681), Cxcl2 (ENSMUST00000200919), and Cxcl2 (ENSMUST00000202317). Our findings firstly elucidate the pathways involved in B. ovis-induced host immune response, which may lay the foundation for revealing the bacteria–host interaction and demonstrating the pathogenic mechanism of B. ovis.

show abstract

Characterization of BoLA class II DQA and DQB by PCR-RFLP, cloning, and sequencing reveals sequence diversity in crossbred cattle

Parasar

Bhushan

Panigrahi

et al. 2021

Animal Biotechnology

View full text Add to dashboard Cite

Association of bovine major histocompatibility complex class I (BoLA-A) alleles with immune response to Brucella abortus strain 19 in calves

Cited by 3 publications

References 23 publications

Genomic evaluation of hybridization in historic and modern North American Bison (Bison bison)

Genomic evaluation of hybridization in historic and modern North American Bison (Bison bison)

Immunological pathways of macrophage response to Brucella ovis infection

Characterization of BoLA class II DQA and DQB by PCR-RFLP, cloning, and sequencing reveals sequence diversity in crossbred cattle

Contact Info

Product

Resources

About