Polymorphism of Duck HSP70 Gene and mRNA Expression under Heat Stress Conditions

Subekti, Kusnadidi; Solihin, Dedy Duryadi; Afnan, Rudi; Gunawan, Asep; Sumantri, Cece

doi:10.3923/ijps.2019.591.597

Cited by 6 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Various local breeds of livestock, well-known and unknown, can be found in each province with unknown numbers and potentials. These breeds have comparative advantages compared to imported livestock, such as good adaptability to tropical environments and good production characteristics due to of natural selection (Nova et al, 2020;Subekti et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

Rafian

Yurnalis

Fenita

et al. 2022

Jurnal Sain Peternakan Indonesia

View full text Add to dashboard Cite

This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.

show abstract

Section: Introductionmentioning

confidence: 99%

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

Rafian

Yurnalis

Fenita

et al. 2022

Jurnal Sain Peternakan Indonesia

View full text Add to dashboard Cite

show abstract

“…Hal ini menunjukkan bahwa itik Sikumbang Jonti dapat beradaptasi pada lingkungan tinggi dan juga rendah. Hal ini juga didukung oleh penelitian Subekti et al, (2019), yang menyatakan bahwa itik Sikumbang Jonti memiliki daya tahan terhadap cekaman stres panas. Hal ini menunjukkan itik Sikumbang Janti masih dapat dijadikan sebagai ternak itik potensial di Indonesia, dikarenakan Indonesia memiliki iklim tropis atau panas.…”

Section: Ketahanan Stres Panasunclassified

Potensi Itik Sikumbang Jonti Sebagai Itik Lokal Unggul Di Sumatera Barat

Rafian,

Lestari,

Lase

et al. 2023

JP~B

View full text Add to dashboard Cite

Informasi performa itik Sikumbang Jonti, baik sebagai pedaging maupun petelur masih sangat minim. Sehingga perlunya untuk mengetahui informasi tentang itik Sikumbang Jonti, sebagai salah satu itik lokal Sumatera Barat yang dapat dimanfaatkan sebagai itik petelur dan pedaging. Itik Sikumbang Jonti memiliki bobot hidup sebesar 897,8±159,95 gram (selama 8 minggu pemeliharaan) dan 1.486,60 gram (selama 12 minggu pemeliharaan), produksi telur 22,92 ± 13,33 % dengan bobot telur 64,21 ± 2,99 gram/butir selama 9 minggu masa produksi, dapat beradaptasi pada lingkungan tinggi dan juga rendah, memiliki rasio jantan dan betina 1:6, dan memiliki kualitas semen yang baik.

show abstract

“…Ducks are livestock with a high diversity level in terms of types and production potential (Rafian et al, 2023). Ducks also have the potential to be developed because they have reasonably good adaptability (Nova et al, 2020;Subekti et al, 2019) and have efficiency in changing the feed into good meat (Saputro et al, 2016). Moreover, ducks play an important role as producers of eggs (Rafian et al, 2022) and meat to support the availability of cheap and readily available animal protein (Nova et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of Insulin-Like Growth Factor-1 (IGF-1) Gene on Bayang Ducks Using PCR-RFLP Method

Rafian,

Yurnalis

2023

Jurnal Sain Peternakan Indonesia

View full text Add to dashboard Cite

This study aims to determine the polymorphism of the IGF-1 gene in Bayang ducks using the PCR-RFLP method. This study used 120 blood samples of Bayang ducks in Kampung Binuang Dalam, Padang City, West Sumatra Province. Bayang duck blood samples were taken through the brachial vein for ± 1 mL. DNA extraction was taken from the blood using the Genomic DNA Purification Kit (Promega) Protocol. DNA was amplified using a pair of primers F: 5'- CCA GGA ATA TCT TTG GAA GCT GT-3 and R: 5'- TGC TAC GTT ACC AGC CTT GA -3 'which produced a 433 bp fragment of exon 3 IGF-1 gene, and F: 5'- CTG GAG CAG GCA GGA AAA TT - 3 'and R: 5'- TCC AGG GAC AGT GAC TCA AC -3' which produced 801 bp fragments of exon 4 IGF-1 gene. The amplification product was restricted by the DdeI enzyme, which recognized the C↓TNAG slashing site (N = G, A, T, C) for the exon three and MnII regions, which recognized the CAC↓GTG cutting site for the exon four regions. The cutting of the IGF-1 exon three gene product in Bayang ducks using DdeI as a restriction enzyme only recognized the C↓TNAG cutting site. The cutting results visualized with 2% agarose gel showed two genotypes: homozygous (+ / +) of 41 samples and the heterozygote (+/-) genotype of 60 samples. The cutting of the IGF-1 exon four gene product in Shadow ducks using MnII as a restriction enzyme only recognized the CAC↓GTG cutting site. The cutting results visualized with 2% agarose gel showed three types of genotypes, namely four samples homozygous (+/+), 67 samples heterozygous (+/-), and 49 samples homozygous (-/-). Based on the results of the study, it can be concluded that the IGF-1 gene in Bayang ducks is polymorphic, with the frequency of the IGF-1|DdeI genotype being in a hardy-weinberg imbalance, and the IGF-1|MnII genotype in a hardy-weinberg equilibrium.

show abstract

Polymorphism of Duck HSP70 Gene and mRNA Expression under Heat Stress Conditions

Cited by 6 publications

References 8 publications

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods

Potensi Itik Sikumbang Jonti Sebagai Itik Lokal Unggul Di Sumatera Barat

Polymorphism of Insulin-Like Growth Factor-1 (IGF-1) Gene on Bayang Ducks Using PCR-RFLP Method

Contact Info

Product

Resources

About