Polymorphism in Trypomastigotes of Trypanosoma (Megatrypanum) minasense in the blood of experimentally infected squirrel monkey and marmosets

Ziccardi, Mariangela; Lourenço‐de‐Oliveira, Ricardo

doi:10.1590/s0074-02761999000500016

Cited by 14 publications

(9 citation statements)

References 13 publications

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“…has been documented (Hoare, 1972;Ziccardi and Lourenco-de-Oliveira, 1999;Zintl et al 2000;Lainson et al 2008). Trypomastigote pleomorphism was noted in the present study when comparisons were made of morphometric measurements of T. copemani from koalas with those of T. copemani from Gilbert's potoroo and quokka.…”

Section: Discussionsupporting

confidence: 57%

Novel trypanosomeTrypanosoma gillettisp. (Euglenozoa: Trypanosomatidae) and the extension of the host range ofTrypanosoma copemanito include the koala (Phascolarctos cinereus)

et al. 2010

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SUMMARYTrypanosoma irwini was previously described from koalas and we now report the finding of a second novel species, T. gilletti, as well as the extension of the host range of Trypanosoma copemani to include koalas. Phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated that T. gilletti was genetically distinct with a genetic distance (±s.e.) at the 18S rDNA locus of 2·7±0·5% from T. copemani (wombat). At the gGAPDH locus, the genetic distance (±s.e.) of T. gilletti was 8·7±1·1% from T. copemani (wombat). Trypanosoma gilletti was detected using a nested trypanosome 18S rDNA PCR in 3/139 (∼2%) blood samples and in 2/29 (∼7%) spleen tissue samples from koalas whilst T. irwini was detected in 72/139 (∼52%) blood samples and T. copemani in 4/139 (∼3%) blood samples from koalas. In addition, naturally occurring mixed infections were noted in 2/139 (∼1·5%) of the koalas tested.

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Section: Discussionsupporting

confidence: 57%

Novel trypanosomeTrypanosoma gillettisp. (Euglenozoa: Trypanosomatidae) and the extension of the host range ofTrypanosoma copemanito include the koala (Phascolarctos cinereus)

et al. 2010

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“…distance from these two species respectively. T. minasense has been found in neotropical non-human primates from South America (Ziccardi and Lourenco-de-Oliveira, 1999) (Gibson, 2009). Previous studies have reported that a genetic distance of 3.75% at the GAPDH gene is sufficient to delimit a new trypanosome species .…”

Section: Discussionmentioning

confidence: 99%

Trypanosoma teixeirae: A new species belonging to the T. cruzi clade causing trypanosomosis in an Australian little red flying fox (Pteropus scapulatus)

Barbosa

Mackie²,

Stenner

et al. 2016

Veterinary Parasitology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. (Hoare, 1972; Baker, 1973;Marinkelle, 1976Marinkelle, , 1979 Gardner and Molyneux, 1988a, b; Hamanaka and Pinto Ada, 1993; Steindel et al., 1998; Barnabe et al., 2003; Grisard et al., 2003; Lisboa et al., 2008; Cottontail et al., 2009;Maia da Silva et al., 2009; Cavazzana et al., 2010; Garcia et al., 2012; Hamilton et al., 2012; Lima et al., 2012;Lima et al., 2013;Marcili et al., 2013; Silva-Iturriza et al., 2013; Cottontail et al., 2014; Ramirez et al., 2014).In Australia, three Trypanosoma spp. have been described in bats to date:Trypanosoma pteropi from the black flying fox (Pteropus gouldii) (Breinl, 1913; Mackerras, 1959), Trypanosoma hipposideri from the dusky horseshoe bat (Hipposideros bicolor albanensis) and Trypanosoma vegrandis, in pteropid bats (Yangochiroptera) and microbats In the present study, we describe the morphological and genetic characterisation of the novel trypanosome in the little red flying fox (Mackie et al., 2015), for which we proposed the name Trypanosoma teixeirae sp. n. Material and Methods Sample collectionA venous blood sample was collected from the cephalic vein of an adult female little red flying fox that presented to the Australia Zoo Wildlife Hospital (AZWH) in April, 2014.The flying fox had been rescued from the ground at Redcliffe in south-eastern Queensland, Australia and was moribund with anaemia and icterus. Clinical and pathological evidence of disease consistent with trypanosomosis in this flying fox was described by Mackie et al.. Morphological analysesThin blood smears were made from a drop of fresh blood and stained with Diff Quick (Siemens, Germany). After air-drying, the slides were then cover-slipped using DePeX 5 mounting medium Gurr (Merck Pty. Limited, Kilsyth, Victoria, Australia). Stained films were systematically examined using a BX50 microscope (Olympus, Japan) with screen views generated by a DP Controller (version 3.2.1.276, Olympus, Japan). Digital light micrograph images of any trypomastigotes observed were taken at x1000 magnification.Digital images of the organisms identified in the blood films were used to measure key morphological features such as total length (TL), width (W), posterior to kinetoplast (PK), kinetoplast to nucleus (KN), nucleus to anterior (NA) and free flagellum (FF), according to parameters described by Hoare (1972) and Mackerras (1959). Means and standard errors were calculated. The morphological measurements were taken using the software Image J (Abramoff et al., 2004).As two trypanosome species have previously been described in Australian bats based on morphol...

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“…has been documented. Pleomorphism of bloodstream forms of T. minasense has been reported in different experimentally infected hosts, namely squirrel monkeys and marmosets, where the level of variation did not conform to previously measured T. minasense blood forms (Ziccardi and Lourenco-de-Oliveira, 1999). Pleomorphism of bloodstream forms of T. bennetti has also been reported with an increase in in vitro culturing temperature producing a posterior migration of the kinetoplast, affecting the PK values (Kirkpatrick and Terway- Thompson, 1986).…”

Section: Phytomonas Serpens U39577 Herpetomonas Muscarum L18872 85mentioning

confidence: 98%

Trypanosoma irwini n. sp (Sarcomastigophora: Trypanosomatidae) from the koala (Phascolarctos cinereus)

et al. 2009

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The morphology and genetic characterization of a new species of trypanosome infecting koalas (Phascolarctos cinereus) are described. Morphological analysis of bloodstream forms and phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated this trypanosome species to be genetically distinct and most similar to Trypanosoma bennetti, an avian trypanosome with a genetic distance of 0 . 9% at the 18S rDNA and 10 . 7 % at the gGAPDH locus. The trypanosome was detected by 18S rDNA PCR in the blood samples of 26 out of 68 (38 . 2%) koalas studied. The aetiological role of trypanosomes in koala disease is currently poorly defined, although infection with these parasites has been associated with severe clinical signs in a number of koalas. Based on biological and genetic characterization data, this trypanosome species infecting koalas is proposed to be a new species Trypanosome irwini n. sp.

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Polymorphism in Trypomastigotes of Trypanosoma (Megatrypanum) minasense in the blood of experimentally infected squirrel monkey and marmosets

Abstract: Experimental infections by

Cited by 14 publications

References 13 publications

Novel trypanosomeTrypanosoma gillettisp. (Euglenozoa: Trypanosomatidae) and the extension of the host range ofTrypanosoma copemanito include the koala (Phascolarctos cinereus)

Novel trypanosomeTrypanosoma gillettisp. (Euglenozoa: Trypanosomatidae) and the extension of the host range ofTrypanosoma copemanito include the koala (Phascolarctos cinereus)

Trypanosoma teixeirae: A new species belonging to the T. cruzi clade causing trypanosomosis in an Australian little red flying fox (Pteropus scapulatus)

Trypanosoma irwini n. sp (Sarcomastigophora: Trypanosomatidae) from the koala (Phascolarctos cinereus)

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