Polymers for DNA Vaccine Delivery

Zhang, Mingming; Hong, Yanhang; Chen, Wenjuan; Wang, Chun

doi:10.1021/acsbiomaterials.6b00418

Cited by 47 publications

(46 citation statements)

References 223 publications

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“…Optimization of homopolymers and block copolymers for DNA complexes, against non-specific eliminate stability and cells transfection efficient. The complexes are concluding DNA encoding tumor antigen SART3, CD40 and GM-CSF, are immunity intraperitoneally and by inhibiting tumor growth and metastasis to extend the life span of animals carrying CT26 colon cancer [80].…”

Section: Polypeptidesmentioning

confidence: 99%

Applications of polymer-based nanoparticles in vaccine field

Guo

Utupova

et al. 2019

Nanotechnology Reviews

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Polymer-based nanoparticles have good solubility, stability, safety, and sustained release,which increases the absorption of loaded drugs, protects the drugs from degradation, and prolongs their circulation time and targeted delivery. Generally, we believe that prevention and control of infectious diseases through inoculation is the most efficient measure. However, these vaccines including live attenuated vaccines, inactivated vaccines, protein subunit vaccines, recombinant subunit vaccines, synthetic peptide vaccines and DNA vaccines have several defects, such as immune tolerance, poor immunogenicity, low expression level and induction of respiration pathological changes. All kinds of biodegradable natural and synthetic polymers play major roles in the vaccine delivery system to control the release of antigens for an extended period of time. In addition, these polymers also serve as adjuvants to enhance the immunogenicity of vaccine. This review mainly introduces natural and synthetic polymer-based nanoparticles and their formulation and properties. Moreover, polymer-based nanoparticles as adjuvants and delivery carriers in the applications of vaccine are also discussed. This review provides the basis for further operation of nano vaccines by utilizing the polymer-based nanoparticles as vaccine adjuvants and delivery systems. Polymer-based nanoparticles have exhibited great potential in improving the immunogenicity of antigens and the development of nano vaccines in future.

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Section: Polypeptidesmentioning

confidence: 99%

Applications of polymer-based nanoparticles in vaccine field

Guo

Utupova

et al. 2019

Nanotechnology Reviews

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“…Frequently researched diseases include AIDS, 24,[73][74][75][76] Listeria monocytogenes infection, 56,77 respiratory diseases, 54,78-80 B cell lymphoma, 38 herpes simplex virus attacks, 81 viral B hepatitis, 82 renal cell carcinoma, 83 melanoma 84 and others. 85 The PEI-based vaccines work through almost all the known immune routes, and usually mimic the pathogenesis of the diseases themselves. For example, in respiratory infection, intranasal administration was prone to elicit mucosal secretory antibodies, as well as more serum antibodies.…”

Section: Progress Of In Vivo Applications Of Pei-based Vaccinesmentioning

confidence: 99%

“…Moreover, the biodistribution and biodegradation of PEI-based vaccines in vivo, with different sizes and shapes, different administration routes and different modification strategies, still need further investigation. 85,120,121 With breakthrough advances in multidisciplinary fields including chemistry, materials science, immunology and nanotechnology, it is believed that PEIbased vaccines, as well as other new adjuvants that improve the potency of the vaccine with precise modulations, will appear on the list of licensed adjuvants in the near future.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Shen¹,

Li²,

Zhang³

et al. 2017

IJN

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Vaccines have shown great success in treating and preventing tumors and infections, while adjuvants are always demanded to ensure potent immune responses. Polyethylenimine (PEI), as one of the well-studied cationic polymers, has been used as a transfection reagent for decades. However, increasing evidence has shown that PEI-based particles are also capable of acting as adjuvants. In this paper, we briefly review the physicochemical properties and the broad applications of PEI in different fields, and elaborate on the intracellular processes of PEI-based vaccines. In addition, we sum up the proof of their in vivo and clinical applications. We also highlight some mechanisms proposed for the intrinsic immunoactivation function of PEI, followed by the challenges and future perspectives of the applications of PEI in the vaccines, as well as some strategies to elicit the desirable immune responses.

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“…Furthermore, we could observe that some of the FITC labeled proteins and nanotubes were in the same position, which confirms that the proteins were indeed coated on the surface of the nanotubes and could be released after being endocytosed by BMDCs. Moreover, the green fluorescence (OVA) and red fluorescence (endosomes or lysosomes) do not completely coincide in cells treated with Man‐MWCNTs/OVA, indicating that the nanotubes may help antigens escape from endosomes to cytoplasm, which is essential for exogenous antigens to be loaded onto MHC Class I molecules and finally induce CD8 + cell response . All these results demonstrated that compared with OVA and MWCNTs‐COOH/OVA, Man‐MWCNTs/OVA could significantly increase antigen uptake of BMDCs via active surface binding.…”

Section: Resultsmentioning

confidence: 95%

Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells

et al. 2019

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Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor‐specific CD8 + T cells. Carbon nanotubes (CNTs) have attracted great attention in biomedicine because of their unique physicochemical properties. In order to effectively deliver tumor antigens to DCs and trigger a strong anti‐tumor immune response, herein, a specific DCs target delivery system was assembled by using multi‐walled carbon nanotubes modified with mannose which can specifically bind to the mannose receptor on DCs membrane. Ovalbumin (OVA) as a model antigen, could be adsorbed on the surface of mannose modified multi‐walled carbon nanotubes (Man‐MWCNTs) with a large drug loading content. This nanotube‐antigen complex showed low cytotoxicity to DCs and was efficiently engulfed by DCs to induce DCs maturation and cytokine release in vitro, indicating that it could be a potent antigen‐adjuvant nanovector of efficient antigen delivery for therapeutic purpose.

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Polymers for DNA Vaccine Delivery

Cited by 47 publications

References 223 publications

Applications of polymer-based nanoparticles in vaccine field

Applications of polymer-based nanoparticles in vaccine field

Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells

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