Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration

Ayala-Caminero, Radamés; Pinzón-Herrera, Luis; Martinez, Carol A. Rivera; Almodóvar, Jorge

doi:10.1557/mrc.2017.90

Cited by 19 publications

(10 citation statements)

References 174 publications

(188 reference statements)

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“…The electrospinning technique is a versatile and efficient method to generate such matrices using polymeric biomaterials . The efficiency of electrospun scaffolds for improving nerve regeneration has been proved using a variety of synthetic and natural polymers . However, currently, there is still a gap between electrospun biomaterials for nerve repair and the clinical standards of care.…”

Section: Introductionmentioning

confidence: 99%

Accelerated Outgrowth of Neurites on Graphene Oxide-Based Hybrid Electrospun Fibro-Porous Polymeric Substrates

Thampi

Thekkuveettil

Muthuvijayan

et al. 2020

ACS Appl. Bio Mater.

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Fabrication of a surface-engineered electrospun scaffold having biomimetic properties like the extracellular matrix (ECM) is essential for neural tissue engineering. An electroconductive and elastomeric scaffold with aligned fibers acting as a substrate may have a great impact on the directional outgrowth of neurites. In this study, we have electrospun electrically conductive, polyurethane-based elastomeric and topographically aligned fibro-porous neural scaffolds. Adhesive proteins of the ECM are documented to have an important role in controlling neuronal cell behavior, including cell adhesion, proliferation, and neurite outgrowth. These bio-adhesion proteins or nanomaterials mimicking their action, if used for surface modification of neural scaffolds, may have the potential to accelerate the nerve repair process. Thus, electrospun scaffolds fabricated were surface-engineered using a unique and modified single-step electrospraying technique to coat the scaffold surface with an exploratory bio-adhesion agent, a thin layer of graphene oxide (GO) films. The study was then carried out to determine if the GO-coated electrospun electroconductive polycarbonate urethane (PCU) substrate can improve the bio-interface attributes of these scaffolds or may alter the neurite outgrowth of PC-12 cells like any other bio-adhesion proteins. Therefore, the hybrid scaffolds with GO coatings were compared with similar scaffolds coated with poly-l-lysine (PLL) for neural cell adhesion, proliferation, and neurite extension. Neurite outgrowth studies showed that although the average neurite length was comparable on both GO- and PLL-coated surfaces, the length profile of neurites, when categorized based on length, showed an increased number of lengthier neurites on the GO-coated hybrid scaffolds. In particular, the study brings out an innovative surface engineering technique for the coating of GO on polymeric scaffolds. It may be further put together in designing of hybrid surfaces with nanotopographical biophysical cues on three-dimensional neural scaffolds, which in turn may stimulate an accelerated neuronal regeneration via providing an enhanced ECM like milieu.

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Section: Introductionmentioning

confidence: 99%

Accelerated Outgrowth of Neurites on Graphene Oxide-Based Hybrid Electrospun Fibro-Porous Polymeric Substrates

Thampi

Thekkuveettil

Muthuvijayan

et al. 2020

ACS Appl. Bio Mater.

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“…These models are very relevant as they will permit to analyze not only the function of peripheral nerves but mainly to investigate nerve lesion and repair. They will become very useful and promising assays, provided the focus is put on the cell choice, the biomaterials, and the construction of 3D platforms (Ayala‐Caminero et al, ).…”

Section: Pharmacologymentioning

confidence: 99%

Advances in the Ongoing Battle against the Consequences of Peripheral Nerve Injuries

Trejo

2018

The Anatomical Record

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“…So the lesions can have a critical effect on individual performance [1]. When nerves get damaged by severe nerve injury (neurotmesis or axonotmesis) or neurodegenerative diseases (peripheral neuropathy), medical interventions are needed [2]. Herein, the regeneration process is affected by patient age, injury mechanism, proximity to the nerve cell, other diseases, etc.…”

Section: Introductionmentioning

confidence: 99%

Effects of lamellar microstructure of retinoic acid loaded-matrixes on physicochemical properties, migration, and neural differentiation of P19 embryonic carcinoma cells

Ghorbani

Ghalandari

Ghorbani

et al. 2020

Journal of Polymer Engineering

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In this study, retinoic acid loaded-PLGA-gelatin matrixes were prepared with both freeze-casting and freeze-drying techniques. Herein, the effect of unidirectional microstructure with tunable pores on release profile, cellular adhesion, migration, and differentiation was compared. Morphological observation determined that highly interconnected porous structure can be formed, but lamellar pore channels were observed in freeze-casting prepared constructs. The absorption ratio was increased, and the biodegradation rate was decreased as a function of the orientation of microstructure. The in-vitro release study illustrated non-Fickian release mechanism in both methods, so that erosion has predominated over diffusion. Accordingly, PLGA-gelatin scaffolds prepared with freeze-drying technique showed no adequate erosion due to the rigid structure, while freeze-casting one presented more favorable erosion. Microscopic observations of adhered P19 embryonic cells on the scaffolds showed that the freeze-casting matrixes with unidirectional pores provide a more compatible microenvironment for cell attachments and spreading. Besides, it facilitated cell migration and penetration inside the structure and may act as guidance for neuron growth. Improvement in the expression of neural genes in unidirectionally oriented pores proved the decisive role of contact guidance for nerve healing. It seems that the freeze-cast PLGA-gelatin-retinoic acid scaffolds have initial features for nerve tissue regeneration studies.

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Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration

Cited by 19 publications

References 174 publications

Accelerated Outgrowth of Neurites on Graphene Oxide-Based Hybrid Electrospun Fibro-Porous Polymeric Substrates

Accelerated Outgrowth of Neurites on Graphene Oxide-Based Hybrid Electrospun Fibro-Porous Polymeric Substrates

Advances in the Ongoing Battle against the Consequences of Peripheral Nerve Injuries

Effects of lamellar microstructure of retinoic acid loaded-matrixes on physicochemical properties, migration, and neural differentiation of P19 embryonic carcinoma cells

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