Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates

Madaghiele, Marta; Demitri, Christian; Sannino, Alessandro; Ambrosio, Luigi

doi:10.4103/2321-3868.143616

Cited by 250 publications

(151 citation statements)

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“…Considering the whole healing process, the use of soft and wet hydrogels can accelerate wound closure and decrease the possibility of infection and death. In fact, Madaghiele et al (28) reported that hydrogels can absorb and retain wound exudates; in particular, the tight mesh size of hydrogels prevents bacteria from reaching the wound. Conversely, compared to the wound treated with pure poly(NIPAm-MBA) hydrogel, the wound treated with sericin-poly(NIPAm-MBA) hydrogel shows a faster healing rate on day 3.…”

Section: Resultsmentioning

confidence: 99%

Shape Changes and Interaction Mechanism of Escherichia coli Cells Treated with Sericin and Use of a Sericin-Based Hydrogel for Wound Healing

Xue

Liu

Zhang

et al. 2016

Appl Environ Microbiol

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To verify the interaction mechanism between sericin and Escherichia coli, especially the morphological and structural changes in the bacterial cells, the antimicrobial activity of sericin against E. coli as a model for Gram-negative bacteria was investigated. The antibacterial activity of sericin on E. coli and the interaction mechanism were investigated in this study by analyzing the growth, integrity, and morphology of the bacterial cells following treatment with sericin. The changes in morphology and cellular compositions of bacterial cells treated with sericin were observed by an inverted fluorescence microscope, scanning electron microscopy, and transmission electron microscopy. Changes in electrical conductivity, total sugar concentration of the broth for the bacteria, and protein expression of the bacteria were determined to investigate the permeability of the cell membrane. A sericinbased hydrogel was prepared for an in vivo study of wound dressing. The results showed that the antibacterial activity of the hydrogel increased with the increase in the concentration of sericin from 10 g/liter to 40 g/liter. The introduction of sericin induces membrane blebbing of E. coli cells caused by antibiotic action on the cell membrane. The cytoplasm shrinkage phenomenon was accompanied by blurring of the membrane wall boundaries. When E. coli cells were treated with sericin, release of intracellular components quickly increased. The electrical conductivity assay indicated that the charged ions are reduced after exposure to sericin so that the integrity of the cell membrane is weakened and metabolism is blocked. In addition, sodium dodecyl sulfatepolyacrylamide gel electrophoresis demonstrated that sericin hinders the expression of bacterial protein. Sericin may damage the integrity of the bacterial cell membrane, thereby eventually inhibiting the growth and reproduction of E. coli. Compared to sterile gauze, the sericin-based hydrogel promoted fibroblast cell proliferation and accelerated the formation of granulation tissues and neovessels. IMPORTANCEThe specific relationship and interaction mechanism between sericin and E. coli cells were investigated and elucidated. The results show that after 12 h of treatment, sericin molecules induce membrane blebbing of E. coli cells, and the bacteria show decreases in liquidity and permeability of biological membrane, resulting in alterations in the conductivity of the culture medium and the integrity of the outer membrane. The subsequent in vivo results demonstrate that the sericin-poly(N-isopropylacrylamide-N,N=-methylene-bis-acrylamide [NIPAm-MBA]) hydrogel accelerated wound healing compared to that with sterile gauze, which is a beneficial result for future applications in clinical medicine and the textile, food, and coating industries.A lthough many bacteria, such as bifidobacteria and lactobacilli, are beneficial for our daily lives, harmful bacteria like Staphylococcus aureus and Escherichia coli are serious threats to human health due to rapid proliferation and...

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Section: Resultsmentioning

confidence: 99%

Shape Changes and Interaction Mechanism of Escherichia coli Cells Treated with Sericin and Use of a Sericin-Based Hydrogel for Wound Healing

Xue

Liu

Zhang

et al. 2016

Appl Environ Microbiol

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“…Inflammatory reactions, granulation tissue formation, and re-epithelialization are the three classic processes during wound healing [1, 2]. Macrophages, neutrophils, and lymphocytes participate in inflammatory responses in wounds.…”

Section: Introductionmentioning

confidence: 99%

P311 induces the transdifferentiation of epidermal stem cells to myofibroblast-like cells by stimulating transforming growth factor β1 expression

Yao

Wang

et al. 2016

Stem Cell Res Ther

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BackgroundEpithelial to mesenchymal transition, especially to myofibroblasts, plays an important role in wound healing, fibrosis, and carcinogenesis. Epidermal stem cells (EpSCs) are responsible for epidermal renewal and wound re-epithelialization. However, it remains unclear whether and how EpSCs transdifferentiate into myofibroblasts or myofibroblast-like cells (MFLCs). Here, we provide the first evidence showing that P311 induces EpSC to MFLC transdifferentiation (EpMyT) via TGFβ1/Smad signaling.MethodsWound healing and mesenchymal features were observed in the P311 KO and P311 WT mouse model of superficial second-degree burns. After the primary human or mouse EpSCs were forced to highly express P311 using an adenoviral vector, EpMyT was observed by immunofluorescence, real-time PCR, and western blot. The activity of TGFβ1 and Smad2/3 in EpSCs with different P311 levels was observed by western blot. The TβRI/II inhibitor LY2109761 and Smad3 siRNA were applied to block the EpMyT in P311-overexpressing EpSCs and exogenous TGFβ1 was to restore the EpMyT in P311 KO EpSCs. Furthermore, the mechanism of P311 regulating TGFβ1 was investigated by bisulfite sequencing PCR, luciferase activity assay, and real-time PCR.ResultsP311 KO mouse wounds showed delayed re-epithelialization and reduced mesenchymal features. The human or mouse EpSCs with overexpressed P311 exhibited fusiform morphological changes, upregulated expression of myofibroblast markers (α-SMA and vimentin), and downregulated expression of EpSC markers (β1-integrin and E-cadherin). P311-expressing EpSCs showed decreased TGFβ1 mRNA and increased TGFβ1 protein, TβRI/II mRNA, and activated Smad2/3. Moreover, LY2109761 and Smad3 siRNA reversed P311-induced EpMyT. Under the stimulation of exogenous TGFβ1, the phosphorylation of Smad2 and Smad3 in P311 KO EpSCs was significantly lower than that in P311 WT EpSCs and the EpMyT in P311 KO EpSCs was restored. Furthermore, P311 enhanced the methylation of TGFβ1 promoter and increased activities of TGFβ1 5′/3′ untranslated regions (UTRs) to stimulate TGFβ1 expression. P311+α-SMA+ cells and P311+vimentin+ cells were observed in the epidermis of human burn wounds. Also, P311 was upregulated by IL-1β, IL-6, TNFα, and hypoxia.ConclusionsP311 is a novel TGFβ1/Smad signaling-mediated regulator of transdifferentiation in EpSCs during cutaneous wound healing. Furthermore, P311 might stimulate TGFβ1 expression by promoting TGFβ1 promoter methylation and by activating the TGFβ1 5′/3′ UTR.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-016-0421-1) contains supplementary material, which is available to authorized users.

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“…Most researches were documented the hydrogel didn't effect on the process of wound healing, except burn wound healing due to the hydrogel have large amount of water to moist the burn area and prevent the infection (16).…”

Section: +mentioning

confidence: 99%

A Role of Bovine Cardiac Myosin-Hydrogel Polymere to Accelerate Wound Healing of Autograft Skin in Rabbits

2017

IJSR

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The present study were showed more developed in 2 nd and 3 rd treated groups compare with control and 1 st trated groups which were treated by bovine cardiac myosin-hydrogel polymer in three concentration 25%, 50% and 75%, while control group treated with gentamicine and hydrogel only, gross pictures were showed similar in all groups at 1-3 days after surgery, 2 nd and 3 rd groups were loss scar tissue above wound and wound line were showed until 9 days in 3 rd treated group, while wound line in 2 nd group were disappeared until to 10-13 days after surgery. Histopathological changes is very important to evidence the bovine cardiac myosinhydrogel was effect to accelarete wound healing and speed recovery, collagen and fibers matrix in histopathology slides reveal to speed healing and the role of bovine cardiac myosin in wound healing, in conclusion the bovine cardiac myosin is highly effect in wound healing, in recommended ; to more study in this protein specially biochemical study and cytologyical study to knowledge the main elements whose play roles in wound healing.

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Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates

Cited by 250 publications

References 75 publications

Shape Changes and Interaction Mechanism of Escherichia coli Cells Treated with Sericin and Use of a Sericin-Based Hydrogel for Wound Healing

Shape Changes and Interaction Mechanism of Escherichia coli Cells Treated with Sericin and Use of a Sericin-Based Hydrogel for Wound Healing

P311 induces the transdifferentiation of epidermal stem cells to myofibroblast-like cells by stimulating transforming growth factor β1 expression

A Role of Bovine Cardiac Myosin-Hydrogel Polymere to Accelerate Wound Healing of Autograft Skin in Rabbits

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