Polymerase chain reaction with phase change as intrinsic thermal control

Hsieh, Y. F.; Yonezawa, Eri; Kuo, Long-Sheng; Yeh, Shiou–Hwei; Chen, Pei‐Jer; Chen, Ping‐Hei

doi:10.1063/1.4803442

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…Conduction and convection are the primary methods for thermal transfer in most biological nanotechnology-based PCR chips. [1][2][3] The heat capacity of materials must be considered when selecting chip materials because it indirectly affects PCR efficiency. Reducing the sample volume can accelerate heating and cooling, thereby increasing the reaction rate.…”

Section: Introductionmentioning

confidence: 99%

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Hung

Ho²,

Chen

2016

J. Biomed. Opt

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This study developed a microfluidic platform for replicating and detecting DNA in real time by integrating a laser and a microfluidic device composed of polydimethylsiloxane. The design of the microchannels consisted of a laser-heating area and a detection area. An infrared laser was used as the heating source for DNA replication, and the laser power was adjusted to heat the solutions directly. In addition, strong biotin–avidin binding was used to capture and detect the replicated products. The biotin on one end was bound to avidin and anchored to the surface of the microchannels, whereas the biotin on the other end was bound to the quantum dots (Qdots). The results showed that the fluorescent intensity of the Qdots bound to the replicated products in the detection area increased with the number of thermal cycles created by the laser. When the number of thermal cycles was ≥10, the fluorescent intensity of the Qdots was directly detectable on the surface of the microchannels. The proposed method is more sensitive than detection methods entailing gel electrophoresis.

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Section: Introductionmentioning

confidence: 99%

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Hung

Ho²,

Chen

2016

J. Biomed. Opt

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“…In PCR device, a thermoelectric cooler maintains the temperature of the DNA tubes at some specific values (90°C, 55°C and 72°C) for a predetermined amount of time. Denaturation, renaturation and extension are three steps of a periodic cycle performed using PCR temperature control technology [6]. TEC is used to quickly perform these threestage heating-cooling processes [7].…”

Section: Introductionmentioning

confidence: 99%

Design of a Novel PID Controller Based on Machine Learning Algorithm for a Micro-Thermoelectric Cooler of the Polymerase Chain Reaction Device

Arikuşu,

Bayhan

2024

IEEE Access

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“…Polymerase chain reaction (PCR) is a common in vitro DNA replication technique that is widely applied in medical diagnosis, biotechnology, and molecular biology. PCR is divided into three main stages, as follows [4]: (i) DNA denaturation, (ii) primer annealing, and (iii) extension. Two new strands of DNA are formed by being extended from the ends of both primers.…”

Section: Introductionmentioning

confidence: 99%

Laser‐induced heating for in situ DNA replication and detection in microchannels

Hung

Chen²

2018

IET nanobiotechnol.

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This study proposes a method for in situ local deoxyribonucleic acid (DNA) replication and detection in a long DNA strand through laser-induced heating and strong avidin-biotin binding. To achieve the target DNA replication, dielectrophoresis was generated to stretch and immobilise DNA strands on both ends of the electrode. Subsequently, local DNA sequences were replicated using thermal cycles generated by laser-induced heating. Replicated double-stranded DNA products were captured in situ on a solid surface and detected using the fluorescence intensity of quantum dots (Qdots). The results revealed that after six laser-induced thermal cycles, the replicated local DNA sequence could be detected by analysing the difference between Qdot fluorescent intensity before and after replication. The proposed method is expected to improve the efficiency of biosample gene sequence analysis.

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Polymerase chain reaction with phase change as intrinsic thermal control

Cited by 7 publications

References 20 publications

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

Design of a Novel PID Controller Based on Machine Learning Algorithm for a Micro-Thermoelectric Cooler of the Polymerase Chain Reaction Device

Laser‐induced heating for in situ DNA replication and detection in microchannels

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