Laser‐induced heating for in situ DNA replication and detection in microchannels

Abstract

This study proposes a method for in situ local deoxyribonucleic acid (DNA) replication and detection in a long DNA strand through laser-induced heating and strong avidin-biotin binding. To achieve the target DNA replication, dielectrophoresis was generated to stretch and immobilise DNA strands on both ends of the electrode. Subsequently, local DNA sequences were replicated using thermal cycles generated by laser-induced heating. Replicated double-stranded DNA products were captured in situ on a solid surface and detected using the fluorescence intensity of quantum dots (Qdots). The results revealed that after six laser-induced thermal cycles, the replicated local DNA sequence could be detected by analysing the difference between Qdot fluorescent intensity before and after replication. The proposed method is expected to improve the efficiency of biosample gene sequence analysis.

Employing deoxyribonucleic acid (DNA) for personal verification

“…The duplicated local DNA sequence could be recognized after six laser-induced heat cycles. The proposed method should make biosample gene sequence analysis more efficient (Hung & Chen, 2018).…”

DNA Replication of Single Cell Using Laser Heating in Varied Osmotic Solutions

“…Min-Sheng Hung and Ying-Ting Shih Department of Biomechatronic Engineering, National Chiayi University, Chiayi, Chiayi, Taiwan (Republic of China) DNA encodes the genetic information of all the inheritable characteristics of an organic structure. In a previous study, we proposed and demonstrated a microfluidic platform integrated with laser-induced heating for real-time replication and detection of lDNA [1,2]. Avidin-biotin binding was utilized to immobilize one end of the replicated products to the microchannels for the target sequences detection following replication.…”

Application of image recognition for plant virus detection

“…One fluorescent labelling technique that can be applied is the use of quantum dots (QDs), which have narrow emission spectra and stable fluorescent luminance. Additionally, detecting QD fluorescent luminance with a fluorescence microscope is simple and highly sensitive [5,6]. To detect plant virus, we used magnetic beads to be evenly mixed and virus gene sequences captured in the microchannels.…”