Polymerase Chain Reaction Amplification of the Genome of Infectious Bronchitis Virus

Andreasen, James R.; Jackwood, Mark W.; Hilt, Deborah A.

doi:10.2307/1591317

Cited by 21 publications

(21 citation statements)

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“…When the primer used does not recognize all IBVs, false negative results occur (Jackwood et al, 1997;Capua et al, 1998), and are assumed for Lin et al (1991) by Zwaagstra et al (1992). The primers for group-specific RT-PCR tests are, therefore, usually annealed to segments of the IBV in the membrane protein or nucleocapsid protein genes (Andreasen et al, 1991). Results of this same RT-PCR for detection of IBV genomic RNA from allantoic fluid of infected eggs was reported by Jackwood et al (1992).…”

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 92%

“…Subsequently, the c-DNA is amplified many times using the PCR technique. After amplification, the PCR product is to be identified as originating from IBV by another technique such as sequencing (Andreasen et al, 1991;Zwaagstra et al, 1992), restriction enzyme fragment length polymorphism (RFLP) (Lin et al, 1991;Kwon et al, 1993b;Song et al, 1998), or hybridization (Jackwood et al, 1992;Zwaagstra et al, 1992;Kwon et al, 1993a). This confirmation is important to check whether the RT-PCR reaction was specific.…”

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

“…If a group-specific test is required, the RT-PCR must amplify a conserved region of IBV present in all IBV strains (Andreasen et al, 1991). When the primer used does not recognize all IBVs, false negative results occur (Jackwood et al, 1997;Capua et al, 1998), and are assumed for Lin et al (1991) by Zwaagstra et al (1992).…”

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

164

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 92%

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

Section: Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

164

128

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“…EcoM and EcoN primers were synthesized based on previously described primer sequences (MIBV, NIBV) for PCR amplification of IBV RNA [13,14]. EcoM and EcoN primers were identical to MIBV and NIBV primers with the exception that restriction sites in these primers were replaced with EcoRI restriction sites.…”

Section: Rt-pcrmentioning

confidence: 99%

“…A well-characterized region of the coronavirus genome [13][14][15] comprised of nucleotides from both the matrix and nucleocapsid (MN) genes was used as a basis for comparison of three epidemiologically distinct TCV strains and selected coronaviruses.…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of the Matrix/Nucleocapsid Gene Region of Turkey Coronavirus

et al. 1999

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A reverse transcriptase, polymerase chain reaction (RT-PCR) procedure was used to amplify a segment of the genome of turkey coronavirus (TCV) spanning portions of the matrix and nucleocapsid (MN) protein genes (approximately 1.1 kb). The MN gene region of three epidemiologically distinct TCV strains (Minnesota, NC95, Indiana) was amplified, cloned into pUC19, and sequenced. TCV MN gene sequences were compared with published sequences of other avian and mammalian coronaviruses. A high degree of similarity (>90%) was observed between the nucleotide, matrix protein, and nucleocapsid protein sequences of TCV strains and published sequences of infectious bronchitis virus (IBV). The matrix and nucleocapsid protein sequences of TCV had limited homology (<30%) with MN sequences of mammalian coronaviruses. These results demonstrate a close genetic relationship between the avian coronaviruses, IBV and TCV.

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Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan

Wang

Tsai

1996

Archives of Virology

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In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I) in the N-terminus of S1 protein gene were chosen as primers. Sequences of 228-231 base pairs (bp) were amplified by PCR from 25 Taiwan isolates and 4 reference strains (H120, Conn, JMK, Holte). PCR products were digested with 5 restriction endonucleases, BsoFI, DdeI, MboII, AluI, RsaI, and different IBV isolates were grouped according to their RFLP patterns. The RFLP patterns of the 4 reference strains in this study matched the published sequences in GenBank. Except 1 vaccine strain, the other 24 Taiwan isolates were different from these 4 and 18 other IBV strains whose sequences were published. The data from PCR-RFLP and sequencing of IBV genomes showed that the 24 Taiwan isolates can be divided into 2 distinct groups, I and II. Seven RFLP patterns are identified in group I and only 1 in group II.

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Polymerase Chain Reaction Amplification of the Genome of Infectious Bronchitis Virus

Cited by 21 publications

References 0 publications

Detection of infectious bronchitis virus

Detection of infectious bronchitis virus

Sequence Analysis of the Matrix/Nucleocapsid Gene Region of Turkey Coronavirus

Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan

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