Polymer composition primarily determines the protein recognition characteristics of molecularly imprinted hydrogels

Abstract

A hydrogel's molecular recognition properties are determined by the material composition, and are minimally influenced by molecular imprinting.

Rational Design of Immunomodulatory Hydrogels for Chronic Wound Healing

“…Meanwhile, synthetic hydrogels can bind with object proteins using molecularly imprinted polymers (MIPs) method for various therapeutic applications. [ 72 ] Mostly, chemical and physical interactions between the functional groups presented on polymers’ backbone control the crosslinking of the hydrogels, which eventually regulates the degradation rate and mechanical properties of thier networks in physiological environments ( Figure A). For instance, covalent crosslinking is commonly used to develop 3D hydrogel networks suitable for efficient encapsulation of cells and biomolecules such as proteins and growth factors.…”

Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules

“…As discussed below, several attempts have been made at creating MIPs for the recognition of epitopes, peptides, and proteins. Our laboratories have come up with novel methods for the production of materials capable of binding and recognizing specific proteins (44)(45)(46). To minimize the diffusive limitation of a material imprinted in the bulk, we have focused on surface adsorption (47)(48)(49).…”

Molecular imprinting as a simple way for the long-term maintenance of the stemness and proliferation potential of adipose-derived stem cells: an in vitro study

“…38 Molecular imprinting is a synthetic method that creates biomimetic materials for protein recognition. 39 Molecularly imprinted polymers (MIPs) have been proven to promote cell adhesion and proliferation, are stable in various chemical and physiological conditions and can be used multiple times. 40 MIPs are a popular alternative to natural molecular recognition elements, 38,41 and can mimic the antigen-binding properties of antibodies.…”

“…40 MIPs are a popular alternative to natural molecular recognition elements, 38,41 and can mimic the antigen-binding properties of antibodies. 39 Surface imprinting is one of the leading fabrication strategies of MIPs, including different approaches such as soft lithography. In soft lithography, an elastomeric stamp is made by casting a pre-polymer solution, typically polydimethylsiloxane (PDMS) with a relatively low elastic modulus, onto the template.…”