Polymer‐Assisted Hydrothermal Synthesis of Hierarchically Arranged Hydroxyapatite Nanoceramic

Abstract

Flower-like hydroxyapatite (HA) nanostructures were synthesized by a polymer-assisted hydrothermal method. The thickness of the petals/plates decreased from 200 nm to 40 nm as the polymer concentration increased. The thickness also decreased as the hydrothermal treatment time increased from 6 to 12 hr. The HRTEM and SAED patterns suggest that the floral-like HA nanostructures are single crystalline in nature. Structural analysis based on XRD and Raman experiments implied that the produced nanostructure is a pure form of HA without any other impurities. The possible formation mechanism was discussed for the formation of flower-like HA nanostructures during polymer-assisted hydrothermal synthesis. Finally,in vitrocellular analysis revealed that the hierarchically arranged HA nanoceramic had improved cell viability relative to other structures. The cells were actively proliferated over these nanostructures due to lower cytotoxicity. Overall, the size and the crystallinity of the nanostructures played a role in improving the cell proliferation.

Hydroxyapatite for bone related applications derived from sea shell waste by simpleprecipitation method

“…This is the possible mechanism for NHAp nanorod. The mechanism for the formation of HAp nanorod while adding PVP is clearly explained in the literature [18,[57][58][59]. The present study also strongly believed that the above mentioned same mechanism could be involved in the present NHAp.…”

PVP Assisted Synthesis of Hydroxyapatite Nanorods with Tunable Aspect Ratio and Bioactivity

“…The polymers used in the reaction change either the surface chemistry of the parent material or the concentration of soluble species that were already present to grow the crystal [9]. In our previous study, we reported the formation of hierarchically arranged flower like HA nanostructures by a hydrothermal method in the presence of polyacrylamide (PAM) [10]. In this study, we report the successful synthesis of high aspect ratio HA nanorods by hydrothermal method with the addition of polyvinylpyrrolidone (PVP).…”

“…FTIR analysis was performed using a FT-IR spectrometer (PerkinElmer Spectrum 100). Human osteosarcoma cells (MG-63 cells) were cultured to determine the cell viability of PVP assisted high aspect ratio nanorods as described in our previous report [10]. Briefly, cells were cultured at 37 ∘ C in a humidified atmosphere of 5% CO 2 in air containing cell culture medium (DMEM media (phenol red free), Welgene), 10% fetal bovine serum (FBS, Welgene), 2 mM L-glutamine, 1% penicillin, and streptomycin mixture (Antibiotic, Gibco).…”

The pH Effect on the Growth of Hexagonal and Monoclinic Hydroxyapatite Synthesized by the Hydrothermal Method

“…On the other hand, the synthesis conditions must also be considered, such as the precursor's concentration, reaction temperature, reaction time, and pH, since these variables strongly influence the shape and size of the HAp (mainly in the nanometric scale). These last characteristics may dramatically increase the HAp applications, for example, as cancer cells inhibitor as well as in different biomedical and industrial applications, such as a catalyst for Michael type addition, lasers host materials, fluorescence materials, ionic conductors, and gas sensors [13][14][15][16][17].…”