Polyhydroxybutyrate/chitosan/bioglass nanocomposite as a novel electrospun scaffold: fabrication and characterization

Foroughi, Mohammad Reza; Karbasi, Saeed; Khoroushi, Maryam; Khademi, Abbasali

doi:10.1007/s10934-017-0385-2

Cited by 48 publications

(17 citation statements)

References 35 publications

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“…[ 19 20 ] For this reason, biologically active materials such as hydroxyapatite, nano-bioglass (nBG), collagen, or gelatin in combination with chitosan have been developed to improve their mechanical or biological properties. [ 21 22 23 24 25 26 ] Bioactive glasses due to their ability in binding to the surrounding tissues have been widely studied so far. The produced glasses in this way have good conduction and induction of ossification.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

et al. 2018

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Background:The aim of this study was to compare the cytotoxicity and the biocompatibility of three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous dental pulp. Given the importance of scaffold and its features in tissue engineering, this study demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite scaffold using electrospinning method.Materials and Methods:This experimental study was conducted on normal exfoliated deciduous incisors obtained from 6-year-old to 11-year-old healthy children. The dental pulp was extracted from primary incisor teeth which are falling aseptically. After digesting the tissue with 4 mg/ml of type I collagenase, the cells were cultured in medium solution. Identification of stem cells from human exfoliated deciduous teeth was performed by flowcytometry using CD19, CD14, CD146, and CD90 markers. Then, 1 × 104 stem cells were seeded on the scaffold with a diameter of 10 mm × 0.3 mm. Cell viability was evaluated on days 3, 5, and 7 through methyl thiazol tetrazolium techniques (P < 0.05) on different groups that they are groups included (1) PHB scaffold (G1), (2) PHB/chitosan scaffold (G2), (3) the optimal PHB/chitosan/nBG scaffold (G3), (4) mineral trioxide aggregate (MTA), and (5) the G3 + MTA scaffold (G3 + MTA). Data were analyzed with two-way ANOVA at significance level of P < 0.05.Results:The results indicated that the PHB/chitosan/nBG scaffold and PHB/chitosan/nBG scaffold + MTA groups showed significant difference compared with the PHB/chitosan scaffold and PHB scaffold groups on the 7th day (P < 0.05).Conclusion:Thus, it can be concluded that the scaffold with nBG nanoparticles is more biocompatible than the other scaffolds and can be considered as a suitable scaffold for growth and proliferation of stem cells.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

et al. 2018

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“…However, it was determined from the P ‐value results, the coefficients of the model equation and the one parameter at a time graphs that the fiber diameter size decreased linearly with the increase of feed rate as given in Figure D‐F. This may be related to the presence of silver nanoparticles and bioactive glass, which increases the conductivity of the solution . In this case, it is possible that the feed rate cannot overcome the fiber diameter reduction effect of silver nanoparticles and bioactive glass …”

Section: Resultsmentioning

confidence: 99%

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

2020

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The current research focuses on the findings of an investigation on optimizing of an electrospun antibacterial gelatin nanocomposite membrane for bone tissue engineering applications. For this reason, soluble starch coated silver nanoparticles (Ag‐NPs) and bioactive glass particles (BG) were incorporated in to gelatin (Gt) to fabricate Gt/Ag‐NPs/BG nanocomposite membranes. Employing Box‐Behnken design, second‐order models have been successfully obtained to evaluate the statistical significance of individual and interaction effects of applied voltage, tip‐to‐collector distance (TCD), and flow rate on fiber diameter. There was a reasonable agreement between the regression R2 value (0.9542), R2 predicted value (0.8768), and the R2 adjusted value (0.9286) across the entire factor space with identical observations for the experimental and model values. Under optimum conditions (applied voltage of 26 kV, TCD of 180 mm, and flow rate of 0.5 mL/h), the nanocomposite membrane with similar fiber size of bone tissue extracellular matrix can be fabricated with the predicted value of 557 nm obtained by the proposed model. The optimized nanofiber membrane was fabricated under these conditions and average fiber diameter of this membrane was found as 472 ± 94 nm. The characterization studies of this nanofiber suggest that obtained nanocomposite is a potential candidate for bone tissue engineering applications.

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“…The matrix obtained in their work showed different crystallization patterns in comparison with the equivalent matrix of the current work (also containing 25 wt % of Chi) . It suggests that the amount of Chi and the methodology applied to prepare de PHB/Chi materials interfere in the PHB crystallization …”

Section: Resultsmentioning

confidence: 99%

“…The combination of PHB and Chi can be a good alternative to improve the properties of PHB. It is found in the literature that the mechanical properties and potential to be used in tissue regeneration of PHB materials are improved by the incorporation of Chi . In addition, as Chi degradation occurs faster than that of PHB, scaffolds composed of PHB/Chi have a great potential to perform also as drug delivery systems (DDS) …”

Section: Introductionmentioning

confidence: 99%

Swelling degree prediction of polyhydroxybutyrate/chitosan matrices loaded with “Arnica‐do‐Brasil”

Silva

Gobbi

et al. 2019

J of Applied Polymer Sci

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Polyhydroxybutyrate and chitosan have been studied as materials for drug delivery systems (DDSs) due to their biodegradability and biocompatibility. The aim of this work was to produce amphiphilic polyhydroxybutyrate/chitosan matrices that form porous structures (scaffolds) after swelling in water and arnica extract. The matrices were analyzed by TGA, SEM, XDR, DSC, and FTIR. Thin plate spline interpolation method (TPSIM) was used to predict the swelling of the matrices generating three‐dimensional data fitted, showing the influence of time and concentration variables on both fluids absorption. Polyhydroxybutyrate/chitosan samples containing 60 wt % (weight percent) of chitosan formed a porous structure as they were submitted to arnica loading, and the obtained device was able to deliver arnica in physiological medium. Thus, the produced matrices have a strong potential to perform both as scaffolds and DDS © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47838.

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Polyhydroxybutyrate/chitosan/bioglass nanocomposite as a novel electrospun scaffold: fabrication and characterization

Cited by 48 publications

References 35 publications

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

Swelling degree prediction of polyhydroxybutyrate/chitosan matrices loaded with “Arnica‐do‐Brasil”

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