Polyhydroxyalkanoates from organic waste streams using purple non-sulfur bacteria

Montiel-Corona, Virginia; Buitrón, Germán

doi:10.1016/j.biortech.2020.124610

Cited by 28 publications

(19 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the first study reporting the bioconversion of ATB lignin into value-added products. Previous studies have reported the production of short-chain-length PHA from hemicellulose and cellulose fractions of ATB. − However, PHA produced in our work contained approximately 60% of the 3-hydroxydecanoate monomer and 40% of the other medium-chain-length PHA that have been reported to have better thermal and mechanical properties than short-chain-length PHA. , …”

Section: Resultssupporting

confidence: 91%

“…The inoculum and the carbon to nitrogen ratio are widely recognized as some of the most determinant factors for PHA accumulation. , Herein, these initial operating factors were optimized during the two-stage fermentation of ATB lignin to maximize the PHA titer (response variable). A CCD was applied using factorial, axial, and center runs to fit the data to a second order model (eq ) and find the stationary point where the response variable is maximum (optimal value). Y = β ° + ∑ β normali x normali + ∑ β normalii x normali 2 + ∑ ∑ β ij x i x j + ε …”

Section: Methodsmentioning

confidence: 99%

“…37−39 However, PHA produced in our work contained approximately 60% of the 3-hydroxydecanoate monomer and 40% of the other medium-chain-length PHA that have been reported to have better thermal and mechanical properties than short-chainlength PHA. 27,39 Optimization of Fermentation for PHA Accumulation Using ATB Lignin As Substrate. Two-stage fermentation experiments showed that nitrogen content is crucial for PHA accumulation, agreeing with previous reports.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…These characteristics added to their biocompatibility and biodegradability make them ideal for biomedical applications and drug delivery. 26,27 Thus, based on the potential properties of ATB lignin and the characteristics of bioplastics produced by P. putida, the bioconversion of ATB lignin into PHA arises as an attractive process. Recent research highlighted that lignin can be processed with "plug-in" modules or combinatorial pretreatments to fractionate into monomers and oligomers for efficient conversion into PHA.…”

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

Arreola-Vargas

et al. 2022

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Inadequate disposal of Agave tequilana bagasse (ATB) brings environmental and economic issues to tequila producing regions. Recent works have proposed technologies for valorization of the polysaccharide’s fractions of ATB. However, lignin bioconversion has not been investigated due to its recalcitrant nature. Herein, a systematic pretreatment is proposed to release the lignocellulosic fractions and produce medium-chain-length polyhydroxyalkanoates (PHA) from the lignin fraction of ATB. Nuclear magnetic resonance (NMR) analyses revealed that ATB lignin contained 63% of β-O-4 interunit linkages, making this lignin more suitable for pretreatment and biodegradation compared to other lignins. Exploratory experiments revealed that two-stage fermentation is suitable for PHA production from ATB lignin using wild type and engineered Pseudomonas putida strains. PHA increased from 0.09 to 0.39 g/L compared to single batch fermentation. Further improvement to 0.76 g/L was possible by using a central composite design to optimize the inoculum, substrate, and nitrogen concentrations. Finally, PHA depolymerase gene phaZ was knocked out from P. putida and tested at optimal conditions, enabling a PHA titer of 0.97 g/L. Analyses by NMR and GC-MS confirmed lignin derivatives consumption and decrease of β-O-4 linkages. This study lays the foundations to enable agave lignin bioprocessing and opens new avenues for ATB biorefineries.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

Arreola-Vargas

et al. 2022

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…In detail, polyhydroxyalkanoate molecules (PHA) are biodegradable and sustainable coproducts, particularly appealing for biorefinery markets, being useful for many applications currently covered by petroleumbased plastics [64,65]. Several microbial co-cultures of photosynthetic microorganisms and heterotrophic bacteria have been tested and evaluated for PHA bioplastic production [66,67], such as cyanobacteria/heterotrophic bacteria [68], photosynthetic bacteria/microalgae [69], photosynthetic bacteria/heterotrophic bacteria [70] and mixed purple non-sulphur bacteria [71]. Dinesh and colleagues used a combined dark-and photo-fermentation using co-cultures for H 2 and poly-β-hydroxybutyrate (PHB) bioplastic production [72].…”

Section: Manufacturing Of Biomoleculesmentioning

confidence: 99%

Photoautotrophs–Bacteria Co-Cultures: Advances, Challenges and Applications

et al. 2021

View full text Add to dashboard Cite

Photosynthetic microorganisms are among the fundamental living organisms exploited for millennia in many industrial applications, including the food chain, thanks to their adaptable behavior and intrinsic proprieties. The great multipotency of these photoautotroph microorganisms has been described through their attitude to become biofarm for the production of value-added compounds to develop functional foods and personalized drugs. Furthermore, such biological systems demonstrated their potential for green energy production (e.g., biofuel and green nanomaterials). In particular, the exploitation of photoautotrophs represents a concrete biorefinery system toward sustainability, currently a highly sought-after concept at the industrial level and for the environmental protection. However, technical and economic issues have been highlighted in the literature, and in particular, challenges and limitations have been identified. In this context, a new perspective has been recently considered to offer solutions and advances for the biomanufacturing of photosynthetic materials: the co-culture of photoautotrophs and bacteria. The rational of this review is to describe the recently released information regarding this microbial consortium, analyzing the critical issues, the strengths and the next challenges to be faced for the intentions attainment.

show abstract

The application of purple non-sulfur bacteria for microbial mixed culture polyhydroxyalkanoates production

Sali

Mackey

2021

Rev Environ Sci Biotechnol

View full text Add to dashboard Cite

Polyhydroxyalkanoates from organic waste streams using purple non-sulfur bacteria

Cited by 28 publications

References 111 publications

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

Bioconversion of Agave Bagasse Lignin to Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas putida

Photoautotrophs–Bacteria Co-Cultures: Advances, Challenges and Applications

The application of purple non-sulfur bacteria for microbial mixed culture polyhydroxyalkanoates production

Contact Info

Product

Resources

About