The application of purple non-sulfur bacteria for microbial mixed culture polyhydroxyalkanoates production

Sali, Safae; Mackey, Hamish R.

doi:10.1007/s11157-021-09597-7

Cited by 24 publications

(10 citation statements)

References 146 publications

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“…These three genera are often reported as the main microbial groups responsible for PHA production in MMC systems [8,37,[40][41][42][43]. Within the remaining genera present in the biomass microbiome at relative abundances between 1-2.5%, there are also other genera that are reported in the literature as having metabolic constitution enzymes capable of synthesizing PHAs, namely bacteria from the genus Amaricoccus, Bosea, Rhodobacter, or Peptoclostridium [8,37,44,45]. However, these genera seemed to be not so well adapted to the operational conditions imposed and thus were not able to thrive over the well-established PHA-storing microorganisms.…”

Section: Core Bacteriomementioning

confidence: 99%

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

Carvalheira

Amorim

Oliveira

et al. 2022

Life

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Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.

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Section: Core Bacteriomementioning

confidence: 99%

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

Carvalheira

Amorim

Oliveira

et al. 2022

Life

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“…As FSW is the result of the synthetic conversion from syngas to alkanes it contains little to no chemicals other than organics consisting of C, H and O [ 27 ]. Other nutrients such as nitrogen, phosphorus, trace elements, and vitamins are also necessary for PNSB growth [ 43 ], but must be added, making it an ideal substrate for the study of nutrient deficiency.…”

Section: Methodsmentioning

confidence: 99%

Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture

Shaikh

Rashid

Onwusogh

et al. 2023

Biofilm

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“…Table 1 shows AA levels in diverse PNSB species compared to the AA content in mycoprotein from filamentous fungus Fusarium venenatum and the dietary requirement of penaeid shrimp (Penaeus monodon), a typical aquaculture organism for which SCP could be fed. Besides being rich in protein, PNSB-based feeds have a high nutritional quality due to the presence of macromolecules like lipids and carbohydrates and other biomolecules like carotenoids, coenzyme Q10, 5-aminolevulinic acid, and pantothenic acid in PNSB (George et al 2020;Sali and Mackey 2021). In cases where the initial biomass is not utilised solely towards SCP in its intact state, the other biomolecules can be extracted and marketed separately (Lu et al 2013).…”

Section: Comparative Benefits Of Pnsb-based Scpmentioning

confidence: 99%

Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment

Wada

Vincent

Mackey

2022

Rev Environ Sci Biotechnol

Self Cite

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Large-scale production of single-cell protein (SCP) has the potential not only to solve some of the food insecurity and water scarcity crises that plague a significant portion of our world today but also holds the promise to reduce the cost associated with the treatment of industrial and agricultural wastewater. Resource recovery of SCP from organic waste by microbes like yeast and microalgae is commonly documented. However, recently, a class of phototrophic bacteria, purple non-sulphur bacteria (PNSB), has emerged as a favourable option in terms of both wastewater treatment and resource recovery. PNSB are metabolically versatile and tolerant to a wide range of conditions, hence their ability to thrive in diverse waste streams. Besides its rich protein content, PNSB contains other nutritionally valuable bioproducts like carotenoids, coenzyme Q10, 5-aminolevulinic acid, and pantothenic acid. Recent evidence also indicates that PNSB-based aquafeed enhances growth and boosts immunity in certain aquaculture trials. It does not possess the same toxicity as most gram-negative bacteria due to its comparatively less potent lipopolysaccharide composition. With diverse promising prospects of PNSB-based SCP, it is critical to extensively examine the landscape from a holistic standpoint, highlighting the potential challenges large-scale SCP production may pose. Thus, this review explores the comparative advantages of utilizing PNSB for SCP production, essential components of PNSB-based SCP processing, and possible environmental and economic gains associated with the process. Current challenges with PNSB-based SCP production and future outlooks are also examined.

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The application of purple non-sulfur bacteria for microbial mixed culture polyhydroxyalkanoates production

Cited by 24 publications

References 146 publications

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture

Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment

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