Polyethyleneimine–iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA

Hu, Lianxi; Hu, Bo; Shen, Liming; Zhang, Dandan; Chen, Xuwei; Wang, Jianhua

doi:10.1016/j.talanta.2014.10.047

Cited by 36 publications

(16 citation statements)

References 30 publications

(27 reference statements)

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“…At a lower pH value, the existing strong electrostatic attraction could give rise to a favorable adsorption efficiency. This finding coincides with that reported by Fu et al and Hu et al . The pI of BSA is known to be ca.…”

Section: Resultssupporting

confidence: 93%

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Chen

Wang

Song

et al. 2018

Separation Science Plus

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A three‐dimensional reduced graphene oxide porous aerogel with packaged TiO2 nanoparticles was prepared by a simple one‐step self‐assembly in a water bath at a mild temperature. Herein, we overcame the aggregation of graphene layers by forming three‐dimensional architecture, and utilized TiO2 aggregates as blocks to make the composite surface uneven. The extended aromatic areas contribute to DNA adsorption by aromatic stacking and hydrophobic interaction. The obtained composite exhibited porous structure and was positively charged at a low pH. The composite exhibited a high capacity of 890.1 mg g−1 and an adsorption efficiency of 98.4% to DNA at pH 3, however, bovine serum albumin at the same concentration level was hardly adsorbed. The composite showed favorable selectivity to DNA against protein even designed in such a simple manner. The retained DNA could be stripped by using a 0.04 mol L−1 Britton–Robinson buffer at pH 10, giving rise to a recovery of 96.3%. The DNA was isolated from human whole blood to be used as templates for PCR amplification successfully. This illustrates that DNA with high purity and quality was obtained.

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Section: Resultssupporting

confidence: 93%

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Chen

Wang

Song

et al. 2018

Separation Science Plus

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“…DNA isolation was also performed with high efficiency by using amino-modified silica-coated magnetic nanoparticles at high concentrations of phosphate buffer [10]. DNA isolation was performed by using polyethyleneimine (PEI) functionalized FePO 4 nanoparticles with high efficiency in a very short time via electrostatic interactions [11]. Ionic liquid-modified Fe 3 O 4 nanoparticles were also used as a sorbent to investigate the effects of DNA concentration, pH of the sample solution, ionic strength, temperature and contact time in DNA isolation [12].…”

Section: Introductionmentioning

confidence: 99%

Comparative DNA isolation behaviours of silica and polymer based sorbents in batch fashion: monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation

Günal

Kip

Öğüt

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Monodisperse silica microspheres with bimodal pore-size distribution were proposed as a high performance sorbent for DNA isolation in batch fashion under equilibrium conditions. The proposed sorbent including both macroporous and mesoporous compartments was synthesized 5.1 μm in-size, by a "staged shape templated hydrolysis and condensation method". Hydrophilic polymer based sorbents were also obtained in the form of monodisperse-macroporous microspheres ca 5.5 μm in size, with different functionalities, by a developed "multi-stage microsuspension copolymerization" technique. The batch DNA isolation performance of proposed material was comparatively investigated using polymer based sorbents with similar morphologies. Among all sorbents tried, the best DNA isolation performance was achieved with the monodisperse silica microspheres with bimodal pore size distribution. The collocation of interconnected mesoporous and macroporous compartments within the monodisperse silica microspheres provided a high surface area and reduced the intraparticular mass transfer resistance and made easier both the adsorption and desorption of DNA. Among the polymer based sorbents, higher DNA isolation yields were achieved with the monodisperse-macroporous polymer microspheres carrying trimethoxysilyl and quaternary ammonium functionalities. However, batch DNA isolation performances of polymer based sorbents were significantly lower with respect to the silica microspheres.

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“…[11][12][13] Moreover, there is an increasing interest in the employment of PEI for DNA purification. [6,14,15] In this work, the preparation and the characterization of PEI functional surfaces for the adsorption of DNA are presented. The functional surfaces are based on the covalent immobilization of PEI on glass slides.…”

Section: Introductionmentioning

confidence: 99%

XPS analysis of genomic DNA adsorbed on PEI‐modified surfaces

Vanzetti

Pasquardini

Potrich

et al. 2016

Surface & Interface Analysis

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Polyethylenimine (PEI) is a cationic polymer able to interact with nucleic acids. Taking advantage of this property, PEI-modified functional surfaces for the capture of DNA were developed and characterized with X-ray photoelectron spectroscopy (XPS) during every step of the preparation. The XPS demonstrated the presence of PEI on the functional surfaces, while the density of PEI primary amino groups was quantified with a colorimetric test, resulting in 6 10 14 amino groups/cm 2 . Different amounts of DNA were adsorbed on the functional surfaces, and the interaction between DNA and PEI was monitored both via fluorescence and XPS. The functional surfaces were indeed able to capture DNA, opening the possibility of a low-cost and fast strategy for the purification of DNA.

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Polyethyleneimine–iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA

Cited by 36 publications

References 30 publications

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Comparative DNA isolation behaviours of silica and polymer based sorbents in batch fashion: monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation

XPS analysis of genomic DNA adsorbed on PEI‐modified surfaces

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Polyethyleneimine–iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA

Cited by 36 publications

References 30 publications

Reduced graphene oxide aerogel with packaged TiO2 nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Reduced graphene oxide aerogel with packaged TiO2 nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Comparative DNA isolation behaviours of silica and polymer based sorbents in batch fashion: monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation

XPS analysis of genomic DNA adsorbed on PEI‐modified surfaces

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Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood